To search a new pig pheromonal odorant, the N1-allyl-N2-(tetrahydrofuran-2-ylmethyl)oxalamide molecule predicted by ligand based approach and molecular docking method was synthesized by nucleophilic addition-elimination reaction (AdNU-E) between N-allyloxalamic acid ethylester and tetrahydrofurylmethylamine. According to the evaluation results for efficiency of pig estrus control, the synthesized pig pheromonal N1-allyl-N2-(tetrahydrofuran-2- ylmethyl)oxalamide molecule advanced the estrus by 11.3 days (p<0.05) compared with the non-pheromone group. And from these results, it is predicted that the synthesized pig pheromonal compound will be able to increase the reproduction efficiency of pig.

분자들이 용액 내에서의 cluster나 박막 내에서의 cluster와 같이 다른 cluster로 뭉쳐졌을 때, 가시광선과 자외선부근의 영역에서 fullerene C60의 전자흡수 스펙트럼 변화가 관찰되었다. 2.73eV에서 흡수 피크들이 관찰되었는데, 이러한 피크들은 독립된 분자들로부터 온 것이 아니라 분자들의 직접적인 상호작용으로부터 온 것으로 생각된다. Grained fullerene 박막에서는 흡수피크들이 3.35eV에서도 관찰되었는데 이러한 피크들은 grain의 계면에서 분자들의 상호작용으로부터 온 것으로 생각된다. 이러한 흡수의 Dichroism은 비등방성 macrostructure를 갖는 시료에서도 관찰되었다.

We have compared the column densities of H 2 C O , 13 C O , C 18 O , 12 C O , CS, and H C O + to the visual extinctions derived from star counts in eight dark clouds, L1317, B1, L1551, L1535, L1544, L134, L134N, and B335. We examined the degree of correlation between molecules and extinctions. The contours of 13 C O , H 2 C O , and CS distributions show an excellent coincidence with those of the visual extinction. Even though the plots of molecule brightness temperature or column density as a function of the visual extinction show a considerable scatter, a good correlation is found between the two quantities. The turnover of the ratios of 13 C O and H 2 C O column densities to the visual extinction at high extinctions observed in several clouds is not apparent in this work. The ratios seem to maintain constant values within the range of A V we studied. The slopes of the relations between the molecule column density and extinction, and the threshold values of extinction for detection of molecules are comparable to the other works. The cause of the slope difference is discussed. We derived, for the first time, the relations of CS and H C O + column densities and the visual extinction. The ratio of CS column density to extinction is at least two orders of magnitude lower than the mean value for 13 C O , but the threshold for detection of CS is comparable to that of 13 C O . The ratio of the H C O + column density to extinction is one to two orders of magnitude lower than the mean ratio for H 2 C O .

Previously we have shown that human abdominal adipose derived-stem cells (ADSCs) could aggregate during the high-density culture in the presence of human serum (HS). In the present study, we observed that human cord blood serum (CBS) and follicular fluid (HFF) also induced aggregation. Similarly, porcine serum could induce aggregation whereas bovine and sheep sera induced little aggregation. qRT-PCR analyses demonstrated that, compared to FBS-cultured ADSCs, HScultured cells exhibited higher level of mRNA expression of CLDN3, -6, -7, -15, and -16 genes among the tight junction proteins. ADSCs examined at the time of aggregation by culture with HS, BSA, HFF, CBS, or porcine serum showed significantly higher level of mRNA expression of JAM2 among JAM family members. In contrast, cells cultured in FBS, bovine serum or sheep serum, showed lower level of JAM2 expression. Immunocytochemical analyses demonstrated that the aggregates of HS-cultured cells (HS-Agg) showed intense staining against the anti-JAM2 antibody whereas neither nonaggregated cells (HS-Ex) nor FBS-cultured cells exhibited weak staining. Western blot results showed that HS-Agg expressed JAM2 protein more prominently than HS-Ex and FBS-cultured cells, both of latter reveled weaker intensity. These results suggest that the aggregation property of ADSCs during high-density culture would be dependent on the specific components of serum, and that JAM2 molecule could play a role in the animal sera-induced aggregation in vitro.

Melatonin plays pleiotropic roles in both animals and plants. Among them, the possible role of melatonin in the innate immune response in plants was emerging recently. As an initial study, we employed Arabidopsis to see whether melatonin is involved in the defense system against a virulent bacterial pathogen Psudomonas syringae DC3000. It was obviously observed that melatonin application of 10 μm concentration onto Arabidopsis and tobacco leaves induced various pathogenesis-related (PR) genes as well as a series of defense genes activated by salicylic acid (SA) and ethylene (ET), two key factors involved in the plant defense response compared to the mock-treated Arabidopsis and tobacco leaves, respectively. The induction of these defense-related genes in the melatonin treated Arabidopsis was well matched with an increase in resistance against pathogenic bacterium by suppressing its multiplication with about 10 fold relative over the mock-treated Arabidopsis. Furthermore, melatonin induced PR genes were almost completely or partially suppressed in npr1, ein2, and mpk6 Arabidopsis mutants indicative of SA and ET dependency of melatonin in plant defense signaling. These results suggest that melatonin may play a novel defense signaling molecule in plant-pathogen interaction