Clithon retropictus has been protected by the Ministry of Environment as an endangered species since 1998 and has been listed on the state red list of endangered species category II. It is viewed as a representative for all endangered species in the northeastern coastal waters of Korea. Most of the habitats of C. retropictus have been found to be in an unstable state because of development projects such as road construction, small stream development, irrigation for securing agricultural water, and flood prevention. These habitats are damaged by small stream maintenance projects and development, and the risk of damage is increasing and active efforts are needed in order to protect them. Although the Ministry of Environment is striving to preserve this endangered species, the habitat of C. retropictus is still facing external threats because it spreads to only a small area at high densities. Therefore, in order to protect the habitat of C. retropictus, a level II endangered species, it is urgent to make an effort to minimize habitat damage and to take measures for its protection.

This study investigated in the vascular plants of high value ecological conservation Unjangsan (Mt.), Jeollabuk-do, Korea. The numbers of vascular plants were summarized as 604 taxa including 107 families, 343 genera, 530 species, 5 subspecies, 58 varieties and 11 forms. The rare plants were 12 taxa including Paeonia japonica (VU), Syringa patula var. kamibayshii (LC), Cynanchum inamoenum (VU), Schpolia japonica (LC), Scrophularia koraiensis (DD) and so on. The Korean endemic plants were 16 taxa including Pseudostellaria coreana, Clematis trichotoma, Stewartia pseudocamellia, Ajuga spectabilis, Cirsium setidens and so on. In the specific plants by floristic region were 60 taxa, a degree I were 29 taxa (Sisymbrium leteum, Ilex macropoda, Eleutherococcus sessiliflorus, Cirsium pendulum, Veratrum versicolor and so on), 21 taxa of a degree II (Morus cathayana, Phellodendron amurense, Euonymus pauciflorus, Angelica tenuissima, Heloniopsis koreana and so on), 8 taxa of a degree III (Rhynchosia acuminatifolia, Stewartia pseudocamellia, Cynanchum inamoenum, Schpolia japonica, Scrophularia koraiensis and so on), Wisteria floribunda (Planted) of a degree IV and Prunus yedoensis (Planted) of a degree V. The naturalized plants were 42 taxa including Phytolacca americana, Solanum carolinense, Ambrosia artemisiifolia, Aster pilosus, Poa pratensis and so on. Naturalization rate (NR) was 6.9% of all 604 taxa of vascular plants and the invasive alien plants were 3 taxa including Solanum carolinense, Ambrosia artemisiifolia, Aster pilosus. This study is to identify the main plant distribution and protecting valuable plant populations Unjangsan areas in Jeollabuk-do is located in the inland performing will want to take as the basis for designating an ecological and landscape conservation areas.

The purpose of this study is to construct the basic data for establishing the forest ecosystem and environment by studying the flora distributed in Mt. Paljae, Chungcheongnam-do. The number of flora were summarized as 292 taxa including 88 families, 214 genera, 258 species, 2 subspecies, 27 varieties and 5 forms. The rare plant was Melothria japonica. The Korean endemic plants were 3 taxa including Salix koriyanagi, Philadelphus schrenkii and Paulownia coreana. The specific plants by floristic region were 16 taxa including Betula davurica, Salix chaenomeloides, Dictamnus dasycarpus, Hosta capitata and so forth. The naturalized plants were 34 taxa including Fallopica dumetorum, Rumex obtusifolius, Ailanthus altissima, Festuca arundinacea and so forth. Most of the plants commonly originated from North America. The 5 naturalized degree that was widely distributed and had many individual was the most common. Until 1921, after the opening of 1 period was the most common in the introduced period. The invasive alien plants were 4 taxa including Rumex acetosella, Solanum carolinense, Aster pilosus and Paspalum distichum var. indutum.

Tyrophagus putrescentiae (Tp) as a storage mite inhabitats in stored grains, hay, and straw at agricultural areas. T. putrescentiae stimulates an immune response and triggers inflammatory cytokines release, and thus it is a source of allergen that sensitize and induce allergic reactions. Also, T. putrescentiae has been reported to cause asthma and atopic disease by cross-reactivity with Dermatophagoides pteronyssinus (Dp). The study on T. putrescentiae in human monocytic THP-1 cells is not enough to understand cytokine expression and pathological mechanisms. The aim of this study is to investigate the effect of T. putrescentiae extract (TpE) on production of inflammatory cytokines and expression of mRNA level in THP-1 cells. THP-1 cells are treated with TpE and supernatants were analyzed for the production of cytokines using enzyme-linked immunosorbent assay (ELISA). mRNA level in the culture cells was measured by a reverse transcriptase-polymerase chain reaction (RT-PCR). As a result of this study, TpE significantly induced secretion of interleukin-6, interleukin-8, and monocyte chemotactic protein-1 (MCP-1) in THP-1 cells in time- and dose-dependent manner. These results suggest that TpE may play a role in contributing to inflammatory disease through stimulation of immune cell. Further research of T. putrescentiae is needed to understand the elucidation of the pathogenic mechanism.

홀스타인 암소 육성우에 옥수수사일리지와 볏짚을 급여하면서 영양수준을 80, 100 그리고 12%(NRC 요구량 대비)로 급여할때 육성우의 중체량 및 영양소 섭취량을 조사하기 위하여 육성우 30두를 각 처리당 5두씩 완전임의 배치로 공시히여 체중 400kg까지 시험을 수행한 결과는 다음과 같다. 1. 옥수수사일리지 및 볏짚급여시 육성기 최대 일당중체량은 각각 체중 200과 250kg 일때였다. 2. 체중이 증가할수록 DM, CP 및 TDN 요구량은 증가되