Background : With the recent increase in the area of ginseng cultivation in greenhouses, demand for studies of proper cropping patterns in direct seedling of ginseng and varieties of cultivation are increasing, and the needs for clean production of quality ginseng in greenhouses are emerging in connection with the consumer demand for pesticide-free ginseng and the supply and demand of ginseng seedling for cultivation of young ginseng. Therefore, this study is to provide baseline data in improving of quality of ginseng cultivated in greenhouses by examining the contents of ginsenoside in ginseng cultivated in different cropping patterns and different varieties. Methods and Results : As a result of examining the contents of ginsenoside in 3-year old, direct seedling ginseng cultivated in greenhouses in different depths – 10 ㎝, 20 ㎝ and 30 ㎝ - and width – 90 ㎝, 100 ㎝, 110 ㎝ and 120 ㎝ - of furrows, the contents of ginsenoside showed little differences by the depths of furrow in the furrow width of 90 ㎝ but, the highest content of ginsenoside Rb1 was observed in the furrow width of 120 ㎝ and depth of 20 ㎝, while the ginsenoside contents were high in the furrow depth of 20 ㎝ and width of 100 ㎝ and 120 ㎝. The contents of ginsenoside in different varieties of direct seedling cultivation in greenhouses were greater in the following order: Geumgin > Geumsun > Chunpoong, while the contents of ginsenoside Rb1 were observed to be superior in Sunhyang, Gopoong and Geumseon and the contents of ginsenoside Rb2 were greater in the order of Geumgin > Guemsun >　Cheonpoong. Conclusion : Based on the results, the contents of ginsenoside in different cropping patterns of direct seedling cultivation in greenhouses were higher in lower depths of furrow - 10 ㎝ and 20 ㎝ - at furrow widths wider than 100 ㎝. The contents of ginsenoside in different varieties of direct seedling cultivation in greenhouses were higher in varieties of Geumjin, Geumsun and Chunpoong.

Background : The Food industry is trying to replace many synthetic with surfactants with natural alternatives, mainly inspired by consumer’s demand. Saponins are water-soluble small amphiphilie molecules that can be isolated from various natural sources. Especially, concentrated red ginseng extract (CRGE) contains saponins (called as ginsenoside) and they have been shown medical activities to help alleviate pathological symptoms, promote health and prevent potential diseases. In this study, we confirmed the effectiveness of this natural emulsifier for forming and properties of emulsions containing those. Methods and Results : CRGE was supplied D company, located in Geumsan. We examined the interfacial tension, emulsifying activity, fat globule size, dispersion stability, zeta-potential, microscopic observation. The interfacial tension decreased gradually with increasing CRGE concentration. In emulsion systems, as the concentration increases, fat globules sizes also becomes smaller as well as result of dispersion stability evaluated by separation analyzer (i.e., LUMiFuge) showed that it was more stable in emulsions with increasing CRGE concentration. CRGE-coated droplets were stable to droplet coalescence over a range of pH value (2 - 9), salt concentrations (0 - 500 mM NaCl). However, some flocculation was observed under highly acidic (pH 3) and high ionic strength (≧ 400 mM NaCl) conditions, which might be attributed to screening of electrostatic repulsion. Especially, CRGE-coated droplets had high negative charged at basic pH that decreased in magnitude with falling pH. Conclusion : Thus, CRGE have some emulsifying properties as well as medical activities. The results means that emulsions formed with CRGE may be able to replace synthetic surfactants in food industry.

