This study was conducted to investigate the seasonal occurrence of Phyllocnistis citrella Stainton (Citrus leafminer; CLM) and parasitoids in Jeju region, where the damage of CLM to the citrus farm has been recently increased. For this study, annual CLM mornitoring using sex pheromone traps were conducted in the citrus farm of Jeju from 2011 to 2015. The mean annual occurrence of CLS was 477,389 and appeared to increase along the temperature.

The genus Phyllocnistis belongs to the family Gracillariidae including 85 described species in the world (De Prins and De Prins 2005). The genus has been placed under the family Phyllocnistidae, which is now the subfamily of the family Gracillariidae. In Korea, only one species of the family, Phyllocnistis citrella Stainton has been listed for the first time by Ko (1969) without the taxonomic information. It was first reported by Heppner (1993) from North America, and it has become to the major pest of Rutaceae, especially damaging on the genus Citrus spp. It has been called as CLM (citrus leafminer) in the world. This study was carried out to identify and monitor CLM easily and correctly with providing the genitalic structures and DNA barcode which is now one of the major insect pest on citrus farm.

Cucumber is a typical monoecious plant with individual male and female flowers, and sex expression in cucumber is mainly determined by three major genes: F/f, M/m and A/a. Gynoecy plays an important role in cucumber hybrid breeding and use of gynoecious lines as maternal parent ensures high productivity. The purpose of this study is to identify a co-dominant molecular marker linked to F locus to distinguish homozygous and heterozygous gynoecious plants for cucumber breeding programme. Firstly, we analyzed the sequence polymorphism of 5 gynoecious and 5 monoecious inbred lines to detect polymorphism to develop the marker linked to F locus. A pair of specific primer based on insertion/deletion polymorphism on branched-chain amino acid transaminase (BCAT) gene was designed and examined the polymorphism in the parents, F1 and F2 segregating population derived from gynoecious (WJEF11) and monoecious (WNEF8) inbred lines. The result showed that the specific fragment amplified with Cs-Female-F/Cs-Female-R, was identified as a co-dominant marker and co-segregated with sex phenotype in F2 population. Furthermore, we present a new linkage map for F locus using Indel markers. This is the first report for the development of F locus specific co-dominant marker which can distinguish homozygous and heterozygous gynoecious and it could be used in marker-assisted selection in cucumber breeding.

Downy mildew, caused by P. cubensis, is one of the most devastating diseases in cucumber (Cucumis sativus) worldwide. Due to the variation and mutation of the races of P. cubensis, host resistance in cucumber has been lost in recent years, so the identification of new sources of resistance is one of the most important targets in cucumber breeding programs. Moderate levels of resistance against downy mildew has been identified in different cucumber varieties. In this study, we identified new downy 2mildew resistance QTLs in cucumber using F2 mapping populations originated from the hybridization between breeding lines of cucumber. We used both classical QTL mapping based on SSR markers and GBS (gentyping based sequencing) based QTL mapping. In this presentation, detailed information about downy mildew resistance related QTL will be presented

Camelina is a promising energy crop for the biodiesel industry, especially for production of airplane fuel. In addition, its distinctive fatty acid profile and high protein contents satisfy the nutraceutical and animal feed uses. About 200 accession collected from all of the world were selected and evaluated during 2012, 2013 and 2014. The important agricultural traits were detemined by field and greenhouse conditions. In addition, genetic diversity was assessed by GBS approach. a total of 20 Camelina spp. (mostly C. sativa) accessions collected in different locations in Europe and showed different agronomic traits are sequenced. In this section, detail GBS analysis of camelina will be presented.