2011년 강원도 태백에서 최초 발생이 확인 된 사탕무씨스트선충은 고랭지 배추에 경제적 손실을 주는 주요 선충의 하나이다. 또한 2017년 고랭지 배추 재배지역에서 클로버씨스트선충도 분포가 확인 되었다. 본 연구는 씨스트선충의 확산을 조사하기 위하여 2013년부터 고랭지 배추 재배지역에서 씨스트선충의 발생지역 조사를 수행하였다. 아울러 2017년에는 종 특이 프라이머를 이용한 Real-time PCR기법으로 이들 두 선충 이외에 국내 분포가 알려진 콩씨스트선충의 검출지를 강원도 고랭지배추 주산지를 중심으로 조사하였다. 고랭지 배추재배지에서 씨스트선충 류의 감염포장은 매년 증가하여 2017년 태백, 삼척, 정선, 강릉지역에서 분포가 확인되었으며 정선지역이 2017년까지 누적 감염 포장 수가 245 개로 가장 많았다. PCR분석이 가능한 41점의 씨스트선충들 중 61%가 클로버씨스트선충이었으며 사탕무씨스트선충은 9.8%에 불과하였고, 콩 씨스트선충도 29.3% 확인되었다. 따라서 기존에 알려졌던 씨스트선충 검출 포장의 일부는 콩씨스트선충이 감염되어 있을 것으로 보이며 클로버 씨스트선충이 고랭지 배추재배지에 우점하는 것으로 나타나 향후 이 종에 대한 방제 대책이 필요할 것으로 생각된다.

사탕무씨스트선충(Heterodera schachtii)은 최근 우리나라의 배추 재배지의 문제선충이다. 본 연구는 사탕무씨스트선충의 밀도와 배추의 묘 령이 사탕무씨스트선충의 증식에 미치는 영향을 알아보기 위하여 수행하였다. 접종농도가 높은(4마리 유충 또는 알/g 토양) 처리에서 씨스트 수나 암컷 또는 알 수가 유의하게 높았다. 40일 묘령 배추에 고밀도 선충 접종 시 30일이나 60일 후 다른 어린 묘령 배추(파종처리, 20일묘, 30일묘)에 비하여 증식 선충 밀도가 높았다. 높은 선충 밀도 조건에 묘령이 오래 된 모종 식재 시 선충 증식율이 높기 때문에 사탕무씨스트선충 관리를 위해서 는 정식 모종의 묘령 고려가 필요하다.

Sugar beet cyst nematode (SBCN), Heterodera schachtii, is one of the important pests of sugarbeet growing area inthe world, especially in Europe, Canada and USA. SBCN has host in 23 plant families and the important crop host occursin two families; the Chenopodiaceae and Brassicaceae. Host crops include beets, mangolds, cabbage, spinach, brassicas,radish, mustard, and broccoli etc. In korea, SBCN was first detected in the highland Kimchi cabbage fields of easternGangwon-do, 2011. The obvious foliar symptom is poor plant stands and growth, wilting and yellowing. The most easilyrecognized sign of infection is the white to pale yellow and lemon-shaped adult female attached to roots. A survey ofBrassica spp. fields was conducted to determine the distribution of SBCN in Korea. The cyst nematode was only foundedin a restricted highland Kimchi cabbage fields in Gangwon-do. Damage from SBCN may be reduced by employing acombination of various management practies such as prevention of nematode infestation, crop rotation, SBCN resistanttrap crop, weed control and chemical control.

