We present a multi-dimensional reduction method of the surveyed cube database obtained using a single- dish radio telescope in Taeduk Radio Astronomy Observatory (TRAO). The multibeam receiver system installed at the 14 m telescope in TRAO was not optimized at the initial stage, though it became more stabilized in the following season. We conducted a Galactic Plane survey using the multibeam receiver system. We show that the noise level of the first part of the survey was higher than expected, and a special reduction process seemed to be definitely required. Along with a brief review of classical methods, a multi-dimensional method of reduction is introduced; It is found that the ‘background’ task within IRAF (Image Reduction and Analysis Facility) can be applied to all three directions of the cube database. Various statistics of reduction results is tested using several IRAF tasks. The rms value of raw survey data is 0.241 K, and after primitive baseline subtraction and elimination of bad channel sections, the rms value turned out to be 0.210 K. After the one-dimensional reduction using ‘background’ task, the rms value is estimated to be 0.176 K. The average rms of the final reduced image is 0.137 K. Thus, the image quality is found to be improved about 43% using the new reduction method.

The reactive oxygen species (ROS) generated during the somatic cell transfer nuclear (SCNT) procedures may cause the mitochondrial dysfunction and DNA damage, which may result in restricts the reprogramming of SCNT embryos and play a key direct role in apoptosis. The present study was conducted to investigate the effect of antioxidant treatment during the SCNT procedures on the inhibition of mitochondria and DNA damages in bovine SCNT embryos. The reconstituted oocytes were treated with antioxidants of 25 μM β-mercaptoethanol (β-ME) or 50 μM vitamin C (Vit. C) during the SCNT procedures. In vitro fertilization (IVF) was performed for controls. Mitochondrial morphology and membrane potential (ΔΨ) were evaluated by staining the embryos with MitoTracker Red or JC-1. Apoptosis was analyzed by Caspase-3 activity assay and TUNEL assay, and DNA fragmentation was measured by comet assay at the zygote stage. Mitochondrial morphology of non-treated SCNT embryos was diffused within cytoplasm without forming clumps, while the IVF embryos and antioxidant treated SCNT embryos were formed clumps. The ΔΨ of β-ME (1.3±0.1, red/green) and Vit. C-treated (1.4±0.2, red/green) SCNT embryos were significantly higher (p<0.05) than that of non-treated SCNT embryos (0.9±0.1, red/ green), which similar to that of IVF embryos (1.3±0.1, red/green). Caspase-3 activity was not difference among the groups. TUNEL assay also revealed that little apoptosis was occurred in SCNT embryos as well as IVF embryos regardless of antioxidant treatment. Comet tail lengths of β-ME and Vit. C-treated SCNT embryos (337.8±23.5 μm and 318.7 ±27.0 μm, respectively) were shorter than that of non-treated SCNT embryos (397.4± 21.4 μm) and similar to IVF embryos (323.3±10.6 μm). These results suggest that antioxidant treatment during SCNT procedures can inhibit the mitochondrial and DNA damages of bovine SCNT embryos.

The green peach aphid(Myzus persicae) and sweet-potato whitefly(Bemisia tabaci) biotype Q are serious pests in hot pepper and cucumber. To optimize the formulation type of Clothianidin 1.8% granule, coated and extruded granule formulation types were tested against green peach aphid in hot pepper and sweetpotato whitefly in cucumber. Clothianidin 1.8% granules were incorporated in soil before transplant of hot pepper and cucumber. At recommend dose(540g ai/ha), the efficacy of Clothianidin 1.8% coated and extruded granules were 83.3% and 94.4% and continued until 50 days and 70 days against green peach aphid, respectively. Against sweet-potato whitefly, the efficacy of Clothianidin 1.8% coated and extruded granules were 65.7% and 96.3% at 32 days in cucumber, respectively. Clothianidin 1.8% extruded granule was safe on crops (hot pepper, cucumber, melon, cabbage etc.) at 540g ai/ha (recommend dose) and 1080g ai/ha. The results suggested that Clothianidin 1.8% extruded granule is promising as a best insecticide against green peach aphid and sweet-potato whitefly, because of the high efficacy and low phytotoxicity.

The purpose of this study was the analysis of sperm ability in Specific Pathogen Free (SPE) miniature pig for production of bio-organ. The collected semen was diluted with extender and stored at 17℃ for up to 7 days. The semen samples were evaluated at 0, 1, 3, 5, and 7 days of storage for analysis of sperm ability. Sperm ability was evaluated by examining viability, progressive motility, sperm abnormality and intensity of the sperm membrane. Also, the semen was processed according to the convenient freezing method, and frozen-thawed sperm was evaluated by examining viability, capacitation and acrosome reaction using chlortetracycline (CTC) staining. Motility of spermatozoa of SPF miniature pig was significantly (P<0.05) lower on 3 days or later compared to the Duroc, Yorkshire and Landrace in domestic boar. The percentage of abnormal spermatozoa of Landrace were significantly (P<0.05) higher than in SPF miniature pig, Duroc and Yorkshire that had a similar percentage on 5 or 7 days of sperm storage. The percentage of spermatozoa with coiled tail decreased during the storage period but there were no significant difference. On the other hand, viability of frozen-thawed spermatozoa had a significantly (P<0.05) lower in SPF miniature pig than in other domestic boars. CTC patterns had no significant difference, but SPF miniature pig had higher percentage of capacitated spermatozoa and lower percentage of acrosome-reacted it than domestic boars. Therefore, this study suggest that it is necessary to develop the suitable extender and freezing methods methods for the high viable rate and fertilizing ability in vitro.