The identity of toxin producers remains only hypothesis unless there were identified by strain isolation and analytical confirmation of both the cyanotoxin production and the genetic identity of the monoculture. The purposes of this study were to identify a morphologic and phylogenetic classification in Aphanizomenon flos-aquae strains isolated from the Nakdong River and to investigate the potential ability of the strains to produce toxins such as saxitoxin and cylindrospermopsin using target genes. The 16S rRNA and sxtA, sxtI, cyrA, cyrJ genes were analyzed on two strains (DGUC001, DGUC003) isolated from the Nakdong River. Morphological features of the strains were observed a shape of aggregated trichomes in parallel fascicles which can reach up to macroscopic size and a hyaline terminal cell without aerotope. In addition, the 16S rRNA phylogenetic analyses showed that the strains were identified as the same species with high genetic similarity of 98.4% and grouped within a monospecific andsupported cluster I of Aphanizomenon flosaquae selected from GenBank of the NCBI. The cyrA and cyrJ genes encoding for the cylindrospermopsinbiosynthesis were not detected in the present study. The sxtA gene was in detected both the two strains, whereas the sxtI gene which had been suggested as a suitable molecular marker to detect saxitoxin-producing cyanobacteria was not found both the strains. Thus, the two strains isolated from Nakdong River were identified as the same species of Aphanizomenon flos-aquae Ralfs ex Bornet et Flahault 1888, the two strains were confirmed as potential non-producing strains of the saxitoxin and cylindrospermopsin.

This study is the compared seismic performance that are difference between the performance of structures on various site classes and beam-column connection. this analysis model was designed the previous earthquake load. To compare the performance levels of the structure was subjected to nonlinear static and nonlinear dynamic analysis. Nonlinear analysis was used to The Perform 3D program. Nonlinear static analysis was compared with the performance point and Nonlinear dynamic analysis was compared the drift ratio(%). Analysis results, the soft site class of the displacement was more increase than rock site classes of the displacement. Also The smaller the displacement was increased beam-column connection stiffness.

본 연구에서는 비선형 정적해석법인 능력스펙트럼 법을 이용하여 성능목표를 만족하기 위하여 필요한 점탄성 감쇠기를 설계하는 절차를 제시하였다. 점탄성 감쇠기의 적정 크기를 구하기 위해 목표 변위에서 필요한 유효감쇠 비를 구한 다음 구조물의 이력거동에 의한 등가감쇠 비와 고유감쇠 비를 이용하여 필요한 감쇠기의 감쇠를 구하였다. 점탄성 감쇠기를 설치할 경우에는 구조물의 감쇠뿐만 아니라 강성도 변화하기 때문에 반복계산이 필요하게 된다. 본 연구에서는 먼저 단자유도계에서 구조물의 설계변수를 변화시키면서 제안된 방법의 타당성을 검증하였다. 또한 10층의 철골조 건물에 적용하고 지진응답을 구하였다. 제안된 방법에 따라 설계된 감쇠기를 설치하고 수행한 시간이력해석 결과에 의하면 고려된 설계변수에 관계없이 목표변위를 만족하는 것으로 나타났다.

The purpose of this study is to use crushed gravel for eco-friendly water-purification according to concentration in porous concrete.