The fruit of Kochia scoparia Scharder is traditionally used as a medicinal ingredient to treat allergic skin diseases and inflammatory diseases in China, Japan and Korea. Recently, several studies reported that K. scoparia had potential for the cytotoxicity of human cancer cells. To investigate the anti-cancer effect of K. scoparia on oral cancer and to determine the specific type of cell death induced by MEKS treatment. We investigated the anti-cancer effects of K. scoparia, methanol extract (MEKS) in HSC4 human oral cancer cells. We examined the effects of MEKS on the proliferation rate, cell cycle arrest, 7-AAD-ANNEXIN V double stain, reactive oxygen species (ROS) generation and activation of apoptosis and necroptosis-associated proteins in HSC4 cells. MTT assay results demonstrated that MEKS decreased the proliferation rates of HSC4 cells in a dose-dependent manner with an IC50 value of 45.3 μg/ml. MEKS at 50 μg/ml significantly increased the sub-G1 DNA contents of HSC4 cells to 84.8%, versus untreated cells. However, the activation of apoptosis-associated proteins such as cleaved caspase 3, cleaved caspase 8, cleaved caspase 9 and cleaved Poly (ADP-ribose) polymerase (PARP) did not detect. The level of Bax protein markedly increased in MEKS-treated HSC4 cells. In addition, the cell viability of the DPQ pre-treated HSC4 cells with MEKS treatment was significantly greater than that of MEKS treated-cells. These results suggest that MEKS inhibits cell proliferation and induces necroptosis in oral cancer cells and that MEKS may have potential chemotherapeutic value for the treatment of human oral cancer.

방사선을 투사한 이화명나방의 교미능력 및 경쟁력 조사를 5-7령 번데기에 gamma선을 투사하여 조사하였다. 얻어진 결과는 다음과 같다. 1. 방사선 처리웅충과, 정상적인 수컷에 매일 새로운 미교미자충을 넣어 주었을 때 그들간에 교미 최고회수에는 유의차가 없었다. 2. 교미초일은 정상웅충의 경우 평소 1.5일이며 25.30Krad 처리웅충은 2.4일이었다. 교미 일 간격은 정상웅충이 1.3일인데 대하여 처리웅충은 3.0일이었으며 방사선 투사는 교미력에 영향이 있었다. 3. 정상웅충 1두에 정상자충 5두와 10두씩을 넣어서 정상웅충의 평균교미회수를 조사한 겨로가 1.2회와 2.5회이었다. 4. 교미경쟁력 실험에서 미교미자충 5두에 대하여 방사선 투사 웅충과 정상웅충의 비율을 1:0, 1:1, 2:1, 1:2, 5:1, 1:5, 0:1로 하였을 때 난부화 감소율은 5:1(30Krad)에서 최대의 감소를 보였으며 이때의 난부화율은 이었다. 5. 방사선 투사는 교미능력에 다소의 영향을 미치나 처리웅충에 의한 산란수 감소의 영향을 고려한다면 이화명충에서 웅성불임 기술의 이용은 가능할 것으로 생각된다.

Poongmi is the first ginger variety developed by pure line selection from 54 Korean local accessions. The selected pure line having high quality and high yielding was tested for regional adaptation, and it was superior to those traditional accessions in g