‘Neugeunsuperjami’, a late maturing and blackish purple pigmented rice cultivar, was developed from a cross between Gumjeongbyeo and Hwaseonchalbyeo. During selected by pedigree breeding method until F8 generation, a promising line, SR28757-22-2-4-2-1-1-2-B, was selected and advanced by late maturing and content of Cyanidin 3-glucoside. Advance line designated as the name of ‘KNOU 13’. This variety headed on August 28, 140 days to heading after sowing. The culm length and the panicle length of ‘Neugeunsuperjami’ was 91.0 cm and 20.0 cm, respectively. The number of panicle per hill was 10.5 and the number of grain per panicle was 88.6. The dehulling recovery of ‘Neugeunsuperjami’ was about 80.5% and 1,000-grain weight was about 19.7 g, heavier than that of ‘Heugjinju’. ‘Neugeunsuperjami’ has 3~5 times higher Cyanidin-3-glucoside(C3G) content compared with ‘Heugjinju’.

‘Pareunsuperjami’, a early maturing and blackish purple pigmented rice cultivar, was developed from a cross between Heugjinju and Suwon 425. During selected by pedigree breeding method until F8 generation, a promising line, C3GM(CG2-2-70-1-1-3-2-B), was selected and advanced by early maturing and contents of cyanidin 3-glucoside. Advance line designated as the name of 'KNOU 3'. This variety headed on July. 30, 115 days to heading after sowing. The culm length and the panicle length of 'Pareunsuperjami' was 63.5cm and 21.3cm, respectively. The number of panicle per hill was 9.4 and the number of grain per panicles was 98.0. The ratio of fertility of ' Pareunsuperjami’ was about 80.5% and 1,000-grain weight was about 18.8 g, heavier than that of ‘Heugjinju’. ' Pareunsuperjami ' has 3~4 times higher Cyanidine-3-glucoside(C3G) contents compared with ‘Heugjinju’.

전통적인 교배육종을 통하여 천연색소(C3G) 함량을 높인 ‘슈퍼자미2호’(흑진주벼/수원 425호//대립벼 1호) 품종의 작물학적 특성과 품질특성을 조사 분석한 중부평야지에서 평균 출수기는 8월 30일로 만생종이며, 간장은 106 cm 정도이며 임실률은 82.0%였다. 또한 현미천립중은 30.1 g 정도로 ‘슈퍼자미’(26.2g)보다 무거운 품종이다. ‘슈퍼자미2호’ 품종의 현미 장폭비는 2.08의 장원형으로 흑진주벼에 비해 길이와 폭이 12%, 26%로 증가된 대립종이다. 미량원소 중 K, Ca 함량은 ‘흑진주벼’에 비해 낮았고, 단백질, 회분 함량도 낮게, 열량, 지방, 탄수화물 함량은 비슷한 수준으로 나타났다. ‘슈퍼자미2호’ 품종의 C3G 색소함량은 2013년에는 1,782 mg(100 g 종자), 2014년에는 1,980 mg(100 g 종자)으로서 ‘슈퍼자미’ 보다는 다소 낮았으나 ‘흑진주벼’ 보다는 9배 이상 높았다.

The rice sucrose synthase 3 (RSUS3) localized predominantly inrice seed endosperm may play an important role in the starch filling in the milky stage of rice seed. As the genetic diversity at this locus is not known yet, forty three rice varieties/accessions were objected to amplify full sequence of the RSUS3 to examine the distribution of DNA polymorphisms. A total of 254 sequence variants, including 82, 150 and 22SNP sand indels, were successfully identified in whole length of 7,733bp sequence comprising promoter, exon and intron, and 3’ down stream non transcribed region(NTR). Eleven haplotypes were distinguishable among 43 rice varieties based on the nucleotide variation on the three defined regions (5’NTR, transcript and 3’NTR). The promoter region showed the occurrence of a base change on a cis-element which might involve a functional role of the motif in seed-specific expression. Non random process seemed to be acted in the genetic diversity of RSUS3geneamongricegermplasmusedinthisstudy. The analysis of polymorphism sites indicated a history of eleven minimum recombination mostly occurred in the transcribed region. This result might provide an insight for a clasditic approach for establishing future genetic association studies of RSUS3locus.

Sucrose synthase 3 which is a third active gene present in rice, is localized predominantly in rice endosperm. This sucrose synthase 3 may play an important role in the starch filling in the milky stage rice seed, probably involving in the starch physicochemical properties. As the genetic diversity at this locus is little informed, forty three rice consisting of japonica, indica and Oryza rufipogon were targeted to amplify full sequence of sucrose synthase 3 to examine the frequency and distribution of nucleotide polymorphism. Total of 755 all sequence variants detected, 491 single nucleotide polymorphisms (SNPs) and 264 indels were successfully identified in 7618 bp of sequence containing the sucrose synthase 3 transcript, promoter and 3' non-transcribed region. The frequency of nucleotide changes and indels were high, on average one polymorphism per 15.5 bp and one indel per 28.9 bp with 11 sequence-based haplotypes distinguishable among the varieties and lines. Both the frequency of nucleotide changes and indels were frequent in non-coding region, but rare in coding region. Sequencing a polymorphism region in the promoter showed one base change on one of cis-element from T (CATGCATA to A (CATGCACA) that might implicate in seed specificity. The presence of a high number of haplotype shared by a few varieties indicated a little information on linkage disequilibrium.