알스트로메리아(Alstroemeria)는 알스트로메리아과(Alstroemeriaceae) 에 속하는 단자엽 식물로, 세계적으로 인기 있는 절 화 작물이다. 국내에서도 재배되는 대부분의 품종은 수입묘로, 비싼 로열티를 지불하고 있어 국내 품종 육성의 필요성이 제기 되고 있다. 따라서 본 연구는 수정 전 장벽 타파 방법 중 하나인 화주절단수분법을 알스트로메리아에 적용하여 자가수분을 통 한 착과율과 종자 결실 수를 파악하여 화주절단수분법의 가능성 을 알아보기 위해 수행하였다. 그 결과 착과율은 무 절단, 20% 절단, 50% 절단, 90% 절단, 100% 절단에서 각각 80%, 60%, 30%, 10%, 0%로 나타났으며, 처리구별로 수분 전과 수확 후 자방의 크기는 통계적으로 유의한 차이가 없었다. 종자 결실 수는 무 절단, 20% 절단, 50% 절단, 90% 절단, 100% 절단에서 각각 22개, 10개, 3개, 1개, 0개로 나타났다. 자방 당 종자수는 2.2개 1개 0.2개, 0.1개, 0개로 확인되었다. 이 실험결과를 바탕 으로, 자가수분을 통한 화주절단수분법으로는 자방 당 종자 수 의 증대를 기대하기 어렵지만, 자가불화합성 계통 또는 종간교 배를 통한 품종 육성 시 화주절단수분법이 새로운 품종 육성에 활용될 가능성이 있을 것으로 판단된다.
In this study, silk sericin solutions and films were prepared with different solvents: formic acid and water. Also, silk sericin film from aqueous solution was prepared at different casting temperature. Regardless of solvent type, silk sericin solutions showed a shear thinning implying highly molecular entangled state of silk sericin. Silk sericin aqueous solution showed a higher turbidity than that of formic acid solution. Silk sericin aqueous solution showed shorter gelation time than formic acid. FTIR results showed β-sheet crystallization of silk sericin was affected by casting solvent and temperature. Silk sericin film from aqueous solution showed more β-sheet conformation as the casting temperature was decreased. Silk sericin film from formic acid showed higher crystallinity index than silk sericin films cast from aqueous solution. XRD diffraction measurement showed similar results to those of FTIR. In case of mechanical properties, tensile strength of sericin film from formic acid was higher than sericin films from aqueous solution.
The purpose of this study was to determine the effects of heat appli˗ cation on the immune activities of the human body. To exam, further˗ more, the immune effect from the healthy volunteer(male:15, female:15) by monitoring changes of immune substances such as various leukocytes[total white blood cell(WBC), eosinophil, neutrophil, basophil, monocyte, and lymphocyte], a comparative study with warm water immersion(40.8±0.3℃) and infrared(250W) was carried out. The plasma analysis showed that the count of white blood cell, eosinophil, and neutrophil were elevated in warm water immersion- or infrared˗ stimulated group compared with control group. However, the count of basophil was decreased in both warm water immersion- and infrared-stimulated group than control group. Therefore, these results suggest that the thermostimulation improved immune activity.