In our previous study, active-single compound (ASC), inhibitor of melanogenesis in B16F10 melanoma cell, was identified from Ganoderma lucidum. Thus, the aim of this study is to analyze the change of the ASC contents in G. lucidum when lacquer tree (LT) was added in cultivation of G. lucidum. In HPLC analysis, significantly increased ASC peak was observed in G. lucidum extract supplemented with 15% (v/v) of LT. In addition, melanogenesis inhibitory effect of G. lucidum extract supplemented with 15% of LT significantly increased when compared to G. lucidum extract without LT supplementation. Furthermore, LT supplementation increased mycelial growth of G. lucidum in both solid and liquid cultivation. These results suggest that is useful as natural ingredient for increasing bioactivity including skin-whitening effect and mycelial growth of G. lucidum.

In our previous study, active-single compound (ASC), inhibitor of melanogenesis in B16F10 melanoma cell, was identified from Ganoderma lucidum. Thus, the aim of this study is to analyze the change of the ASC contents in G. lucidum when oriental raisin tree (ORT) was added in cultivation of G. lucidum. In HPLC analysis, significantly increased ASC peak was observed in G. lucidum extract supplemented with 15% (v/v) of ORT. In addition, melanogenesis inhibitory effect of G. lucidum extract supplemented with 15% of ORT significantly increased when compared to G. lucidum extract without ORT supplementation. Furthermore, ORT supplementation increased mycelial growth of G. lucidum in both solid and liquid cultivation. These results suggest that oriental raisin tree is useful as natural ingredient for increasing bioactivity including skin-whitening effect and mycelial growth of G. lucidum.

The purpose of this experiment is to measure the change in the growth rate and the amount of bioactive compounds when additives are added to mushrooms. According to the additive content, Ganoderma lucidum ASI7004, Ganoderma lucidum ASI7071, Ganoderma. meredithae KACC42868 and Ganoderma lucidum ASI7013 mycelial growth rate variation analysis, oriental raisin tree, Siberian ginseng, chestnut shell, apple pomace, and Korean cabbage additive increased mycelial growth by approximately 2.5 times when compared to the control group(MCM medium). The results of analyzing the total amount of polyphenol in mycelia of G. lucidum based on different kinds of additives have shown that the amount of polyphenol increased only in G. lucidum ASI7004 with 2% Korean cabbage additive. However, when other additives are added, the amount of polyphenol turned out to be lower than original G. lucidum and other individual additives. The results of analyzing the total amount of triterpenoid in mycelia of G. lucidum based on different kinds of additives have shown that the amount of triterpenoid increased in G. lucidum mycelia with apple pomace and Korean cabbage additives. Siberian ginseng additive was effective in increasing the amount of triterpenoid only when 4% Siberian ginseng was added to G. lucidum ASI7004, yet the increased amount was not significant. Although the addition of additives turned out to be effective to the growth rate, it was not effective enough towards the amount of bioactive compounds. Further experiments are required.