The effects of additions/deletions in glycosylated residues of recombinant human EPO (rhEPO) produced in CHO-K1 on their secretion were examined. hEPO cDNA was amplified from human liver mRNA and cloned into the pCR2.1 TOPO. Using overlapping-extension site-directed mutagenesis method, glycosylation sites at 24th, 38th, 83rd, and 126th were respectively or accumulatively removed by substituting its asparagine (or serine) with glutamine. To add novel glycosylation sites, 69 and 105th leucine was mutated to asparagine. Mutant and wild type rhEPO constructs were cloned into the pcDNA3 expression vector with CMV promoter and transfected into CHO cell line, CHO-K1, to produce mutant rhEPO mutant rhEPO proteins. Enzyme-linked immunosorbant assay (ELISA) and Western analysis with monoclonal anti-EPO antibody were performed using supernatants of the cultures showing transient and stable expressions respectively. Addition of novel glycosylation reduced rhEPO secretion dramatically while deletion mutants had little effect except some double deletion mutants (△24/83 and △38/83) and triple mutant (△24/38/83). This fact suggests that not single but combination of changes in glycosyl groups affect secretion of rhEPO in cell culture, possibly via changes in their conformations.

When paprika is cultivated during summer season, thrips starts to occur in the early growing season and reaches its peak in late June. In the treatment using Orius laevigatus and Neoseiulus cucumeris, natural enemies to thrips, thrips density was 87.6/trap and 51.31trap on June 23. After Orius laevigatus and Neoseiulus cucumeris were treated twice on June 24 and July 23, the number decreased considerably. On August 23, it was 36.9/trap and 40.3/trap and from the mid August to early September thrips density remained low. Effect of Orius laevigatus and Neoseiulus cucumeris on suppression of thrips density was 35.7% and 44.6% on July 22, however, the number went up to 88.8% and 80.5% on September 3.

Investigation on oriental melon was carried out for 30 minutes starting at 7 PM every day from March 21 to May 24 to find out the effect of light emitting diode on seedling quality, grafting, growth and flowering of oriental melon. According to the result of the investigation, plant height was longer in Blue, Infrared, Red+Blue and Red treatment and leaf number was higher in Blue, Red+Blue and Infrared treatment than those of control. No big difference was identified between control and Yellow, Green, Ultraviolet treatments. Grafting rate was high in Green, Red+Blue and Green treatment. The number of flower evef}' week in control was nine, the number was almost 1 higher in White and Ultraviolet A treatments, but it was 1 to 4 lower in the rest of treatments. The number of female flowers of control was 10, however, it was 21 in Infrared treatment, 17 in White, 15 in Ultraviolet, 13 in Red+lnfrared, 12 in Blue and Red+Blue, 11 in Yellow and 8 in Green.