The Lentinula edodes is the third most widely produced mushroom in the world. It is a rich source of proteins, fats, carbohydrates, fiber, vitamins, minerals and β -glucan. The aim of the this study was to evaluate the physicochemical characteristics changes such as proximate composition, minerals, and contents of β -glucan according to the different product area. And we evaluated the antioxidant properties of L. edodes. Also, we determined the standardization of manufacture process for commercial products using L. edodes. Eleven L. edodes were cultivated in oak, and samples were collected by different product area. And the L. edodes cultivated in sawdust have been producted in Jangheung. All samples are shade dried and those dried samples were used for this test.

Electrical treatment has been widely used for porcine oocytes activation. However, developmental rates following electrical activation of porcine oocytes is relatively inefficient compared to other domestic animals. To investigate the effects of porcine oocytes on combined activation by both chemical and electrical treatment, in-vitro matured oocytes were activated by combined cycloheximide and electrical pulses treatment. Cumulus-free oocytes were exposed with NCSU-23 medium containing cycloheximide (10μgml) for 0, 5, 10, 20, 30 min and then activated by electrical pulse treatment and cultured in PZM-3 for 8 days. Also effects of exposure to 6.25μM calcium ionophore for 2 min for cumulus-free oocytes were tested. The percentage of blastocyst formation in 10 min exposure to 10μgml cycloheximide and electrical pulse treatment was significantly increased (P<0.05) than in the control group. And exposure to 6.25μM calcium ionophore for 2 min with 10μgml cycloheximide for 10min and electrical pulse treatment significantly increased (P<0.05) the percentage of blastocyst developmental rates than the control group. In conclusion, activation by combined cycloheximide and electrical stimulation treatment promoted the subsequent development of porcine oocytes and improved the subsequence blastocyst development