Being a rare form of sentence formation, not many languages seem to have Tag question constructions as English does (Culicover 1992:193). Besides, Cuenca (1997:4) notes that the English Tag question construction has received less and less attention in the later period of generative syntax, due to the colloquial characteristics of the construction. Of the two major distinct approaches to English Tag question constructions including Mono-clausal approaches such as Klima (1964), Arbini (1969), and den Dikkien (1995), and bi-clausal approaches such as Huddleston (1969), Culicover (1992), McCawley (1998), and Sailor (2009), the current paper proposes a Minimalist approach to Tag Question Construction using a series of Head movement, Focalization of vP, a Polarity reversing functional projection, PolP, which contains an abstract morpheme [NEG], and adjunction mechanism. Through these thorough and meticulous steps, the English Tag question construction receives a Minimalist facelift.

To successful molecular breeding, identification and functional characterization of breeding related genes and development of molecular breeding techniques using DNA markers are essential. Although the development of a useful marker is difficult in the aspect of time, cost and effort, many markers are being developed to be used in molecular breeding and developed markers have been used in many fields. Single nucleotide polymorphisms (SNPs) markers were widely used for genomic research and breeding, but has hardly been validated for screening functional genes in olive flounder. We identified single nucleotide polymorphisms (SNPs) from expressed sequence tag (EST) database in olive flounder; out of a total 4,327 ESTs, 693 contigs and 514 SNPs were detected in total EST, and these substitutions include 297 transitions and 217 transversions. As a result, 144 SNP markers were developed on the basis of 514 SNP to selection of useful gene region, and then applied to each of eight wild and culture olive flounder (total 16 samples). In our experimental result, only 32 markers had detected polymorphism in sample, also identified 21 transitions and 11 transversions, whereas indel was not detected in polymorphic SNPs. Heterozygosity of wild and cultured olive flounder using the 32 SNP markers is 0.34 and 0.29, respectively. In conclusion, we identified SNP and polymorphism in olive flounder using newly designed marker, it supports that developed markers are suitable for SNP detection and diversity analysis in olive flounder. The outcome of this study can be basic data for researches for immunity gene and characteristic with SNP.

Molecular markers are useful for selecting to include superior character genetic like as strong immune system and rapid growth in fish. The marker is also very important part of breeding technology in Olive flounder (Paralichthys olivaceus). Single nucleotide polymorphisms (SNPs) marker is already in use widely for genomic research and breeding. But this SNPs marker hardly has been validated for screening functional genes in Olive flounder. We study identify single nucleotide polymorphisms (SNPs) on Expressed sequence tag (EST) database, develop usable SNP marker and apply to wild sample and cultured of olive flounder. As a result, Out of total 4.327 ESTs, 693contigs and 514 SNP from total contigs were detected while these substitutions include 297 transitions and 217 transversions. 144 developed markers were applied in 16 samples (wild 8, culture 8), Out of total marker, only 32 markers had detected polymorphic in sample. Polymorphism of 32 markers was observed in the variety genes region involved in immunity and protein synthesis. And the 32 marker were identified 21 transitions, 11 transversions, and indel was not detected in polymorphic SNPs. The analysis on heterozygosity by sample showed 0.34 in wild sample and 0.29 in cultured sample. In conclusion, we was identified SNP and Polymorphism by designed new marker, it supports that development marker is suitable for SNP detection and diversity analysis in Olive flounder. The outcome of this study can be basic data for researches for immunity gene and characteristic with SNP.

This study was performed to analyze genetic relationship of the four major Cucurbitaceae crop. We used 120 Expressed Sequence Tag(EST)-Simple Sequence Repeat(SSR) primer sets of developed from watermelon and published in International Cucurbit Genomics Initiative (ICuGI) database. Among 120 EST-SSR primer, 51(49.17%) EST-SSR primer set successfully amplified and 49(40.8%) EST-SSR primer set showed polymorphisms among eight cultivars of Cucurbitaceae. In the first instance, amplified PCR products analysis was conducted by the agarose-gel electrophoresis then further analyzed by using Fragment Analyzer. A total 382 PCR band were producted by 49 EST-SSR primers in 24 plant panels, used the analysis of pairwise similarity and dendrogram construction. Assessment of the genetic relationships resulted in similarity index with range of 0.0103 to 0.8452. In dendrogram, 24 plant panels were formed three major groups (A, B, C) and 7 subgroups (A-1, A-2, B-1, B-2, B-3, C-1, C-2). Major group A was comprised of 2 subgroups, subgroup A-1 (6 watermelon cultivars, Citrullus lanatus var. vulgaris Schrad.) and subgroup A-2 (3 wild type watermelon, Citrullus lanatus var. citroides Mats. & Nakai). Major group B was comprised of 3 subgroups, subgroup B-1 (4 melon cultivars, Cucumis melo var. cantalupensis Naudin.), subgroup B-2 (2 oriental melon cultivars, Cucumis melo var. conomon Makino.) and subgroup B-3 (5 cucumber cultivars, Cucumis sativus L.). Major group C was comprised of 2 subgroups, subgroup C-1 [2 squash/ pumpkin cultivars, Cucurbita moschata (Duch. ex Lam.)/Duch. & Poir. and Cucurbita maxima Duch.] and subgroup C-2(2 squash/pumpkin cultivars, Cucurbita pepo L./Cucurbita ficifolia Bouche.)