Background : Ginseng is a perennial, and damage occurs due to various diseases and pests. As a result, yield decreases and quality deteriorates. In particular, it is impossible to be repeatedly cultivated due to root rot, soil-borne disease. For this, in order to control root rot and repeatedly cultivate ginseng by using soil fumigation from virgin soil, this experiment was conducted. Methods and Results : This experiment was performed in ginseng farm field, Geumsan-gun, Chungnam province. And total area was 3ha. And the experiment was conducted in soil where apple trees had been grown. Apple trees were rooted up in 2006. And soil was fumigated in May 2007. With regard to soil fumigant, soil was treated with Basamid of 40kg/10a. After soil fumigation, 5 ton/10a of cattle manure fermented for 1 year was put in the soil in June, and then the soil was cared for by being plowed and rotary-tilled as occasion. Ginseng seeds were sowed in the soil cared for in October 2007. And 4-year-old ginseng was harvested in 2011. The soil from which ginseng was harvested was fumigated by the same method in May 2012, and then ginseng seeds were sowed in 1.5ha in October 2012 and in 1.5ha in October 2013. In October 2013, 3-year-old and 4-year-old ginseng was harvested. And ginseng growth characteristics and root rot incidence was examined. It was shown that 4-year-old ginseng yield after the fumigation of virgin soil was nearly 2 times as high as that of conventional virgin soil cultivation. And root rot didn't occur. After that time, the soil was fumigated again. And when ginseng was cultivated, 3-year-old and 4-year-old ginseng yield was 650kg and 960kg per 10a. And it was shown that root rot incidence was 1.3% and 15.3% in 3-year-old and 4-year-old ginseng respectively. Conclusion : This study showed the results where ginseng can be cultivated repeatedly if soil was fumigated again after ginseng was harvested following the soil fumigation from virgin soil so as to control the ginseng root rot and cultivate ginseng repeatedly.

Background : The effective components of Omija(Schisandra chinensis Bailllon) are lignans (schizandrins and gomisins), and this components were contented mostly in seed part on Omija, which have various physiological functionalities such as anti-cancer, anti-inflammatory, and antioxidant activities. Methods and Results : This study was carried out to determine effective condition(CO2, CO2+ethanol) on extraction using supercritical carbon dioxide extraction (SFE) system and to find interrelation on effective components and antioxidant activity of extracts and residues obtained after extraction. Effective components were analysed lignans and phenolic compounds and antioxidant activirty was determined for DPPH radical scavenging ability on methanol extracts of SFE-extract and SFE-residue. On SFE with ethanol, SFE extract was separated two phase, upper(water phase) and lower(oil phase). SFE-extract showed the highest total lignans content(61.36 mg/g, 72.14 mg/g on lower, 50.58 mg/g on upper) and the lowest total phenolic compounds(6.52 mg/100g) and SFE-residue showed the lowest total lignans content(1.45 mg/g) and the highest total phenolic compounds(16.23 mg/100g) by extracted on CO2+ethanol treatment. SFE-residue methanol extract showed the highest DPPH radical scavenging abilities and SFE-extract upper showed the lowest. Conclusion : Thus, this results showed SFE-extract showed the highest total lignans content, but SFE-residue showed the highest DPPH radical scavenging ability although the lowest total lignans content.

Background : Ginseng berry(GB) is useful not only just in growing source but also in functional food source. The ingredients of crops varies with the maturity. So, GB ingredients need to be analyse for optimal harvesting stage of GB against appropriate use. Methods and Results : This study was carried out to determine optimal harvesting stage of GB. GB was harvested 5 day periods from July 12, started harvesting when pollination was 50 days old, until August 1. GB was analysed color, ginsenosides and fatty acids using colorimeter, LC and GC, respectively. As the majority of GB increase, color of freeeze drying GB powder were changed that lightness and yellowness was increased, redness was decreased. Ginsenoside Re, Rb1 and Rb2, major ginsenoside in GB, were increased and Ginsenoside F1, Rk1 and Rg5, minor ginsenoside, were increased for a time and then decreased. Oleic acid, the main fatty acid in GB, was decreased, and linoleic acid and total fatty acid content was increased to July 27 and then decreased. Conclusion : Total ginsenosides content was the highest on August 1 and total fatty acid content was the highest July 27. As the majority of GB increase, ratio of oleic acid on total fatty acid was decreased and linoleic acid was increased. Thus, GB is that the longer a harvest period and the more useful for food source.

