Current studies have revealed the capacity of mesenchymal stem cells (MSCs) in term of immunomodulatory properties, and this distinct potential is downgraded according to the disease duration of patients-derived MSCs. In order to enhance the immunomodulatory and anti-tumorigenic properties of the rheumatoid arthritis (RA) joints-derived MSCs, we aggregate synovial fluid-derived MSCs from RA joints (RA-hMSCs) into 3D-spheroids by the use of hanging drop culture method. Cells were isolated from synovial fluids of RA joints with longstanding active status over 13 years. For aggregation of RA-hMSCs into 3D-spheroids, cells were plated in hanging drops in 30 μL of advanced DMEM (ADMEM) containing 25,000-30,000 cells/ drop and cultured for 48 h. To analyze the comparative immunomodulatory effects of 3D-spheroid and 2D monolayer cultured RA-hMSCs and then cells were cultured in ADMEM supplemented with 20% of synovial fluids of RA patients for 48 h and were evaluated by qRT-PCR for their expression of mRNA levels of inflammatory and antiinflammatory markers. Cellular aggregation of RA-hMSCs was observed and cells were aggregate into a single sphere. Following treatment of RA patient’s synovial fluids into the RA-hMSCs, spheroids formed RA-hMSCs showed significantly (p < 0.05) higher expression of TNFα stimulated gene/protein 6 (TSG-6) than the monolayer cultured RAhMSCs. Therefore, the 3D-spheroid culture methods of RA-hMSCs were more effective than 2D monolayer cultures in suppressing inflammatory response treated with 20% of RA-synovial fluids by expression of TNFα (TSG-6) according to the immune response and enhanced secretion of inflammatory factors.

Treatment of spontaneous retroperitoneal hemorrhage (SRH) is mainly conservative. However, if intra-abdominal hypertension (IAH) or abdominal compartment syndrome (ACS) develops, the treatment strategy should be more aggressive. Surgical decompression has been the gold standard; however, it is very invasive and highly morbid. Thus, percutaneous catheter drainage (PCD) has been introduced as an alternative therapeutic option. We report on a case of successful PCD for prevention of progression of IAH to ACS in a patient with SRH after coronary stent implantation. This case showed that PCD can be an efficacious and safe method for treatment of IAH with impending ACS.

Choline Chloride를 이용하여 고구마의 단작재배와 맥후작재배간에 어떤 수준의 농도에서 괴근의 비대촉진과 생육 및 수량에 미치는 영향을 구명하기 위하여 시험하였든바 그 결과는 다음과 같다. 1) 처리의 적정농도는 재배기에 따라 다소 차이가 있어 단작재배에서는 23.3ppm 그리고 맥후작 재배의 경우 31.1ppm이 적정농도이고 처리시간은 24시간이며 처리방법은 묘를 채취한 후묘의 기부만을 침지하면 좋다. 2) Choline Chloride는 지상부의 생육을 억제하여 만장을 단축케 하고 건물중을 저하케 하였다. 3) 지하부의 생육관계는 지상부의 동화산물을 지하부에 전류케 함으로써 괴근수를 많게 하는 동시에 수확기별 수량의 증가가 현저하였고 그 경향은 적기수학에서 보다도 조기수확에서 높았다. 4) 따라서 일반유기성분의 함유율 분석결과 당분전분등이 높은 편이고 그밖의 조섬유, 조단백질등은 낮은 편으로서 금후 더욱 연구할 필요가 있다고 느끼는 바이다.