The electrochemical analysis of silver ion was performed using cyclic voltammetry (CV) and square-wave (SW) stripping voltammetry, and electrode cell systems were fabricated with graphite pencil electrode (GE) of working, reference and counter electrodes. Also electrolyte was the use of sea water as electrolyte solutions instead of ionic controlled solutions. The optimum analytical conditions for the cyclic and stripping parameters were determined using GE. The results approached the microgram working ranges of SW(ug/L) and CV(ug/L) Ag, and the optimum conditions were applied to frog's tissue and the food samples.

Diagnosis with an ex-vivo gold sensor was done using a modified fluorine-doping sensor, and cyclic voltammetry (CV) redox potentials of 0.4 V anodic and -0.2 V cathodic were obtained. Both peak currents were optimized using square-wave (SW) stripping voltammetry, and an analytical working range of 10-80 ug/L SW was attained. The precision of the 10-mg/L Au was 0.765 (n=8) RSD under the optimum conditions, and the analytical detection limit approached 0.006 ug/L (S/N=3) with only a 60 sec accumulation time. The developed method was used to examine the mouse droppings for medicinal diagnosis.

A investigation of electrochemical analysis of antibiotics Neomycin (C23H46N6O13) was searched using electrochemical square wave (SW) stripping and cyclic voltammetry (CV) using working sensor of the modified carbon nanotube combination electrodes, optimum diagnostic parameters were searched by anodic stripping, final conditions were attained to working range of 1.0-14.0 ng/L, detection limit (S/N) was found to be 0.6 ng/L. The developed method was discovered to be fitting in quality control in the food, pharmaceutical and other manufacturing sectors.

Coriandrum sativum L., an annual herbaceous plant of Apiaceae family. The present study evaluated the anti-oxidant activities and anti-inflammatory effects of ethanol extracts of C. sativum. The anti-oxidant activities of C. sativum were measured by total contents of polyphenol, flavonoid, DPPH and ABTS radical scavenging and reducing power activity. And anti-inflammatory effects of C. sativum were measured by LPS-induced RAW 264.7 cells. The results showed that the contents of total polyphenol and flavonoid were 76.03 ± 1.36 mg of gallic acid equivalents/g and 182.23 ± 4.32 mg of rutin equivalents/g at concentration 1 mg/ mL of C. sativum. The DPPH radical scavenging activity was found to be 52.8% at 500 μg/mL. The ABTS radical scavenging activity was shown in 58.3% after exposure to 1,000 μg/mL. Reducing power activity was found to be 66.8% at 2,000 μg/mL. The inhibitory effect of NO production was found to be 65% concentration 500 μg/mL. In the generation quantity of inflammatory cytokines such as TNF-α and IL-1β in cell culture medium, the expression levels of inflammatory proteins in cells were showed decrease with the increase of concentration. Therefore, we suggest that the C. sativum should be a potential source of alternative anti-inflammatory drug with good anti-inflammatory effects.

To minimize secondary damages from marine environment pollution resulting from marine accidents, International Maritime Organization(IMO) adopted “Guidelines on Places of Refuge(POR) for ships in need of assistance” as Resolution A.949(23) in it is 23rd General Assembly in 2003 and recommends Parties to the Organization designate PoR. This resolution suggests that they establish a “Decision-Making process” so as to provide reasonable PoR when disabled ships request use of PoR. Korea has not been performed introduce a PoR system in the country. Therefore, there is no decision-making process to deal with ships which need PoR. When Korea implements the PoR system in the future, the nation should establish a Decision-Making process to provide reasonable PoR in case ships in need of assistance request for PoR. In order to present what should be considered in the process of establishing a Decision-Making process for PoR in Korea in the future, in this study presents matters which should be considered in the process of founding PoR Decision-Making processes. When Korea tries to conduct POR system so that other countries’ PoR Decision-Making process and the relating process of IMO and REMPEC (Regional Marine Pollution Emergency Response Centre for the Mediterranean Sea) were investigated and analyzed. In addition, in times of marine accident in Korea’s sea areas, Korea’s action manuals for marine accident which regulates management for an accident is analyzed and articles will be presented to be taken into account during establishing final PoR Decision-Making process.