Background : Ginseng is a cold-adopting plant that likes cold climate. As the temperature of korea is increasing to the extent that makes it unsuitable for growing ginseng, there is a need arising for developing ginseng breed that can adapt to higher temperature. Out of 76 genetic lines of 4 years old ginseng owned by our research lab, 5 lines with good and high ginsenoside contents were selected. The test was performed to compare their ginsenoside contents and growth characteristics to the existing ones. Methods and Results : A study was done on the growth of 5 lines of ground part with high ginsenoside contents such as GS97-24-2. The results show that the growth of leaf length and width was best for GS98-4-5 and Geumsun. The root weight of the underground part was good, with the growth of 27.8g per ginseng. The root length did not vary across lines. Geumsun and GS97-24-2 showed the same total length. The growth of rootlet was best for GS98-4-5 lines, showing 5.4 rootlets. The total ginsenoside contents of the underground part was highest for American ginseng with 54.31mg/g, followed by GS98-4-5, GS97-24-2, and Geumsun. American ginseng was high in ginsenoside Re and Rb1 in contents compared to Korean ginseng. Although total ginsenoside contents were high, Rg2 and Rf were not detected in American ginseng while Korean ginseng contained good amount of Rg2 and Rf. Conclusion : Base on the results above, the best ground part among the high ginsenoside lines was GS98-4-5. For underground part, the growth of Americal ginseng and Gopoong were the best. American ginseng, which has good underground part growth even in hot season, contained high ginsenoside contents too. On the other hand, Korean ginseng had more variety of ginsenoside compared to American ginseng.

Background : According to medicinal plant standard culture, flower organ should be removed but there is no detail information on flower organ removal in Atractylodes macrocephala Koidzumi. This study was carried out to examine effect of flower organ removal on the increase of roots yield in Atractylodes macrocephala Koidzumi. Methods and Results : ○ Experiment variety : Atractylodes macrocephala Koidzumi. ○ Treatment : ① Flower organ non-cutting ② Flower organ cutting ○ Planting date : April, 2015/ April 25, 2016 ○ Planting distance : 30×20cm/ 30×15cm ○ Experiment place : Ginseng & Medicinal Plant Research Institute(Geumsan-gun, Chungcheongnam-do) Conclusion : The fresh rhizome yield of F. O. C. at a bud treatments were increased 21.0% ∼50.0% compare to F. O. N. C. treatments. The fresh rhizome yield of F. O. C. at a flower treatments were increased 17.4%∼34.6% compare to F. O. N. C. treatments. The fresh weight of above-ground parts of F. O. C. treatments were decreased 77.2%∼65.7% compare to F. O. N. C. treatments. F. O. C.(=Flower organ cutting) / F. O. N. C.(=Flower organ non-cutting).

Background : In recent years, as the ginseng cultivation area using vinyl house is increasing, the demand of farmhouses for the studies of appropriate direct sowing cultivation and ginseng varieties for vinyl house cultivation is increasing, and there is a necessity to produce high quality clean ginseng in vinyl house in tandem with the consumers’ demand for pesticide-free ginseng. Thus, the goal of this research is to determine appropriate ridge height and width when growing ginseng in the vinyl house using direct sowing cropping system. The research was also done to choose ginseng breed for direct sowing cultivation. Methods and Results : The ground part of 4 years old ginseng grown in the vinyl house using direct-sowing cropping pattern was the type that grew well in 100 ㎝ wide ridge rather than 90 ㎝ wide ridge. It also tended to grow well in 20 ㎝ or 30 ㎝ long ridge as opposed to 10 ㎝ long ones. The root weight of the underground part was superior under 90 ㎝ × 20 ㎝(ridge height×ridge width), 90 ㎝ × 30 ㎝, 110 ㎝ × 10 ㎝ and 110 ㎝ × 20 ㎝ cultivation area. The root diameter was longer, the longer the ridge height. The volume per 1.62 ㎡ was superior for 100cm, 120 ㎝ × 10 ㎝ cultivation area. The ginseng whose ground part was superior in the vinyl house were Chungsun and Seonweon. Seonweon and Gumpoong had superior root weight in underground parts. The root length was superior for Geumsun. The ginseng that were direct sowing in the vinyl house did not have rusty ginseng problem regardless of ridge height and kind. The root injury was greatest in the cultivation area with ridge width of 90 ㎝. Conclusion : According to the results above, the growth in vinyl house using direct sowing cropping pattern was best when ridge width was 100㎝ and 110cm in ridge height of 30 ㎝. The best selection was Chunpoong and Seonweon. The root weight of the underground part was superior in 110 ㎝ × 10 ㎝ cultivation area, the best variety was Seonweon and Gumpoong.

