Background : The ginseng cool-season and semi-shade plant undergoes photosynthesis smoothly at low temperature in the morning but its efficiency falls at high temperature in the afternoon. Therefore, if it is possible to control light transmission rates (LTR) every day, it will be able to draw an ideal result in the ginseng growth by attracting a lot of lights before 11am and attracting few lights after 11am which is the affect point of the photosynthetic efficiency. Methods and Results : While installing the sun shading facility that could control the light environment based on 11am and surveying the ginseng's growth and photosynthetic characteristics. The photosynthetic efficiency increased 2.81 times compared to conventional practices without the change of the chlorophyll content although LTR was high because of the low temperature in the morning. The aboveground part growth of the ginseng which adapted to the light environment increased to influence the root weight, when the ginseng was 4 years old, the fresh root weight was averagely 33.3g per plant which increased by 81% than 18.4g of conventional practices. Conclusion : If the light environment management according with the photosynthetic nature of the ginseng is conducted based on 11am, it will be possible to produce a large capacity and excellent-quality ginseng.

Background : Ginseng is a semi-shade plant which prefers cool temperature and mild lighting, and artificially installed shading facility is required from preventing photo-oxidation occurrence when ginseng is exposed to direct sunlight. High temperature damages are commonly noticed due to recent global warming although more farmers are using aluminum foil and blue light-proof screen instead of 4-layered polyethylene shade net (blue 3 + black 1) to prevent the rise in temperature and influx of rainwater, there are insufficient research conducted. Methods and Results : In mid-March, 2015, we have installed steel A type shading facility after transplanting 2-year-old domestic variety ginseng in 7 lines, 10 rows per 3.3㎡. We have prevented the excessive influx of direct light by fixing 4-layered shade net, aluminum foil and blue light-proof screen as shading materials on facilities and adding 2-layered black polyethylene shade net. During July and August with high temperature, light transmission rates have shown varying according to shading materials used: blue light-proof screen (8.4%) > aluminum foil (5.9%) > 4-layered shade net (3.3%). In case of the highest temperature reached, the order followed blue light-proof screen (42.3℃) > 4-layered shade net (40.8℃) > aluminum foil (38.3℃), and in case of average temperature it followed blue light-proof screen (48.7%) > 4-layered shade net (37.6%) > aluminum foil (33.2%). Although there were no difference about leaf area among the shading materials, ginseng leaf grown in aluminum foil has lower chlorophyll content than the other two treatment plot because of photooxidation by light damage. The 3-yr-old ginseng root weight harvested in end of October increased as follows light-proof screen (14.4g) > 4-layered shade net (11.9g) > aluminum foil (9.8g). Conclusion : Aluminum foil showed greater influx of light but lower temperature than 4-layered shade net due to its materialistic character. However the front side of foil can reflect light and cause photo-oxidation of rear-planted ginseng leaves, so it is a need for improvement. In case of blue light-proof screen, although it had higher inside temperature, the root weight of ginseng was the heaviest because of less influx of light and destruction of chlorophyll.

Background : Most ginseng seedlings are cultivated in conventional shading facility. However, due to recent climate changes and soil-related bacterial issues, the production of healthy ginseng seedling with its rhizome weigh more than 0.8g has greatly dwindled. Hence, growing seedling system that raises ginseng on nursery soil in greenhouse is being more highlighted. Nevertheless, there are no sufficient researches conducted to study the growth stability after transplantation of ginseng seedling grown at nursery (GSGN). Methods and Results : After categorizing GSGN produced in 2014 according to individual weight (0.5-0.6g, 0.6-0.7g, 0.7-0.8g, 0.8-0.9g), those GSGN with 0.8-0.9g conventional ginseng seedling (CGS) have been transplanted on preparation field in 17, March of the following year. It has turned out that there were no significant differences about seedling emergence rate and weights between GSGN and CGS both same seedling weight of 0.8-0.9g in 25, June after 100 days transplantation. The 2-year-old ginseng growth of transplanted GSGN according to weight has shown strongly positive correlation between the weight of ginseng seedling and their growth. Especially the leaf area and weight have shown significant correlations represented as Y=212.15-567.32X+499.5X2(R2=0.9988) and Y=4.9-12.9X+11.17X2(R2=0.9788), respectively. Meanwhile, there was no significant correlation noticed treatment plot in relationship between seedling emergence rate and chlorophyll content. Conclusion : With the same root weight of GSGN and CGS, it can be judged that the growth of the 2-year-old GSGN is stable.

Carotenoids are major secondary compounds in Citrus determining the color of fruit and nutritional values. Carotenoids are isoprenoic compounds, and function as color pigments in the flower and fruit to attract pollinators and seed-dispersing animal and chromophore for light harvest and photoprotectant during photosynthesis. In the aim of developing new cultivars with high value using molecular breeding technology, we had performed screening of flesh and peel specific genes by differentially expressed gene screening in Citrus unshiu fruits. From the screening, carotenoid isomerase (CRTISO)1, which converts pro-lycopene to all-trans-lycopene, was identified as peel-specifically expressed gene. In this study, the gene encoding the CRTISO1 was cloned, sequenced, and compared to the CRTISOs in other plant species. Comparison of the cds sequence to other plant species revealed 75% and 78% identity with CRTISO1 of Zea maize and CRTISO2 of Arabidopsis thaliana respectively. We also cloned CRTISO2 from C. unshiu which declines the expression while maturation (Kato et al., 2004), and the gene structure was analyzed. This is the first work reporting the full sequence and gene structure of CRTISOs in C. unshiu, and would give important information in understanding the carotenoid synthesis in the Citrus fruit.