A number of plating companies have been exposed to the risk of fire due to unexpected temperature increasing of water or other reasons in a plating bath. Since the companies are not able to forecast the unexpected temperature increasing of plating bath and most of raw materials in the bath have low ignition temperature, it is easy to be exposed to the risk of fire. Thus, in previous study, we tried to monitor and notice the dangerous change of temperature of water immediately to prevent the risk of fire from plating process. However, unfortunately previous studies were not able to shut out the fundamental cause of fire since bath temperature sensor can detect air temperature when the level sensor was malfunctioned. In this paper we developed the Teflon heater which contains a built in temperature sensor and improved plating equipment system. Teflon heater is improved using Pt 100Ω sensor which can detect until 600°C . When the bath temperature sensor detects over 60°C or the Teflon heater sensor detects over 240°C they temporarily shut down the heater to control temperature. Also relay completely shuts down main power when detects instant temperature is detected over 5% of 240°C by the heater sensor to prevent teflon melting down and fire spreads. Developed plating equipment system can monitor a real time temperature in the teflon tube and bath water. Therefore we think the proposed plating equipment can eliminate the possibility of fire in plating processes fundamentally.

In this paper we developed the Teflon heater which contains a built in temperature sensor and a relay for improved plating equipment system. Teflon heater is improved by using Pt 100Ω sensor which can detect until 600°C. When the bath temperature sensor detects over 60°C or the Teflon heater sensor detects over 240°C they temporarily shut down the heater to control temperature. Also relay completely shuts down main power when detects instant temperature is detected over 5% of 240°C by the heater sensor to prevent teflon melting down and fire spreads. Developed plating equipment system can monitor a real time temperature in the teflon tube and bath water. Therefore we think the proposed plating equipment can eliminate the possibility of fire in plating processes fundamentally.

The aim of this study was to evaluate the effects of different straw volume (0.5 and 5.0 ㎖) on cryopreservation in boar semen. Semen quality was evaluated the motility, viability, abnormality, acrosome intactness and membrane integrity. And there were also examined the development rates of IVM/IVF embryos using frozen-thawed boar semen. Boar semen were frozen until 5℃ for 3 hours using cell freezer and making the straws, and then freezing by lowing the straws into styrofoam box on the 8 cm above the LN2. In different straw volume (0.5 and 5.0 ㎖), sperm viability and abnormality were not differ in 0.5 and 5.0 ㎖ straws, but sperm motility were significantly higher in 0.5 ㎖ straws (61.3%) than in 5.0 ㎖ straws (56.3%) (p<0.05). In the Coomassie Brillient Blue (CBB), Hoechst 33258/Propidium Iodide (H258/PI) staining and Hypoosmotic Swelling Test (HOST), the acrosome intactness and sperm membrane integrity were not differ in 0.5 and 5.0 ㎖ straws, but sperm survival rate was significantly higher in 0.5 ㎖ straw (65.0%) than in 5.0 ㎖l straw (55.0%) (p<0.05). Employing the Chlortetracycline (CTC)/Hoechst33258 (H258), all treatment group were not differ in characteristic of uncapacitated acrosome-intact sperm (F), capacitated and acrosome-intact sperm (B-type) and acrosomereaction seprm (AR-type). In the developmental rate of IVM/IVF embryos using frozen-thawed boar semen in different straw volumes, the developmental rate of morula plus blastocysts were 19.9% in 0.5 ㎖ straw and 18.8% in 5.0 ㎖ straw, respectively. These results indicated that straw volume affects the semen motility and sperm survival rate, but not other semen characteristic and developmental rate of IVM/IVF embryos.