Background : Platycodon grandiflorum root(PGR) was one of the primary herbs used in a phlegm-relieving herb from the past. Purified platycoside compounds from the roots of PGR may exhibit neuroprotective, antimicrobial, anti-inflammatory, anti-cancer, anti-allergy, improved insulin resistance, and cholesterol-lowering properties. To evaluate preference and functionality of PGR extracts, PGR was fermented by several lactic acid bacteria. Lactic acid bacteria used were Leuc. mesenteroides N12-4 and N58-5, L. plantarum N76-10 and 56-12, L. brevis N70-9 and E3-8. Methods and Results : This study was performed in order to investigate the changes of platycosides, as well as the antimicrobial activities on bronchus diseases inducing bacteria(C. diphtheriae, K. pnneumoniae, S. aureus, S. pyogenes) of Platycodon grandiflorum root(PGR) fermented by using lactic acid bacteria(Leuc. mesenteroides N12-4, Leuc. mesenteroides N58-5, L. plantarum N76-10, L. plantarum N56-12, L. brevis N70-9, L. brevis E3-8). Growth of L. plantarum on PGR was the most active during lactic acid fermentation by some different strains. Total platycoside, platycoside E, platycodin A, polygalacin D2, polygalacin D and diapioplatycoside E contents of PGR fermented for 96 hours at 37℃ by Leuc. mesenteroides and L. plantarum were increased, while platycodin D and platycodin D3 were decreased. The antimicribial activity on PGR fermented by L. plantarum N56-12 exhibited a strong microbial proliferation in all four kinds of bronchus diseases inducing bacteria and was higher than non-fermented PGR extract. Conclusion : Thus, this results showed antimicrobial activities on bronchus diseases inducing bacteria and platycosides content of PGR by L. plantarum N56-12 were higher than non-fermented PGR extract.

Background : New ginseng variety “Geumwon” is appropriate for the cultivation in Chungnam. As an effort to develop new ginseng variety for regional specialization, it was collected from the Geumsan ginseng fields, selected and fixed and then was registered as new ginseng variety in 2015 through the verification of production capacity, farm demonstration and regional adaptation test. It is excellent in underground growth, disease-resistance and yield-ability compared to candidate varieties. Methods and Results : Regional adaptation tests were conducted for new ginseng variety “Geumwon” in 3 regions(i.e. Geumsan, Gochang and Icheon). The results suggested that the leaf length and width of 2-year old ginseng was 7.9cm and 4.2cm in 3 regions on an average basis. Anthrax incidence rate was about 2.5%, which was smaller than that of candidate variety(3.6%), and the underground root weight was 5.6g, which was 34% higher than that of candidate varieties. The leaf length and width of 3-year old ginseng was 9.4cm and 4.5cm, and anthrax incidence rate and leaf soot incidence rate was 0.8% and 1.2% respectively, which was lower than that of candidate varieties. The the root weight, root diameter and hull length of “Geomwon” were superior to that of candidate varieties by about 24%, 6% and 9% respectively. In addition, red discoloration and root rot incidence rate were also lower than that of candidate varieties. The growth of 4-year old ginseng was also superior to that of candidate varieties in the order of Geumsan, Ichoen and Gochang, and the anthrax and leaf soot incidence rate was also lower than that of candidate varieties(about 2.2.% and 2.3 %, respectively). Conclusion : The results of this study suggested new ginseng variety “Geumwon” is easy to cultivate in Chungnam because of its superior underground growth and excellent quality, and it was also evaluated to be an excellent variety that would contribute to the specialization of the ginseng cultivated in Geumsan.

