Under normal conditions, women produce a single dominant follicle that participates in a single ovuation each menstrual cycle. But Polycystic ovary syndrome(PCOS) conditions, folliculogenesis does not proceed normally. This condition leads to the accumlation of large numbers of small graffian follicles in which the theca interstitial cells (TIC) produce abnormally large amounts of androgen. PCOS is probably the most common endocrine disorder, affecting women of reprodutive age with 5-10% prevalence estimate. Chronic anovulation, hyperandrogenism, hirsutism, obesity, infertility and polycystic ovaries are clinical hallmarks of women with PCOS. Its etiology remains unknown. To investigate the gene expression pattern of ovary in PCO-induced rat, we used cDNA expression analysis. Total RNA was extracted from the ovary of PCO-induced rat and reverse-transcribed in the presence of［P］-dATP Which were hybridized to Atlas Rat Toxicology 1.2 array (Clontech) representing approximately 1176 rat genes. We compared gene expression between ovary of pco-induced immature female rats and control. Differential gene expression profiles were revealed (LIFR-alpha, ADRA1A, Heat shock 90-kDa protein A, PDGFRA). Reverse transcription-polymerase chain reaction(RT-PCR) was used to validate the relative expression pattern obtained by the cDNA array. The precise relationship between the altered expression of genes and PCO is a matter of further investigation. This study was supported by Korea Science and Engineering Foundation(KOSEF)

Stroke occurs when local thrombosis, embolic particle or the rupture of blood vessele interrupts the blood floe to the brain. -estradiol 17-valerate has been reported to exert neuroprotective effects when administered before an ischemic insult. Recently, the pathophysiology of cerebral ischemia has been studied extensively in rat with various methods. In the present study, we investigates whether -estrodiol 17-valerate can protect against brain injury. RNA sample were extracted from the hippocampus of female rat, reverse-transcription in the presence of [32p] dATP. Differential gene express-ion profiles were revealed (Bone morphogenetic protein type 1A receptor, Protein disulphide isomerase, Leukemia inhibitor factor receptor, cytochrome bc- 1 complex-x core P, thiol-specific antioxidant protein). RT-PCR was used to validate the relative expression pattern obtained by the cDNA array. The precise relationship between the early expression of recovery genes and stroke is a matter of luther investigation. This Study was supported by the Korea Science and Engineering Foundation(KOSEF) through the Biohealth Products Research Center(BPRC), Inje University, Korea.

본 연구의 목적은 ADHD 아동의 운동 프로그램 참여에 따른 그들의 기분상태와 인지기능의 변화를 단일 피험자 연구 설계법을 통하여 장기간 심층적으로 분석하는데 목적이 있다. 연구참여자는 연구의 목적에 부합토록 실험 시작 당시 서울 소재 C대학교 종합병원 소아정신과에서 ADHD로 판명되어 치료중인 초등학생 4명을 대상으로 하였다. 측정도구로는 Rejeski(1985)가 개발한 기분상태 척도(Feeling Scale: FS)와 인지기능을 측정하기 위해 Wechsler(1991)가 제작한 웩슬러 아동지능검사(WISC-Ⅲ:Wechsler Intelligence Scale for Children-Third)를 곽금주, 박혜원과 김청택(2001)이 표준화한 한국 웩슬러 아동용 개인 지능검사 도구(K-WISC-Ⅲ)를 사용하였다. 본 연구의 실험설계는 단일피험자 설계(single subject design) 유형 중철회 설계(withdrawal design)의 대표적인 A1-B1-A2-B2 설계법을 채택하여 총24주 동안 심층적으로 분석되었다. 본 연구의 연구절차를 통하여 도출된 결과는 다음과 같다. 첫째, ADHD 아동의 운동 참여는 그들의 기분상태를 긍정적으로 증가시키는 것으로 나타났다. 둘째, ADHD 아동의 운동 참여는 그들의 인지 기능 중 집중력과 처리속도에 긍정적 효과가 있는 것으로 나타났다. 이러한 운동 처치 프로그램의 효과들은 단일피험자 설계법에 의거하여 A2단계(운동철회)와 B2단계(2차 운동처치)에서 재차 검증되었으며 타당성을 확보하였다. 본 연구의 결과들은 ADHD 아동의 운동 참여에 따른 다양한 효과를 확장시키고 운동의 효과를 일반화 시키는데 기초자료로서 활용될 수 있을 것이다.