Infertility of the pig is directly affects on economic loss and failure of the embryo transfer. In the present case report, we show one rare case of porcine infertility resulted from oviductal obstruction. A gilt showing normal heat behaviors was selected as a recipient of embryo transfer. During the laparotomy surgery, abnormality of the reproductive tract was founded. Several large sized cyst-like structures were founded on infundibulum and body of uterus. Severe enlargement of oviduct represents that obstruction of the oviduct. Sign of fibrosis on the surface of uterus and other internal organs revealed that the obstruction was come arise from prior peritonitis. Mild neutropenia and elevated number of monocytes, eosinophils and platelets in blood smear represent that the peritonitis might be due to chronic parasitic infection. Ovarian function was seems to be normal due to blood progesterone concentration was higher than basal level. The pig was culled because she cannot be recovered by surgical or hormonal treatment.

Early cleavage is a reliable prognostic tool for successful embryo transfer in assisted reproduction because early cleaved embryo show better pregnancy rate after transfer. There for, preparation of good embryo recipient is important factor to optimize efficiency of pig cloning. The present study was performed to evaluate the effect of early cleavage on the in vivo development of cloned embryos and to analyze breed, parity and estrous synchrony to optimize recipient for pig cloning. In vitro matured porcine oocytes derived from local slaughterhouse and fibroblasts derived from miniature pig fetuses were used for somatic cell nuclear transfer (SCNT). Reconstructed embryos were transferred to recipient pigs on the same day of SCNT or after 1~2 days of in vitro culture for selecting early cleaved embryos. Breed, parity and date of standing estrous of recipients were recorded for analysis. After 25~35 days after embryo transfer pregnancy was diagnosed using ultrasonography, and pregnant recipients were monitored till delivery. Between purebred and crossbred, no significant difference was founded in both pregnancy and delivery rates. However, early cleaved embryos showed significantly higher pregnancy (46.2%) and delivery (12.8%) rates compared to non-selectively transferred group (24.8% and 4.5%, respectively). The results also showed that the recipients showing standing estrous on the same day of SCNT and less than 4 parities were most suitable for pig cloning.

This study was performed to evaluate the effectiveness of food commodity supplementary program for low-income, living alone elderly people. The subjects were 57 low income aged females who lived alone in Bucheon city. The food commodity supplementary program lasted for 6 months, supplying one meal per day consisting with grains, meat, vegetables, dairy and fruit. Dietary intake, anthropometric measurements, and blood profiles were assessed. After 6 months of food commodity supplemented period, dietary intakes of protein(p〈0.05), calcium(p〈0.05), iron(p〈0.05) and vitamin B(p〈0.001) were significantly increased. The mean weight(p〈0.05) and BMI(p〈0.05) were increased, and the frequency of BMI over 25kg/m2 was also raised from 54.4% to 59.7%. Serum concentrations of total protein(p〈0.001) and albumin(p〈0.001) were significantly increased, indicating improvement of protein status. The frequency of anemia was decreased from 45.6% to 26.3%. However, the proportion of dyslipidemia was increased. In general the stable food commodity supplementary program was effective to improve general nutritional status of the poor aged who lived alone, however, nutrient intakes still did not meet RDA for the elderly and the rates of obesity and dyslipidemia were increased. Therefore, local government should provide more active food support program and the selection of food items for commodity should be evaluated at regular intervals.