Background : Jiwhang (Rehmannia glutinosa L.) cultivation area of chungnam province was 62ha(219 farms, 53.9% of domestic cultivation area, M.A.F.R.A. 20142)). Recently, jiwhang is rapidly spread to 150ha ( Geumsan administration statistics, 2015) as taking place of income crop in Chungnam. High quality jiwhang GAP production and improvement of the distribution system including magnus customers, producer association fosterage and conventional market Vitalization were the reason of rapid cultivation area increase of Rehmannia glutinosa L. in Geumsan. This study was performed to set up the standard Rhizome size for planting in Rehmannia glutinosa L. Materials and Methods ○ Experiment variety : Togang ○ Treatment(rhizome length) : ① 1cm ② 2cm ③ 3cm ④ 4cm ⑤ 6cm(control) ○ Planting date : May 6, 2015 ○ Planting distance : 30×15cm ○ Experiment place : Ginseng and Medicinal Plant Research Institute (Geumsan-gun, Chungcheongnam-do) Conclusion : In the aboveground growth was no significant difference among treatments. In the root characteristics, there was no significant difference among treatments. Take a 1∼2cm rhizome planting saved rhizome amount and planting time to 73.8%∼64.3% and 68.4%∼ 57.8% respectively than 6cm(control).

Background : Generally, price of Jiwhang(Rehmannia glutinosa L.) is highest in August. In this study, Economic Evaluation and Productivity of 9 Jiwhang varieties w ere analyzed according to harvesting tim es of August and October. Materials and Methods ○ Experiment variety : Jiwhang 1, Korea jiwhang, Daegyung, Gogang, Togang, Dagang, Wongang, Hwanggang and Yeongang ○ Treatment : ①Initial-August harvest ②Mid-August harvest ③Ended-August harvest ④Ended-October harvest ○ Planting date : April 17, 2015 ○ Planting distance : 30×15cm ○ Experiment place : Ginseng and Medicinal Plant Research Institute (Geumsan-gun, Chungcheongnam-do) Conclusion : The higher roots yield at August harvest was in Gogang, Dagang and Togang. The lower roots yield at August harvest was in Korea jiwhang and Hwanggang. The higher economic feasibility at August harvest was in Gogang, Togang, Dagang and Wongang. The higher economic feasibility at ended-October harvest was in Gogang, Yeongang, Togang, Dagang and Wongang.

Background : According to medicinal plant standard culture, flower stalk should be removed at the stage of 4-5 main leaves appearance but there is no detail information on stalk removal in jiwhang (Rehmannia glutinosa L.). This study was carried out to examine effect of flower stalk removal on the increase of total yield in Rehmannia glutinosa L. Materials and Methods ○ Experiment variety : Korea jiwhang, Wongang ○ Treatment : ① Peduncle non-cutting ② Peduncle cutting ○ Planting date : April 18, 2015 ○ Planting distance : 30×15cm ○ Experiment place : Ginseng and Medicinal Plant Research Institute (Geumsan-gun, Chungcheongnam-do) Conclusion : Aerial part growth was no significant difference according to treatments in Wongang and aerial weight was havier in Peduncle cutting than in Peduncle non-cutting. Aerial part growth was no significant difference according to treatments in Korea jiwhang. The roots yield in Korea jiwhang was increased about 5.2% in Peduncle cutting. The roots yield in Wongang was increased about 24.9% in Peduncle cutting.