Influences of low concentration of heavy metals (As, Cd, Cu and Pb) on the nematode community structure were examined to assess the changes of the soil ecosystem for a short period of time. Notable increases of heavy metal concentration on the experiment soil were found on the maximum and their 4-1x concentration treatment of all heavy metals after 18weeks. Overall abundance of the nematodes increased regardless of kinds of heavy metal and their concentrations. Also there were no significant differences in the nematode abundance among all treatments of heavy metals except the abundance of c-p 4 in the maximum treatment of Pb. The number of nematode genus found in soils treated with heavy metal solutions also increased compared to that of the genus of the initial soils; however, no significant differences in richness were observed among the treatments of all heavy metals. In maturity and diversity indices of the nematode community, no significant changes occurred in the soils treated with heavy metal solutions with a few exceptions such as MI2-5 and ΣMI2-5 for Pb, and MI2-5 for As. Significant decreases of the ecological indices in the treatments with highly concentrated heavy metal solutions were noticed in MI2-5 and ΣMI2-5 for Pb, and MI2-5 for As, respectively. All of these results is supposed that the maturity and diversity of the nematode community may be decreased in soils contaminated with heavy metals such as Pb and As at relatively low concentrations of a short period of time, which may be derived from differential effects of heavy metals at low concentrations on the growth and development of the nematodes with different c-p values and trophic types. In the analysis of food web structure, only structure index (SI) was significantly lowered in soils irrigated with the heavy metal solutions of Pb and As, but enrichment index (EI) and channel index (CI) were not, suggesting that stressful soil conditions might be provided to the soil food web structure governed by direct toxic effects of heavy metals differential to nematode populations with different trophic type sandc-p values. Analyses of correlation coefficients also suggest that the influence of heavy metals at low concentrations, especially by Pb, maybe most prominent on the nematodes of c-p2-5, including fungivores, providing stressful soil environments.

The toxicity of Kaempferia galanga rhizome-derived methanol extract (RME), powder (RP) and steam distillate (RSD) to Meloidogyne incognita second-stage juveniles (J2) and eggs and their effects on Lycopersicon esculentum germination and growth were examined in vitro and in pot experiments. Results were compared with those of three nematicides. In contact+fumigant bioassays with J2, RME applied at 1, 0.5 and 0.25mg/g soil exhibited 92, 88, and 73% mortality, respectively. The lethality of RME was almost similar to that of carbofuran but lower than that of either fosthiazate or metam-sodium. RSD and RP were less active than RME. In vapor-phase mortality bioassayswith J2, the test materials were effective in closed container than in open one, indicating that mode of delivery was, in part, a result of vapor action. RME, RSD, and fosthiazate treatments resulted in 91, 100, and 95% inhibition of egg hatch at 250μg/ml and 82, 88, and 81% inhibition of egg hatch at 100μg/ml, respectively. In filter-paper bioassays with L. esculentum seed at 8.8μg/cm2, RME and RP did not cause germination inhibition, while RSD and fosthiazate treatments resulted in 84 and 13% germination inhibition. In pot tests, RME and RSD applied at 8mg/g soil reduced galling caused by M. incognita significantly and fosthiazate at 0.02mg/g soil reduced galling completely. Rhizome materials did not cause any adverse effect on growth of L. esculentum, while fosthiazate application caused significantly reduced root weight. K. galanga rhizome-derived materials, particularly methanol extract, merit further study as potential nematicides and hatching inhibitors for the control of M. incognita populations as fumigants with contact action.

During the survey of plant parasitic nematode of Citrus orchard in Korea, Paratrichodorus minor (Colbran, 1956) Siddiqi is founded from the soil of Citrus unshiu Markovich for the first time in Korea. The body length of females is 428.34~506.22 ㎛ long: a = 12.08~22.79, b = 4.02~5.02, V(%) = 38.46~60.68. The excretory pore is located near the base of esophagus. Additionally, the morphological characteristics on P. porosus (Allen, 1957) Siddiqi, 1974 recorded in 1976 on Korea is redescribed in detail.

