Members of the Vps (Vacuolar protein sorting) protein family involved in the formation of the retromer complex have been discovered in a variety of species such as yeast, mouse, and human. A mammalian retromer complex is composed of Vps26, Vps29, and Vps35 proteins and plays and important role in cation-independent mannose-6-phosphate receptor retrieval from the endosome to the trans-Golgi network. In this study, we have identified the full-length sequences of the retromer components of Vps26, Vps29, and Vps35 in micro pigs. The cDNA sequences of these retromer components have been determined and the result showed there is 99% homology among the component counterparts from mouse, micro pigs, and humans. In addition, the retromer complexes formed with hetero-components were found in the brain of micro pigs. Based on above results, we suggest mammalian Vps components are well conserved in micro pigs.

This study selects Sunwol local group, Gusang local group and Yongjun local group to examine the appropriate amount of applied fertilizer in cultivating the double crop seed of Alisma plantago by transplantation in the southern area, improve and complements the double cropping techniques and contribute to stable production. While the plant height is 45,47 and 49cm and the number of leaves is 11.7, 12.5 and 14.4 at non-fertilizing plot, the plant height is 67, 72 and 75cm and the number of leaves is 15.8, 17.2 and 19.3 at all fertilizing plot and their growth is active and in the character of plant height and the number of leaves, especially in the groove of N-P2O5-K2O= 30-15-45kg/10a, the mean plant height of Sunwol is 75cm and mean number of its leaves is 19.3, the mean plant height and number of leaves in Gusang are 72cm and 17.2 respectively and those of Yongjun are 67cm and 15.8 respectively. While the number of floral axis is 1,2 in non-fertilizing plot, that of the whole varieties in all fertilizing plot is 3, 4, 5 and the mean floral axis of Sunwol is N-P2O5-K2O = 10-5-15, 20-10-30, 30-15-45 kg/10a and 18-0-18 of complex fertilizer and 21-17-17kg/10a and it is small in all fertilizing plot. The yield of dry root per 10a is high in all fertilizing grooves and especially in the groove of N-P2O5-K2O = 30-15-45 kg/10a, it is 372.6kg, in the groove of 18-0-18kg/10a using complex fertilizer, it is 389.1kg and in that of 21-17-17kg/10a, it is 376.7kg.6.7kg.

The result of this experiment which are conducted, to improve the cultivation technology of Alisma plantago, to increase its quantity and to contribute for stable production with Yongiun local group by examining the optimal planting density and transplanting period of double cropping of Alisma plantago in the southern region. The characters of plant height, leaf width and length tend to be reduced as the seeding period is later by the order of the 10th, 20th and 30th of July. The period required for flowering is reduced as the transplanting period is later and dense planting is applied. Plant height, the number of leaves and yield of dry root have much quantity at the dense planting density of 20× 15cm as they are transplanted later in the 30th of August or the 10th of September, but they are rather less in sparse planting density of 20×25cm or 20×35cm.