Background : Bai Zhu is generally harvested in 1-2 years after seedstock planting. Recently mainly in Guemsan, Bai Zhu is harvested in just 1 year after planting in spring . According to medicinal plant standard culture, Bai Zhu should be directly sown by 20cm distance in April. This study was carried out to find out proper planting date. Materials and Methods ○ Experiment variety : Bai Zhu (Atractylodes macrocephala Koidz.) ○ Treatment : Planting Date ① Late march ② Early april ③ Middle of april(control) ④ Late april Plantind density : ① 30×10cm ② ① 30×20cm ○ Planting type : Direct Seeding ○ Experiment place : Ginseng and Medicinal Plant Research Institute (Geumsan-gun, Chungcheongnam-do) Conclusion : Number of lateral branches in 30×10cm was more in late march than late April and aerial part growth was superior as planting date was earlier. Emergence rate was higher in middle of April and late April and aerial part weight, fresh root weight, dry root weight rate, and dry root weight were superior as planting date was earlier. Dry weight yield increased as 38.3% in late March compared to middle of April. Dry weight was havier as 36.2% in planting time of late March and early April than planting time of middle of April and late April. Total income was higher in planting time of late March and early April than planting time of middle of April and late April.

Background : Jiwhang (Rehmannia glutinosa L.) cultivation area of Chungnam province was 62ha (219 farms, 53.9% of domestic cultivation area, MAFRA. 20142)**). Recently, jiwhang is rapidly spread to 150ha (Geumsan administration statistics, 2015) as taking place of income crop in Chungnam. High quality jiwhang GAP production and improvement of the distribution system including magnus customers, producer association fosterage and conventional market vitalization were the reason of rapid cultivation area increase of Jiwhang in Geumsan. But low emergence rate caused by spring drought should be solved. This study was carried out to obtain basic data for emergence rate improvement in jiwhang. Materials and Methods ○ Experiment variety : Korea jiwhang ○ Treatment : ①Control ② 1 time irrigation at planting time ③1 time irrigation at planting time + transparent vinyl mulching ○ Planting date : April 30, 2015 ○ Planting distance : 30×15cm ○ Experiment place : Ginseng and Medicinal Plant Research Institute (Geumsan-gun, Chungcheongnam-do) Conclusion : There was no significant difference in the aboveground growth among treatments. The seedling emergence rate was higher in 1 time irrigation at planting time + transparent vinyl mulching(1ITVM) than control or 1 time irrigation at planting time(1I). Aerial part weight was significantly havier in 11ITVM than control or 1I. The roots yield was also more in 1ITVM than control or 1I. According to economic analysis, total income in 1ITVM compared to 1I and control was 125.1% and 54.0% higher respectively.

Background : The authors of this study analyzed the effect of the green manures cultivated in the soil of the preparation field in order to select the green manures suitable for ginseng cultivation and the effect of the green manures on the variation of ginseng growth development, the content of crude saponin and yield ability with the aim to use the results as the primary data for improving the quality of ginseng. Methods and Results: To analyze the effect of the application of green manures on the growth of ginseng, the authors cultivated 4 varieties of green manures and installed blue polyethylene sheet and one-layered black polyethylene net+three-layered blue polyethylene net(OBPNTB) and sun-shading material on the managed soil by re-applying green manures and then cultivated ginseng. The results suggested that the growth of ginseng was excellent in the barley+hairy, barely and rye cultivation plot in the plot treated with OBPNTB rather than the cultivation plot teated with Blue polyethylene sheet(BPS) and two-layered blue & two-layered black polyethylene net(TBTBPN), and the growth of leaf length and stalk diameter was excellent in barely + hairy vetch material. For the underground root weight, the growth was the most excellent(30.19g) in the barley+ hairy vetch cultivation plot treated with OBPNTB. For the underground red discoloration and root rot, the incidence rate was the lowest in the plot treated with BPS in which the water leak in a levee was small than in the test plot treated with sun-shading net. For the ginsenoside content in the cultivation plot treated with TBTBPN, the ginsenoid content was the highest in the rey cultivation plot, and it was the highest in the cultivation plot treated with OBPNTB. Conclusion : The results of study suggested rye among green manures is the most suitable for the growth of ginseng; underground growth was the most prominent in the plot treated with sun-shading net material (black 1 + blue 3) and the underground ginsenoside content was the most prominent in the rye cultivation plot and the plot treated with the sun-shading sheet.

