The major limiting factor of the reproductive performance on many farms is the failure to detect estrus in a timely and accurate manner. The estrus detection for Hanwoo cows (Bos taurus coreanea) is generally performed by the visual observation which is estimated at about 40% detection rate only. This recurring problem among farmers has motivated the development of various estrus detection aids. The mounting event duration threshold, standing heat event frequency threshold, estrus detection algorithm and so on, are necessarily needed in order to detect the estrus of Hanwoo cows accurately when using some automatic devices. However, they are not yet investigated for Hanwoo cows. Therefore, the objective of this study was to establish a mounting event duration threshold and standing heat event frequency threshold more specifically for Hanwoo breed by discriminating the duration of standing heat mounting from shorter duration spurious mountings which routinely occur in cattle herds. One hundred and fifty Hanwoo cows with an age between 2 to 5 years were used in this study. Cows that were expected to come into estrus within 3days were randomly assigned to each compartment for the estrus group (EG). The heated cows were video recorded for about 24 hours until after post estrus. The results showed that Hanwoo cows can be considered on estrus when it stand immobile during mounted by any other cow in more than or equal to 3.15s and 3.22s in chest-tail head mount (CTM) and head-above back mount (HBM) and that occurs consecutively at least three times within 876.4s interval. The algorithm was also developed using the thresholds of the mount duration, mount interval and consecutive occurrence number.

SERPINB3 (also known Squamous cell carcinoma antigen 1, SCCA1) is involved in apoptosis, immune response, cell migration and invasiveness of cells. It has been investigated in various types of squamous cell carcinoma. Therefore we investigated the functional role of SERPINB3 gene in human epithelial ovarian cancer (EOC) using laying hens, the most relevant animal model. In 136 laying hens, EOC was found in 10 (7.4%). We compared the expression and localization of SERPINB3 using RT-PCR, quantitative RT-PCR, in situ hybridization and immunohistochemistry, and SERPINB3 activation was detected in chicken and human ovarian cancer cell lines using immunofluorescence microscopy. Thereafter, we examined the prognostic value of SERPINB3 expression in patients with EOC by multivariate linear logistic regression and Cox’ proportional hazard analyses. In present study, SERPINB3 mRNA was induced in cancerous ovaries (p< 0.01), and it was only expressed in the glandular epithelium(GE) of cancerous ovaries of laying hens. SERPINB3 protein was localized predominantly to the nucleus of glandular epithelium in cancerous ovaries of laying hens, and it was abundant in the nucleus of both chicken and human ovarian cancer cell lines. In 109 human patients with EOC, 15 (13.8%), 66 (60.6%) and 28 (25.7%) of those patients showed weak, moderate and strong expression of SERPINB3 protein, respectively. Strong expression of SERPINB3 protein was a prognostic factor for platinum resistance (adjusted OR, 5.94; 95% Confidence Limits, 1.21-29.15). Therefore SERPINB3 may play an important role in ovarian carcinogenesis and be a novel biomarker for predicting platinum resistance and a poor prognosis for survival in patients with EOC. This research was funded by the World Class University (WCU) program (R31-10056), Basic Science Research Program (2010- 0013078) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology and by the Next-Generation BioGreen 21 Program (No.PJ008142), Rural Development Administration, Republic of Korea.

1. 전형적인 조숙성 재래종의 특성은 월동전에는 잎이 담녹색 광엽 직입이며 성숙시에는 화모이 짧고 직수이며 입중이 가볍다. 또한 내한성이 약한 재내종도 상기 조숙성 품종과 같은 특성을 지니고 있다. 이는 한숙성과 약한 내한성간에는 밀접한 관계가 있기 때문에 당연한 것으로 생각된다. 2. 강한 내한성과 관련된 특성은 않지 않으나 대체로 월동전에 포복형이거나 반포복형이며 엽색은 녹색 또는 암녹색을 띠며 협엽, 장간, 당숙인 경향이 있다. 2. 강한 내한성과 관련된 특성은 많지 않으나 대체로 월동전에 포복형이거나 반포복형이며 엽색은 녹색 또는 암록색을 띠며 협엽, 장간, 중숙인 경향이 있다. 3. 조숙성 집단에서 내한성이 강한 품종의 선발은 대단히 어렵고, 또한 매년 합리적인 내한성 검정을 실시하기란 극히 곤란하나, 본 내항성과 관련된 형질연구에서 중생인 재래종을 이용하므로서 내한성이 강한 품종이 선발가능성을 보여주고 있다. 4. 중생이며 내한성이 강한 품종과 관련이 있는 것으로 여겨지는 특성은 장망, 장수, 장립, 장간, 늘어진잎, 중간지엽폭 및 장, 중간화경장, 월동전의 반포복형 및 중간엽폭 등이며, 중생이며 내한성이 약한 지엽이 넓고, 월동전에 잎이 직입, 담록색, 광엽 등의 특성이 있다.