The green peach aphid, Myzus persicae (Sulzer), is one of the most important insect pests in the world. We have explored the possibility of using sounds as a stressor to control this species. Five sine wave frequencies (100, 500, 1000, 5000, 10000 Hz) with combination of three intensity (66, 78, 90 dB) were used as acoustic stimuli in the playback experiments. For behavioral bioassay, we examined frequencies of honeydew production (HDP) and wagging behavior, and duration of moving behavior. The HDP occurs regularly during each stage of nymphal or mature period, and rate of the HDP indicates a degree of food consumption. Aphids conduct wagging to determine a host plant or to find a feeding site. The moving behavior may be regarded as dispersal. As results, all acoustic stimuli significantly reduced rate of the HDP and increased rate of the moving behavior. There was a significant effect to induce the moving behavior in the test of 5000 Hz. In addition, the acoustic stimuli using 5000 Hz were the most effective to disturb feeding behavior of M. persicae at an individual level.

The green peach aphid, Myzus persicae (Sulzer), is one of the most important insect pests in the world. We have explored the possibility of using sounds as a control agent in this species. Sine waves with four different frequencies (100, 500, 1000, 5000 Hz) were used as acoustic stimuli in the playback experiments. As a behavioral bioassay, we recorded honey dew production (HDP), wagging, and walking. HDP occurs regularly at each stage of nymphal and adult periods, and the rate of HDP may indicate a degree of food consumption. Aphids conduct wagging to determine a host plant or to find a feeding site. Walking may be regarded as dispersal. All acoustic stimuli significantly reduced the rates of HDP and increased the rates of wagging. There were no significant effect of acoustic stimuli on walking. Based on the bioassay, the acoustic stimulus with 5000 Hz seemed to be the most effective on inhibiting feeding behavior of this aphid species at an individual level.

The Green Peach aphid, Myzus persicae Sulzer, is one of the most widespread insect pests worldwide. We established the ethogram of this species during the pre-reproductive period to understand the behavioral repertoire, organization of behaviors, and the context in which they occur. The behaviors that we observed include honeydew production (HDP), walking, wagging, pre-molting and molting. We noted the frequency and the amount of time for each behavior at different nymphal stages. As the nymphal stage increased, the rate of HDP decreased. The HDP seemed to occurred at regular duration and interval in each nymphal stage, while the wagging and walking seemed to be irregular behaviors. When wagging occurred before HDP, the successive HDP was significantly delayed, suggesting that aphids might not feed during wagging. Due to regularity through all nymphal-stages, HPD can be used for a good behavioral indicator in the related bioassay.

The green peach aphid, Myzuspersicae Sulzer, is one of the most important insect pest in the world. To assess the efficiency of sound wave to control aphids, we treated a different combination of intensity (66dB, 78dB, 90dB) and frequency (100Hz, 500Hz, 1000Hz) of acoustic stimulus to the aphids. Then we analyzed aphid’s behaviors occurred in each different combination group of acoustic stimulus and control group, and compared them to confirm the most effective sound. We used honey-dew-production (HDP) for behavioral bioassay, which occurs regularly in each nymphal stage and adult period. The rate of HDP decreased in the whole experimental group when compared with control group (F1,358=79.415,P<0.0001). The most effective acoustic frequency was100Hz in which aphids reduced the rate of HDP to approximately 1/3 times lower than that of control group. The treatments of 1000Hz and 500Hz were effective in the order of appearance. The effect of acoustic stimulus seems irregular in the different intensities, and the highest intensity did not show the strongest effect in each frequency. Consequently, the rate of HDP seemed to be affected by the acoustic frequency more than the intensity.

The green peach aphid, Myzus persicae, is one of the most widespread insect pest worldwide. We documented all behaviors of this species during the pre-reproductive period to understand the behavioral repertoire, organization of behaviors, and the context in which they occur. The behaviors that we observed include honey-dew production, walking, wagging, and molting. Honey-dew production occurred regularly at every 30-60 min throughout all nymphal stages. Walking seemed to occur when the host condition was not good, probably related to searching for a new host. Wagging, which was the turning of abdomen with the stylet in the plant, was also frequently observed, but the function of this behavior was unclear. We also noted the frequency and the amount of time for each behavior at different nymphal stages.

As an environment-friendly phytosanitary measure, CATTS (controlled atmosphere temperature treatment system) has been developed to kill several quarantine insect pests infesting subtropical agricultural commodities. This study tested any possibility to apply CATTS to apples to effectively eliminate the peach fruit moth, Carposina sasakii, which has been regarded as a quarantine insect from the imported countries. When the larvae of C. sasakii were directly exposed to 46℃ (an installed lethal temperature of CATTS), they showed a median lethal time at 14.66 min. Addition of high carbon dioxide to the temperature treatment enhanced the thermal limit susceptibility of C. sasakii to 46℃. The larvae internally infesting apples were tested using this CATTS device and showed 100% lethality after 60 min exposure to a treatment of 46℃ under 15% CO2 in the chamber. This study suggests a possibility that CATTS can be applied as a quarantine measure to kill the larvae of C. sasakii locating inside the apples. To understand the CATTS effect, a heat shock protein was cloned. Hsp90 was partially sequenced and showed its expression in response to heat treatment. CATTS was likely to suppress hsp90 expression.

Mitochondrial genome is inherited in maternal origin without recombination by mating and its specific regions have been used to monitor insect pest populations in agriculture. The oriental fruit moth, Grapholita molesta, is a serious pest on apple industry by its direct damage on fruits. This study reports a full sequence of mitochondrial genome of G. molesta. Sequence contigs were made by primary PCRs on conserved regions and subsequent PCRs to fill the gaps. Annotated genes were highly matched to the sequences of other lepidopteran species. However, a few positions of tRNA genes on the genome were different to other mitochondrial genomes.