A three-staged cascade virtual impactor was designed, fabricated, and used as aerosol classifier in a dust sensor module. The dust sensor module consisted of the impactor, three commercial dust sensors, and four pumps. For the design of the impactor, three cut-off diameters of 2.5 ㎛, 2.0 ㎛, and 1.5 ㎛ were selected. Then three nozzle widths were determined from computational fluid dynamics (CFD) simulation with the three designed cut-off diameters. </br>Laboratory generated PM2.5 aerosols classified into each of three sizes, via the fabricated impactor, entered a dust sensor from which voltage signals were detected due to particle scattering by a laser diode in the sensor. The voltage signal data from the three sensors were converted to number concentrations of the dust particles utilizing correlation equations obtained from separately performed experiments. The number concentrations were in agreement with those obtained by aerodynamic particle sizer (APS).

Fish larvae are immediately exposed to microbes from hatching to maturation of their lymphoid organs, therefore effective innate mechanisms is very important for survival in such an environment. The key component of innate immune system, C3 is central protein of all activation pathways of the complement system, leading to inflammatory reactions, such as opsonisation, chemotaxis, and cell lysis of pathogens. Although, innate mechanisms is essential for survival in the early stage of development, little is known about defence mechanisms. In this study, the alignment analysis showed that amino acid sequence of C3 from olive flounder liver EST homologous to other known C3 sequences with 73-99% identity. Also, we examined the tissue distribution of olive flounder C3 and analyzed expression pattern from the fertilized egg until 28 days post hatching. As a result, olive flounder C3 mRNA was expressed only in the liver and the mRNA level more increased as developmental proceed during the early stage. These results may suggest that olive flounder C3 plays an important function in the early immune response of olive flounder larvae.