The genus Testudobracon (Braconidae: Braconinae) is reported for the first tine in Korea. The genus is a small genuswith 12 species distributed in the world, of which species are known as ectoparasitoids of the cecidomyiid (Diptera).Testudobracon species are distinguished from other braconid genus by the following characters: terminal flagellomere pointedbut without a prominent spine; mesoscutum largely setae, notauli well developed.In this study, a braconid genus, Testudobracon, is recorded from Korea for the firs time based on Testudobracon pleuralis(Ashmead, 1906). we provided diagnosis, host list and photographs of diagnostic characteristics for this species.

Aphidiinae in Braconidae includes more than 400 species within approximately 60 genera worldwide. Species of Aphidiinae are mostly known as solitary koinobiont endoparasitoids specifically associated with aphid hosts, and some of them are used for biological control. In the present study, two aphiniine species are newly recognized from South Korea based on morphological and molecular study: Aphidius rhopalosiphi de Stefani-Perez 1902 and A. uzbekistanicus Luzhetzki 1960. Although It was very difficult to discriminate two species morphologically, we cleary identified them by using COI (cytochrome c oxidase subunit I) DNA Barcode sequences. Here we present their morphological descriptions, illustrations and barcoding results.

Many species of braconids play an important role in controlling the populations of insects in agricultural and natural ecosystems, which parasitize pests. Nevertheless, the family Braconidae was scarcely research and DNA barcoding of Braconidae has not been studied in Korea. DNA barcoding can rapidly perform species identification, and classify difficult species in morphological identification. Because most braconids are very small and cryptic in morphology, DNA barcoding is useful in Braconidae taxonomy. In this study, genomic DNA of 280 individual samples were extracted, and, among them, 208 samples were obtained COI (cytochrome c oxidase I) barcode sequences. As a consequence of blast, 48 samples were identified in genus-level, and 51 sample were identified in species-level. Among them, 4 species were revealed as unrecorded in Korea.

The family Scelionidae is distributed all around the world, which has approximately 3,000 species within 150 genera in the world. To date 39 species of 15 genera from 3 subfamilies in Scelionidae are recorded in South Korea. In this study, we report a new record of the genus Idiris with one species. There are many parasitory and predatory insects specializing on spider eggs. Among scelionids, some species (Baeus achaearneus, Comastichus zopheros, Idris sp.) parasitize spider eggs. However, there was no Korean record for scelionid wasps parasitizing in egg sacs of spider. Based on morphology, ecology, and DNA identification, we recognized this species, Idris sp., being new to Korea. All examined specimens and DNA samples are deposited in Kunsan National University.

The genus Toxares (Braconidae: Aphidiinae) is reported for the first time in Korea. Members of Toxares are known as solitary koinobiont endoparasitoids especially specific for aphidine aphids. In this study, Toxares deltiger (Haliday, 1833) is found as new to Korea, which has a wide host range for 31 aphid species within Aphidinae. Thus, this species would be useful as natural enemy for biological control in the future. Diagnosis and host information are included.