As a member of fungus gnats, the genus Bradysia can be the pests on many kind of agricultural crops, including mushroom farming. Feeding on healthy roots of crops, they can also spread root rot diseases. In Korea Bradysia difformis is known as major pest in greenhouse crops (carrot, cucumbers, garlic, melon, potatoes, radish, strawberries, sugarbeet, sweetpepper, sweetpotato, etc). Besides Bradysia difformis, we report six Bradysia species for the first time in Korea.

In the family Sciaridae, only 3 species have been recorded in Korea, as the pests on various crops and mushrooms in glasshouse (Bradysia difformis Frey, 1948, Bradysia procera (Winnertz, 1868), Lycoriella ingenua (Dufour, 1839)), even though Sciaridae is one of the species-rich families in the order Diptera. There are plenty of species unknown in Korea which are very hard to identify by morphological characters in the larval, pupal stages and also female adults as well. Therefore, using the sequences of cytochrome oxidase I gene (COI) from adult male, we tested the utilities of DNA barcode to identify the species of sciarid flies.

Five genera and 7 species of sciarid flies were collected in shiitake mushroom farm from 2007 to 2009. Among them, Lycoriella ingenua was dominant as a possible pest of the shiitake mushroom cultivated in plastic bottles. Other six species are reported for the first time in Korea.

Camptomyia is a genus of gall midges belonging to subfamily Porricondilinae. On the study of shiitake mushroom farm in Korea, three species of maggots were collected. Among them, two Camptomyia spp. were recognized as the major pests. Colonies of these maggots were feeding on the mycelium of shiitake mushroom, disturbing the formation of mushroom fruit body. The morphology of C. heterobia and C. corticalis were illustrated with the description.

Camptomyia is a genus of gall midges belonging to subfamily Porricondilinae, with 42 species known from the palaearctic region. In November 2007, serious damages of the Shiitake mushroom logs were reported in many shiitake farms from the middle part of South Korea. Rearing the adults in laboratory, a Camptomyia species was recognized as the major species causing damages on shiitake. Colonies of maggots were observed, feeding the mycelium of shiitake mushroom and the phloem of oak timbers, disturbing the mushroom fruit body formation. The morphology of the larvae and adult of this Camptomyia heterobia. is illustrated with the description of the damage symptom.

A total of 12 genera, 37 species in the subfamily Eumeninae was recognized from the insect collection of several universities and institute in Korea (Seoul National University, Kangwon National University, National institute of Agricultural Science & Tech.). In 2007, 13 species in 9 genera were collected throughout the country by sweeping net and bamboo nest traps. Among them, Anterhynchium flavomarginatum koreanum (86 specimens), Discoelius zonalis (29 specimens), and Euodynerus dantici (20 specimens) were most dominant. One species (Stenodynerus sp.) was confirmed as new to science. Using the bamboo nest traps, 7 species of Eumenid wasps were collected and the larvae of Gelechids and Pyralids were confirmed as the prey of Discoelius zonalis and Anterhynchium flavomarginatum koreanum, respectively.