Recently, proteome analysis is becoming a powerful tool for the functional characterization of plants. Due to the availability of vast nucleotide sequence information and based on the progress achieved in sensitive and rapid protein identification by mass spectrometry, proteome approaches open up now perspectives to analyze the complex functions of model crop species at different level. In this study, we have N-terminal sequencing data for the 100 embryo and 53 seed proteins of rice separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) were collected and systematically organized for a protein sequence data-file. An attempt was made to link the embryo proteins of rice to DNA sequences for understanding their functions. One hundred proteins of the 700 spots were detected in the embryo using 2-DE gels whereas we used micro sequenced. Of these, 28% of the embryo proteins were matched to DNA sequences with known functions, but 72% of the proteins were identified to be unknown functions as previously reported by Woo et al.,. In addition, twenty-four spots of protein with 100% of homology and nine with over 80% were matched to ESTs (expressed sequence tags) after expanding the amino acid sequences of the protein spots by Database searches using the available EST databases of rice at the NCBI (http://www/ncbi.nlm.nih.gov/) and DDBJ (http://www.ddbj.nig.ac.jp/). Also, a total of 53 proteins out of 700 protein spots separated on the 2-DE gels were analyzed by the peptide mass fingerprinting method (MALDI-TOF/MS). High-quality mass spectra suitable for peptide mass fingerprinting were obtained from 41 spots. Using the ESI-Q-TOF/MS, however, we were able to identify 53 seed proteins of rice, including 12 proteins not registered in database. The rapid expansion of DNA sequence databases to the utilization of EST now provides the whole or partial gene sequences of model organisms, and the recent advances in protein micro-characterization by mass spectrometry allow the possibility of linking these DNA sequences to the proteins in functional complexes. Proteome Database of rice is updated, and is available on the World Wide Web at http://gene64.rda.affrc.go. This work shows that the proteome analysis could be a useful strategy to link the sequence information to the functional genomics.

Rye (Secale cereale L.) chromatins have been used to introduce agronomically important traits into wheat (Triticum aestivum L.). Wheat-rye translocations in the form of 1RS.1AL, 1RS.1BL, 2BS.2RL have been developed for an important genetic source of disease and pest resistance. The long arm of rye chromosome 2 (2RL) has valuable genes that confer resistance to pests such as biotype L of Hessian fly, powdery mildew, leaf and stem rust. Here, we report the generation and analysis of expressed sequence tags from Hessian fly infested wheat-rye translocation. RNAs were isolated from young seedlings infested by Hessian fly. cDNA library was constructed using Clontech cDNA library construction kit. Random sequencing of candidate clones were performed. The EST clones might be useful to clone target gene sequences and would provide clues on molecular interaction between wheat and Hessian fly.

본 논문은 UHF 대역 RFID 시스템용 고감도 광대역 태그 안테나의 설계에 대하여 기술한다. 제안된 태그 안테나의 크기는 60 mm × 10 mm × 1 mm이다. 공진 주파수는 910 MHz 이고, - 10 dB이하 대역폭은 약 900 MHz이다. 측정된 반사손실과 지향성 패턴은 계산결과 잘 일치하는 것으로 확인되었다. 칩을 가진 제안한 태그 안테나의 인식거리는 6.5 m로 관측되었으며, 이 값은 상용 태그 안테나의 인식거리보다 평균 0.5 m이상 멀리 감지된 결과이다.

이 논문은 차량용 사이드 미러에 적합한 광대역 RFID 태그안테나를 설계하고, 차체에 의한 지향성의 특성을 제시한다. 제안된 태그 안테나는 인식거리와 광대역 특성을 향상시키기 위해 대칭 구조로 설계되었다 제안된 태그 안테나(30 mm×24 mm×1 mm)는 910 MHz에서 공진하며 대역폭은 780 MHz(540 MHz~1320 MHz)를 가진다. 칩 임피던스는 16 - j131 Ω이고, 상용 칩의 복소 공액 임피던스가 태그 안테나의 설계에 사용되었다. 제안된 태그 안테나는 차량의 사이드 미러의 내부에 위치한다. 사이드 미러의 유전율뿐만 아니라 차체(도체)에 대한 태그 안테나의 효과를 평가하기 위해, 지향패턴 측정과 인식거리가 계산되고 측정되었다. 차량용 RFID 시스템을 위한 최적의 위치가 사이드 미러 내부에서 관측되었으며, 계산된 결과들은 측정된 결과들과 잘 일치하였다.

이 논문은 차량 사이드 미러용 고감도 RFID 태그 안테나의 설계에 대해 나타내었다 제안된 태그 안테나(154 mm×66 mm×1;mm)는 900 MHz에서 공진하며 690 MHz(490 MHz~1180 MHz)의 넓은 대역폭을 가진다. 태그 안테나의 측정된 이득과 인식거리는 각각 5.8 dBd와 10 m 이다. 차체를 포함한 사이드미러 내부에 위치한 제안된 태그 안테나의 방사패턴과 인식거리가 전파암실에서 측정되었다. 90˚근방에서의 인식거리는 차체가 없는 제안된 안테나의 인식거리와의 비교로부터 약 1.5 m 확장된 것을 확인하였다. 제안된 태그안테나의 우수한 성능이 상용 태그안테나의 측정된 결과들과의 비교에 의해 관측되고 입증되었다.

본 논문은 910 MHz 대역에서 동작하는 RFID (radio Frequency identification) 태그 안테나의 소형화 설계 기법을 제안한다. folded-dipole 구조와 미앤더 선로 구조를 적용하여 태그 안테나의 소형화 설계를 행하였다. 최대 전력 전달을 위해서 테그 안테나와 칩의 임피던스의 허수부는 공액 정합되었다. 최적화된 안테나의 크기는 50 nm × 40 nm × 1.6 nm로, 참고문헌 [4]와 비교하여 크기가 62 % 줄었다. 제작된 태그 안테나의 측정결과들은 예상과 잘 일치하는 것으로 확인되었다. 칩이 내장된 태그 안테나의 인식거리는 약 5 m로 관측되었다.