낭충봉아부패병에 저항성인 신품종 토종벌을 농업과학원에서 시험재료로 제공받아 일반 농가에서 사육중인 일반벌과 비교하였다. 꿀벌 수밀력을 조사한 결과 일벌 개체당 수밀력은 비저항성 계통보다 저항성계통이 15% 높았으며, 화분채집량은 저항성계통에서는 358mg, 비저항성 계통은 330.3mg으로 저항성계통이 비저항성계통보다 8.4%의 높은 화분채집력을 보였다. 질병저항성을 알아보기 위해 봉개유충 청소행동 평가에서 8시간 후 저항성계통에 서는 51.3%, 비저항성계통에서는 50.7%이었으며, 24시간 후에는 두계통 모두 98.7~100%의 사충 제거율을 보였다. 봉군발육조사에서는 저항성 계통의 경우, 일벌, 유충, 번데기의 수가 비저항성계통에 비하여 41%, 7%, 73% 로 발육이 우수하였다.

본 조사지인 돌리네 습지보호지역은 경북 문경시 산북면 굴봉산 정상부에 위치하였으며, 물이 고이기 힘든 돌리네(doline) 지형에 형성된 희귀 습지이다. 뿐만 아니라 멸종위기종과 법적보호종이 분포하는 등 생물다양성 또한 매우 풍부한 것으로 평가되고 있다. 그러나 습지보호지역 주변으로 과수원 등 경작지가 형성되어 있어, 관리 및 보전을 위한 조사 및 대책이 필요하다. 따라서 본 조사에서는 습지보호지역의 체계적인 관리를 위하여 돌리네 습지의 곤충상을 조사하였다. 조사 방법에는 곤충의 다양한 습성과 서식환경을 고려하여, Searching, Sweeping, Pitfall trap, Bucket trap으로 총 4가지의 채집방법으로 조사를 실시하였다. 그 결과 총 11목 82과 188속 237종이 확인되었으며, 이중 딱정벌레목이 가장 많이 출현하였다. 특이사항으로 환경부지정종 51종(중복포함)이 조사되었으며, 그중 멸종위 기종 1종이 확인되었다. 본 연구를 통해 돌리네 습지보호지역의 곤충상을 파악하고 관리 및 보전에 대한 기초자료를 제공하고자 한다.

Exosomes are Nano-sized lipid vesicles secreted from mammalian cells containing diverse cellular materials such as proteins, lipids, and nucleotides. Multiple lines of evidence indicate that in saliva, exosomes and their contents such as microRNAs (miRNAs) mediate numerous cellular responses upon delivery to recipient cells. The objective of this study was to characterize the different expression profile of exosomal miRNAs in saliva samples, periodically isolated from a single periodontitis patient. Unstimulated saliva was collected from a single patient over time periods for managing periodontitis. MicroRNAs extracted from each phase were investigated for the expression of exosomal miRNAs. Salivary exosomal miRNAs were analyzed using Affymetrix miRNA arrays and prediction of target genes and pathways for its different expression performed using DIANA-mirPath, a web-based, computational tool. Following the delivery of miRNA mimics (hsa-miR-4487, -4532, and -7108-5p) into human gingival fibroblasts, the expression of pro-inflammatory cytokines and activation of the MAPK pathway were evaluated through RT-PCR and western blotting. In each phase, 13 and 43 miRNAs were found to be differently expressed (|FC| ≥ 2). Among these, hsa-miR-4487 (|FC|=9.292005) and hasmiR- 4532 (|FC|=18.322697) were highly up-regulated in the clinically severe phase, whereas hsa-miR-7108-5p (|FC|= 12.20601) was strongly up-regulated in the clinically mild phase. In addition, the overexpression of miRNA mimics in human gingival fibroblasts resulted in a significant induction of IL-6 mRNA expression and p38 phosphorylation. The findings of this study established alterations in salivary exosomal miRNAs which are dependent on the severity of periodontitis and may act as potential candidates for the treatment of oral inflammatory diseases.

Two dimensional(2D) crystals, composed of a single layer or a few atomic layers extracted from layered materials are attracting researchers’ interest due to promising applications in the nanoelectromechanical systems. Worldwide researchers are preparing devices with suspended 2D materials to study their physical and electrical properties. However, during the fabrication process of 2D flakes on a target substrate, contamination occurs, which makes the measurement data less reliable. We propose a dry transfer method using poly-methyl methacrylate(PMMA) for the 2D flakes to transfer onto the targeted substrate. The PMMA is then removed from the device by an N-Methyl-2-pyrrolidone solution and a critical point dryer, which makes the suspended 2D flakes residue free. Our method provides a clean, reliable and controllable way of fabricating micrometer-sized suspended 2D nanosheets.

Mobile shopping motivations affects the interaction between mobile shoppers and mobile retailers. This study examines how mobile shopping motivations affect value co-creation, customer equity drivers, and customer lifetime value through a structural equation model. Mobile shopping motivations as mobile shoppers’ needs are time saving, right purchase and money saving. To meet mobile shoppers’ needs, mobile shoppers, mobile retailers, and other customers are willing to collaborate. Value co-creation that Yi and Gong (2013) scaled includes customer participation behaviour such as information seeking, information sharing, responsible behaviour, and personal interaction, and customer citizenship behaviour such as feedback, helping, advocacy, and tolerance. The results indicate that mobile shopping motivations are significant determinants of value co-creation behaviours, implying that mobile shopping motivations are driving factors of value co-creation. Customer participation behaviour has significant effects on value equity and brand equity while customer citizenship behaviour shows positive effects on brand equity and relationship. As for customer lifetime value, relationship equity has significant positive effect, while value and brand equity had no significant influence. This study also shows that mobile shopping motivations affect both value equity and relationship equity of mobile shopping apps by improving information sharing, responsible behaviour, and personal interaction, feedback, helping, and advocacy. Value equity and relationship equity also have significant effects on customer lifetime value. The authors discuss the theoretical and managerial implications for their findings.