Nickel is widely used in industrial fields such as electrocatalysis and energy storage devices. Although micron-sized nickel particles exhibit excellent mechanical durability, their low specific surface area limits their reactivity. We modified the surface of micron-sized nickel particles with nanostructured nickel oxalate and investigated the effects of the solvent dielectric constant, surfactant, and thermal treatment atmosphere on the resulting particle morphology and phase transformation. Rietveld refinement analysis confirmed that changes in the solvent dielectric constant led to increased or diminished crystallinity of specific planes in nickel oxalate, resulting in diffraction patterns distinct from standard JCPDS data. These structural changes were also found to influence the morphology of the synthesized nickel oxalate. The results demonstrate that nickel oxalate serves as an effective precursor for producing Ni and NiO phases, and shape control of the final product can increase the surface reactivity of micron-sized nickel materials.

Molecular markers have been widely applied in population genetics, diagnostic taxonomy, and genetic mapping, and they can also be used for classifying varieties of Italian ryegrass during field selection. In this study, genome-wide sequence information was generated for 10 Italian ryegrass cultivars (40 samples), including ‘Kowinearly’ (KW), using next-generation sequencing (NGS). Single nucleotide polymorphism (SNP) analysis revealed that only three SNP loci were sufficient to distinguish KW from the other cultivars. Furthermore, 21 alternative barcode sets, each consisting of three SNPs, were identified. These SNP barcode sets provide a reliable criterion for cultivar discrimination in Italian ryegrass and can contribute to the protection of domestic varieties and the advancement of the forage industry in Korea. More broadly, the development of distinguishing markers across Italian ryegrass cultivars will enhance genetic resource identification and support the breeding of high-quality new varieties.

This study reports the development of a new alfalfa (Medicago sativa L.) variety, ‘Alfaone’, at the Forage Production Systems Division, National Institute of Animal Science, Rural Development Administration, Korea, from 2015 to 2023. The variety originated from an artificial cross between Xun Lu (maternal parent) and RadarⅡ Brand (paternal parent), followed by pedigree selection and performance testing. The elite line ‘MsCB01’ was subsequently released as ‘Alfaone’. Regional adaptability trials were conducted for two years (2022–2023) across four representative sites in Korea (Cheonan, Pyeongchang, Jeongeup, and Jinju) to evaluate agronomic traits, forage yield, and quality. Evaluated characteristics included plant height, regrowth ability, winter survival, and lodging resistance. The average dry matter yield of ‘Alfaone’ was 20,811 kg/ha, approximately by about 3% higher than that of the standard cultivar ‘Vernal’ (20,236 kg/ha). Yield superiority was particularly evident in Pyeongchang, suggesting excellent cold tolerance and winter hardiness. Assessment of forage nutritive traits indicated that ‘Alfaone’ was comparable to ‘Vernal’, demonstrating that its yield advantage did not come at the expense of quality. Overall, ‘Alfaone’ is a promising cultivar that combines high productivity with strong adaptability to unfavorable environments, particularly cold-prone regions. Its release is expected to promote the expansion of alfalfa cultivation, enhance forage self-sufficiency, and reduce dependence on imported hay in Korea.

This study evaluated the growth characteristics, forage productivity, and feed value of three Italian ryegrass (Lolium multiflorum Lam.) cultivars ‘Earlybird’, ‘Green call’, and ‘Greenfarm2ho’ over two consecutive growing seasons. Growth traits such as cold tolerance, lodging resistance, disease resistance, and insect resistance were assessed, along with plant height. Forage yield was measured as both fresh and dry matter yields, and feed value was analyzed in terms of CP, NDF, ADF, DMI, DDM, RFV and CA content. Among the tested cultivars, ‘Earlybird’ demonstrated superior performance in several key traits. It showed the greatest lodging resistance and plant height, along with the highest fresh and dry matter yields, although differences in yield were not statistically significant. In terms of feed quality, ‘Earlybird’ had the highest CP (10.8%), lowest NDF (54.7%), and highest RFV (109), indicating excellent palatability and digestibility. ‘Greenfarm2ho’ showed intermediate values across all parameters, suggesting balanced productivity and quality. In contrast, ‘Green call’ showed relatively lower lodging resistance and feed value. Overall, ‘Earlybird’ was identified as a promising cultivar for forage production in temperate climates, combining stable yield performance with high nutritional value. These findings offer valuable guidance for cultivar selection in livestock forage systems aiming to improve both productivity and feed efficiency.

This study was conducted from 2022 to 2024 at the Grassland and Forage Crops Division, National Institute of Animal Science (RDA), in Cheonan, Korea, to develop a medium-maturing variety of Italian ryegrass (Lolium multiflorum Lam.). The newly developed tetraploid cultivar, named ‘Spider’, is characterized by its green leaves, semi-erect growth habit in late autumn, and erect growth habit in mid-spring. With a heading date of May 16, ‘Spider’ is classified as a medium-maturing variety. Compared to the control cultivar ‘Kowinmaster’, ‘Spider’ has a 1.0 mm wider leaf blade, a 1.6 cm longer leaf blade, and is 5 cm taller in plant height. Its dry matter yield (10,169 kg/ha) is significantly higher than that of ‘Kowinmaster’ (p<0.05). The crude protein content of ‘Spider’ is 10.4%, which is 0.2% higher than that of the control. Additionally, ‘Spider’ has a neutral detergent fiber (NDF) content of 49.5% and an acid detergent fiber (ADF) content of 26.6%, showing a 2.2% lower NDF and a 0.2% higher ADF compared to ‘Kowinearly’.

The commercial feed additive, native rumen microbes (RC), derived from a diverse microbial community isolated from the rumen of Hanwoo steers is being explored to enhance rumen fermentation and improve ruminant feed utilization. This study evaluated the impact of native rumen microbes supplementation on methane emissions, microbial diversity, and fermentation efficiency on in vitro assessment. Treatments were as follows: CON (basal diet, without RC); T1 (basal diet + 0.1% RC); T2 (basal diet + 0.2% RC). Rumen fermentation parameters, total gas, and methane production were assessed at 12, 24, and 48 h of incubations. The in vitro gas production was carried out using the Ankom RF Gas Production System. Supplementation of RC significantly reduced the total gas production at 12, 24, and 48 hours of incubation (p < 0.05). Volatile fatty acid concentrations were increased, while acetate and propionate were decreased (p < 0.05) at 48 h by the supplementation of RC. Notably, the 0.1% inclusion level of RC significantly reduced methane production by 28.30% and 21.21% at 12 and 24 hours. Furthermore, microbial diversity analysis revealed significant shifts (p < 0.05) in bacterial composition between the control and treatment groups, while supplementation also promoted the growth of bacterial populations, such as Succiniclasticum. These findings suggest that native rumen microbes supplementation, particularly at 0.1% inclusion level, can enhance rumen microbial composition while significantly reducing methane production in vitro.