The intracellular endosymbiont bacterium Wolbachia is currently considered the most abundant in arthropods and has also been isolated from nematodes, amphipods, isopods, mites and spiders. Recently, Wolbachia-based research was focused on the control of disease vector-population, such as several mosquitoes such as genus Aedes and Anopheles which cause dengue fever and malaria, respectively. For the analysis of regional difference between vector mosquito Aedes albopictus and Wolbachia, we selected different regions and collected Ae. albopictus which were distinguished with mountain chain and waterway. Whole genomic DNA were extracted from collected specimens with 9 regions. PCR analysis and sequencing were accomplished in each specimen for Wolbachia detection and identification using WSP gene. As a results, almost mosquitoes were infected with two strain of Wolbachia both wAlbA and wAlbB. However, regional separation of vector mosquitoes, wAlbA strain of Wolbachia were showed more than 98% sequence similarity. In this study, we first reported that Wolbachia infection and type of Wolbachia in Korea and endosymbiont Wolbachia was showed highly sequence homologies.

Density of malaria vector mosquito was monitored at 24 sites in Korea to estimate malaria epidemic from 2004 to 2009. Twenty four locations include six in Gwangwon province and nine in Kyonggi province and nine in Incheon metropolitan city. Mosquitoes collected by public health centers in 24 sites were sent to each RIPHE(Research Institute of Public Health & Environment) then the RIPHE identified the malaria vector mosquitoes. KCDC analysed the results. Number of malaria vector mosquito collected in 2009 shapely decreased over 33 % when compared with number of mosquito collected from 2004 to 2009. In 2009, malaria vector mosquitoes firstly were collected at Injegun on 2nd week of April (6-13, April) and the highest peak of collected mosquito was demonstrated on 3rd week of August. Number of collected mosquito sharply decreased below 10 at 3rd week of september and None of mosquito was collected 2nd week October. Decrease of number of malaria vector mosquito collected in 2009 seem to be related with lowest temperature around midnight and precipitation days per month.