This study was carried out to investigate the effects of wormwood (Artemisia sp.) addition on the growth performance, nutrients utilization and abdominal fat deposition of broiler chickens. Two hundred twenty five and two-day old Arboracre strain male commercial broiler chicks were distributed to 5 treatments with wormwood supplementation levels; C(0%), T₁ (1%), T₂ (3%), T₃ (5%), and T₄ (10%) and with 3 replications each with 5 birds for five weeks. Body weight gain during the experiment was improved in T₁ (1723.0g) compared with that of T₃ (1557.7g) and T₄ (1450.7g) (P＜0.05). Feed intake was significantly (P＜0.05) increased as the levels of wormwood addition increased (C: 2653.8g, T₁: 2852.0, T₂: 2900.3, T₃: 2900.7g, T₄: 2954.7g). Feed conversion rate (feed/gain) was significantly (P＜0.05) increased as the levels of wormwood addition increased (C: 1.55, T₁: 1.66, T₂: 1.70, T₃: 1.86, T₄: 2.04). The days reaching to 2.0㎏ of body weight were expected to be 43.2 days in control, whereas those of group T₁ were reduced to 42.6 days by 0.6 day from control. Nutrient utilization and abdominal fat deposition in the experiment were significantly decreased (P＜0.05) and small intestine contents in the broilers was significantly increased (P＜0.05) as the levels of wormwood addition increased. Therefore, although there was no significant improvement for the performance of broiler chickens with the dietary supplementation of wormwood meal, less than 1% addition of wormwood to broiler diets might have beneficial for human health by reducing the abdominal fat deposition of the broiler chickens.

This study was carried out to investigate inhibitory effect of extracts from Artemisia capillaris Thumb. on maltase, sucrase, α-amylase, nonspecific α-glucosidase, and postprandial hyperglycemia. Methanol extract and organic solvent (n-hexane, ethyl acetate, butanol, aqueous) fractions from the medicinal herb were determined for the inhibitory activities against maltase, sucrase and α-amylase. The methanol extract from A. capillaris strongly inhibited maltase (57%) and α-glucosidase (72%) at the concentration of 100 μg/ml. Among the four fractions (n-hexane, ethyl acetate, butanol, aqueous) examined, the butanol fraction from A. capillaris showed potent inhibitory effects on maltase (73%), sucrase (33%), and α-amylase (75%) at the concentration of 100 μg/ml. The butanol fraction from Artemisia capillaris also exhibited significant reductions (20%) of blood glucose elevation in mice loaded with maltose. These results suggest that the extract from Artemisia capillaris can be used as a new nutraceutical for inhibition on postprandial hyperglycemia

The purpose of this study is to evaluate an efficacy of in vitro differentiated human embryonic stem (hES, MB03) cells expressing Nurr1 in relief of symptomatic motor behavior of Parkinson's disease (PD) animal models MB03 was genetically modified to express Nurr1 protein and was induced to differentiate according to 2-/4+ protocol using retinoic acid and ascorbic acid. The differentiation-induced cells were selected for 10 to 20 days thereafter in N2 medium. Upon selection, cells expressing GFAP, TH, or NF200 were 38.8%, 11%, and 20.5%, respectively. in order to examine therapeutic effects of the differentiated cells in PD animal model, rats were unilaterally lesioned by administration of 6-kydroxydopamine HCI (6-OHDA) into medial forebrain region (MFB, AP -4.4 mm, ML 1.2 mm, DV 78 mm with incision bar set at -2.4 mm), as a reference to bregma and the surface of the skull. Confirmation of successful lesion by apomorphine-induced rotational behavior, differentiated cells were transplanted into the striatum (AP 1.0, ML 3.5, DV -5.0; AP 0.6, ML 2.5, DV -4.5). Improvements of asymmetric motor behavior by the transplantation were examined every two weeks after the surgery. In two weeks, numbers of rotation by the experimental rats were (P<0.05) of the number before transplantation, however, the ratio increased slightly to in six weeks. In contrast, the ratio of sham-grafted animals ranged from 112.3+8.5% to 139.2+28.9% during the examination. Immunohistochemical studies further confirmed the presence, survival, migration, and expression of TH of the transplanted human cells.

Main strategy for a treatment of Parkinson's disease (PD), due to a progressive degeneration of dopaminergic neurons, is a pharmaceutical supplement of dopamine derivatives or ceil replacement therapy. Both of these protocols have pros and cons; former exhibiting a dramatic relief but causing a severe side effects on long-term prescription and latter also having a proven effectiveness but having availability and ethical problems Embryonic stem (ES) cells have several characteristics suitable for this purpose. To investigate a possibility of using ES cells as a carrier of therapeutic gene(s), human ES (hES, MB03) cells were transfected with cDNAs coding for tyrosine hydroxylase (TH) in pcDNA3.1 (+) and the transfectants were selected using neomycin (250 ). Expression of TH being confirmed, two of the positive clone (MBTH2 & 8) were second transfected with GTP cyclohydrolase 1 (GTPCH 1) in pcDNA3.1 (+)-hyg followed by selection with hygromycin-B (150 ) and RT-PCR confirmation. By immune-cytochemistry, these genetically modified but undifferentiated dual drug-resistant cells were found to express few of the neuronal markers, such as NF200, -tubulin, and MAP2 as well as astroglial marker GFAP. This results suggest that over-production of BH4 by ectopically expressed GTPCH I may be involved in the induction of those markers. Transplantation of the cells into striatum of 6-OHDA- denervated PD animal model relieved symptomatic rotational behaviors of the animals. Immunohistochemical analyses showed the presence of human cells within the striatum of the recipients. These results suggest a possibility of using hES cells as a carrier of therapeutic gene(s).