변전소 구조물 내부에 설치되는 전력설비의 내진설계 시 사용되는 변수인 가속도 증폭계수는 미국, 일본과 국내의 변전소 내진설 계기준에서 제시되어 있다. 국내 설계기준에 제시된 가속도 증폭계수는 미국, 일본의 설계기준에서 제시된 계수와는 달리 변전소 구조물의 층수가 4층 이상일 경우에는 동적해석을 수행하여 가속도 증폭계수를 구하게 되어 있다. 국내의 변전소 구조물은 대부분 층수가 4~5층이므로 기존의 가속도 증폭계수는 실제 변전소 구조물에 적용하기에 미흡한 상황이다. 국내 변전소 구조물 형식에 적합한 가속도 증폭계수를 제시하기 위하여 대표적인 7가지 구조형식의 변전소 구조물에 대하여 가속도 증폭계수를 평가하였다. 가속도증폭 계수는 변전소 구조물에 대하여 원거리 지진과 근거리 지진을 사용하여 내부-구조물 응답스펙트럼을 작성하여 이로부터 평가하였다. 미국, 일본 전력설비 내진설계 기준에 따른 각각의 가속도 증폭계수 αj, αA는 근거리 및 원거리 지진을 사용한 동적 해석으로 구한 가 속도 증폭계수에 비하여 다소 과대평가하는 경향이 있다.

Artificial insemination and embryo transfer is one of the most important factors affecting to the production of fawn from deer nuclear transfer in the field of deer farms. This study* was conducted to establish the production technology of nuclear transfered embryo in deer. For estrus synchronization or superovulation tretments in flower deer and elk, each 10 does were inserted into the vagina for 14 days with CIDR (Pfizer New Zealand Ltd., NZ) for elk and Ring-CIDR (Bioculture Co., Ltd., Korea) for flower deer, and then those inserted devices were removed. The estrus synchronization of each 6 does were induced by the intramuscular injection of PGF2α (25 mg/head) and PG600 (hCG 200IU + PMSG 400IU, Intevet, Holland). Then, the superovulation of each 4 does of flower deer and elk was induced by additional injection of FSH (200 mg/ head) twice with an interval of 24 hours , respectively. Follicular oocytes were collected from each 2 does superovulated after 48 hours since the injection of PG600 and FSH. In the meantime, the ovarian response and the number of the collected ovarian follicles were investigated with the surgical operations. As a result, the average number of the collected ovarian follicles were 8.5 and 9.0 in flower deer and elk, respectively. The ovarian follicles collected from each two does were cultured in vitro for 48 hours with m-DMEM medium, and then the cell fusion was carried out after the nuclear transfer by the antler cell. As a result, 5 out of 18 ovarian follicles collected from 2 elk does were reached on the MII stage, but there was no generation resulting from the nuclear transferred embryos by the antler cell after enucleation. In 2 flower does, 7 out of 17 ovarian follicles were reached to the MII stage, but one of them was developed to parthenogenetic embryo as well despite a case of fusion from the nuclear transferred embryo. Embryos were collected in a surgical way on the 7th day after artificial insemination, numbers of average embryos collected were 2.5 and 3.0 in each 2 flower deer and elk does superovulated, respectively. The collected two embryos were transplanted to each 2 does synchronized. As a result, a head of fawn was produced from only one elk doe, where as a head of fawn were delivered from one out of 4 elk does artificial inseminated. Given these findings, we consider that more or less of problems might have occurred in vitro culture system of ovarian follicles in the production of nuclear transfered deer embryos. In addition, the greatest reason why both the aetificial insemination and embryo transfer failed was considered attributable to stress due to anesthesia and catching.