Background : In recent years, as the ginseng cultivation area using vinyl house is increasing, the demand of farmhouses for the studies of appropriate direct seeding cultivation and ginseng varieties for vinyl house cultivation is increasing, and there is a necessity to produce high quality clean ginseng in vinyl house in tandem with the consumers’ demand for pesticide-free ginseng and the supply-demand of ginseng seedling for sprout ginseng. Thus, the authors of this study analyzed the change of photosynthetic properties according to the cropping pattern and varieties with the aim to use the results of this study as the primary date for improving the quality of ginseng cultivated in vinyl house. Methods and Results : The authors analyzed the change of photosynthetic properties for the 3-year old ginseng cultivated through direct seeding according to the ginseng field levee height (10, 20, 30cm) and width (90, 100, 110, 120cm). The results suggested that with an increase in light intensity, the photosynthesis tends to increase regardless of levee height and width. When the levee width was 90cm, the photosynthesis increased as the levee height was reduced and it increased as the levee height increased when the levee width was 100cm. The photosynthesis was high when the levee height was 20cm for the levee width of 110cm and 120cm. The photosynthesis was analyzed for 11 varieties of 3-year old ginseng cultivated through direct seeding. The results suggested that the photosynthesis tends to increase as the light intensity increased in all 11 varieties, and the photosynthesis was the highest at 500PAR(ray source: 3.23umolCO2m-2s-1㎛ol) in the order of Cheonpoong, Yeonpoong, Cheongson and Sonwoon. Conclusion : The results of this study suggested that the photosynthesis according to the direct seeding cultivation in vinyl house increased as the levee height decreased for the levee width of 90cm and it was high when the levee height was 20cm for the levee width of 120cm. For the ginseng variety, Sonwon and Cheonpoong showed the highest photosynthesis.

Background : This study was carried out to investigate the changes to fatty acid, mineral, and ginsenosides contents in ginseng seed when they were stratified for different length of time and to determine whether variety had any effects on the changes. The aim was to improve the ginseng seed stratification process. Methods and Results : The ginseng varieties used were Geumpoong, Chunpoong, Yunpoong, and K-1. Stratifying periods treated on ginseng seed were 0, 20, 40, 60, 80, and 100 days. The main fatty acids of ginseng seed were oleic acid (C18 : 1, n9c) with a content of 78.40 - 79.20% followed by linoleic acid (C18 : 2, n6c). The main mineral in the seeds was potassium (K), at 1208.2 - 1337.6 ㎎/100 g. The main ginsenosides in ginseng seed were ginsenoside Re and Rb1. Increasing the length of the stratification periods led to increases in oleic acid content (60 - 80 days), however after this the content declined. In contrast, linoleic acid content fell as the stratification period increased. K, P, Mg, Ca and Na content rose as the stratification period increased. The ginsenoside Re content of Chunpoong and K-1 cultivar seeds also rose as the stratification period increased which meant that total ginsenoside content increased. However, ginsenoside Re content rose in Geumpoong and Yunpoong seeds, but total ginsenoside content decreased as the stratification period increased. Conclusions : Some beneficial compound in ginseng seed rose as the stratification period increased. Therefore, ginseng seed stratification could improve the food value of ginseng.