주로 리그닌을 분해하여 나무에 기생하는 백색부후균은 순수분리 및 균사체배양이 비교적 쉽게 이루어지며, 각종 난 분해성 물질을 비특이적으로 분해할 수 있는 특성 갖고 있다. 이러한 특성을 산업적으로 활용하고자 19종 22균주에 대하 여 Congo Red 등 5종의 염료에 따른 탈색율을 비교한 결과 를 보고하고자 한다. 기본배지 PDB는 pH 4.8~5.0, YPL배 지는 pH5.7~5.9로 염료종류별 차이는 없었고, PDB배지에 첨가한 염료종류별 탈색율은 Congo Red와 Bromophenol Blue에서 우수하였고, YPL배지의 경우는Congo Red, Methylene Blue, Bromophenol Blue에서 우수하여 YPL을 기본배지로 사용하는 것이 보다 광범위한 염료를 분해 할 수 있었다. 다양한 염료에서 탈색율이 우수한 유관버섯 등 3종 을 선발하였으며 앞으로 한계염료농도구명 및 산업적 활용 을 위한 계속적인 연구를 추진할 계획이다.
Spent coffee grounds, activated sludge, chicken manure, and agricultural waste-derived biochar were used to manufacture eco-friendly and functional compost via the bioaugmentation of white rot fungus and plant growth that promotes beneficial microorganisms. Six lab-scale composting reactors were established to perform composting. After composting was completed over 45 days, the composts were analyzed for major elements, physico-chemical characteristics, compost maturity, and compost effectiveness on crop growth and quality. Concentrations of T-N and PO4 3− significantly increased in the composts that had been amended with biochar and/or white rot fungus compared to the control, while those of NO3 −-N, TOC and TOC/T-N had significantly decreased, indicating the occurrence of effective composting. Besides, the germination indices of these composts were also generally higher than the control by 10-34%, indicating that the composts were mature. The four composts amended with biochar and/or white rot fungus (TR-3, TR- 4, TR-5, TR-6) also appeared to stimulate more growth in lettuce compared to commercial organic fertilizers (by 36- 104%). Besides, composts TR-3 and TR-4 respectively enhanced DPPH scavenging activity in lettuce leaves by 58% and 49%, while TR-4 and TR-5 respectively enhanced the total phenolic content (TPC) by 44% and 37%. This implies that the amendment of biochar and the bioaugmentation of white rot fungus could facilitate the composting process for the production of quality functional compost that is able to enhance the antioxidant content in crops. Quality composts could better compete with the commercially available fertilizers in the market, leading to the eco-friendly recycling of organic wastes such as spent coffee grounds, sludge, chicken manure, and agricultural waste.
Decolorization of congo red, rhodamine B was investigated by the white rot fungus Irpex lacteus which has biodegrading capability of various recalcitrants. White rot fungus Irpex lacteus is immobilized by PVA-freezing method. An immobilized Irpex lacteus decolorizes 91% of congo red in 8 days under culture with glucose 2%(initial conc.). It also showed 70% of decolorization at 3 days in the state of putting MnSO4 1mM.
But, rhodamine B has no significant differences about decolorization among different mixture ratio of Irpex lacteus with PVA, concentration of carbon, nitrogen and manganese sulfate.