This study was conducted to investigate occurrence and biodiversity of phytoparasitic nematodes in kiwifruit orchards in Korea. Soil samples were collected from 11 locations of Gosung, Goheung, Namhae, Bosung, Sacheon, Suncheon, Wando, Jangheung, Jeju, Jindo, Haenam from April to August, 2008. Phytoparasitic nematodes were found in 115 among 178 soil samples. The major genera of phytoparasitic nematodes on kiwifruit were Meloidogyne spp.(52.2%), Tylenchinae (42.1%), Tylenchus spp. (9.0%), Helicotylenchus spp. (9.0%) and Tylenchorhynchus spp. (6.7%). Ditylenchus spp., Hirschmaniella spp., Trichodorus spp. and Psilenchus spp. were also detected, even though their frequencies were very low. According to the cultivation period, the phytoparasitic nematodes were found as follows; 16.9% of soils under 5 years of kiwifruit cultivation, 10.7% 6~10 years, 23.0% 11~15 years, 3.9% 16~20 years and 5.6% over 21 years respectively. The frequency of phytoparasitic nematodes is higher in the open field (50.0%) than that in plastic houses (13.5%). The frequency of phytoparasitic nematodes was 36.5% in the field where vegetables were previously cultivated and 12.9% in the field where rice was previously cultivated.

Trophic groups and functional guilds were studied of soil nematodes from the soils of two abandoned mines in Ilkwang and Gunbuk, South Korea to compare nematode communities between the heavy metal contaminated soil and the nonheavy metal contaminated soil. No obvious correlation was found between the level of heavy metal and the total number of nematodes statistically. However the overall densities of bacterial, hyphal, omnivorous and predatory nematodes from the non-heavy metal contaminated soil was higher than those from the heavy metal contaminated soil. Also the densities of c-p 2, c-p 3, c-p 4 and c-p 5 nematodes were higher from the non-heavy metal contaminated soil than those from the heavy metal contaminated soil. MI, MI 2-5 and ΣMI 2-5 were higher, but there were no significant differences.

The nematicidal activity of Kaempferia galanga rhizome-derived materials (methanol extract, steam distillate, and powder) toward M. incognita second-stage juveniles (J2) was examined using residual contact toxicity and pot bioassays. In residual contact toxicity bioassays with J2, the active principles of K. galanga rhizome were identified as the phenylpropanoids ethyl cinnamate (EC, 1) and ethyl-p-methoxycinnamate (EMC, 2) by spectroscopic analysis. EC exhibited 95% and 49% mortality at 0.06 and 0.04 mg/ml toward J2, respectively, whereas EMC showed 98% and 63% mortality at 0.1 and 0.08 mg/ml. In pot tests with J2, K. galanga rhizome methanol extract gave 92% and 82% mortality at 100 and 20 mg/50g soil, respectively. Steam distillate gave 88% and 68% mortality at 85 and 42.5 mg/50g soil, respectively, whereas rhizome powder provided 83% and 62% mortality at 400 and 200 mg/50g soil. K. galanga rhizome-derived materials, particularly EC and EMC, merit further study as potential root-knot nematode control agents or leads because of their great activity as a nematicide.

차나무뿌리썩이선충(Pratylenchus loosi)이 국내에서 처음으로 전라남도 명암군과 제주도 남제주군의 차나무 뿌리와 주변 중에서 발견되었다. 차나무뿌리썩이선충의 두부주름은 2개이며 암컷의 길이는 433-646이고, a ＝ 29.1-37.5, b ＝ 5.1-6.4, c ＝ 15.0-21.3, vulva (%) ＝ 73.0-85.4였다. 수컷의 몸길이는 408-512 였으며 a ＝ 36.1-40.0, b ＝ 4.8-6.7, c ＝ 17.0-19.0, spicule ＝ 14.1-18.0였다. 구침의 길이는 11.6-18.0였으며, 측선의 갯수는 4개였다. 저정낭은 정자로 차 있으며, 모양은 둥근 고치모양 또는 직사각형 모양이다. 꼬리의 형태는 가늘고 끝이 둥글거나 뾰족하다.