Purpose: The aim of this study was to devleop and apply training program for serve routine of tennis player in an attempt to improve serve sucess rate. Methods: Female collegiate tennis player who have been involved in this sport for more than 12 years of experience as a tennis player in either amateur or backup national players were recruited as subjects in this study. In order to analyze psychological problem of subjects in this study, in-depth interview and questionnaire were applied to them, and any results raised from the interview was used to set up for goal setting based on any factors the subjects required. Routine program for serve success rate improvement developed in expert consultation was applied to the subject once a week for 8 weeks to investigate factors to affect serve accuracy rate and subsequently serve routine program was developed. Selection of subjects, pilot study, routine application, post-analysis and statistical analysis were progressed in the order. Results: As a result of applying serve routine program to improve were success rate, positive imaginery, grid plate and self-talk could be a good stratedgy to transfter negative to positive way of thinking and this may lead to reducing anxiety, improving concentration and slef-confidence for first and second serve success rate. Conclusion: Serve routine program developed in this study may be positively effective to increase serve sucess rate.