붕어(무게 28.1±3.7 g, 길이 10.0±1.0 cm)에 0, 2.0×104, 2.0×105, 2.0×106, 2.0×107 CFU/ml의 Aeromonas hydrophila를 주사 후 2주간 실험을 실시하였다. A. hydrophila로 인위감염한 Carassius carassius의 2주 뒤 반수치사농도는 19.776×105 CFU/ml이었다. 혈액학적 지표에서는 A. hydrophila에 의해 혈색소와 적혈구 수가 유의하게 감소한 반면, 적혈구 용적은 유의적 변 화가 없었다. 마그네슘, 칼슘과 같은 혈장 무기 성분은 유의적으로 감소하였다. 유기 혈장 성분 에서 A. hydrophila 인위감염에 의해 혈장 포도당과 콜레스테롤이 유의하게 증가한 반면, 총 단 백질은 유의하게 감소하였다. 혈장 효소 성분인 ALP는 A. hydrophila 인위감염에 의해 유의하 게 증가하였다. 본 연구의 결과는 C. carassius에 대한 A. hydrophila의 인위감염이 치명적인 병원성 세균으로 혈액학적 성상 및 혈장 성분에 유의한 생리학적 변화를 유도하였음을 의미 한다.

Motile aeromonad septicaemia (MAS) mostly caused by Aeromonas hydrophila is a disease of high economic impact in freshwater organism. This study examined the seroconversion and seroprotection of formalinised local isolates of Aeromonas hydrophila in the Clarias gariepinus administered via different routes. Highly virulent Aeromonas hydrophila was treated with 40v/v formalin for 24 h. Bacterin was suspended to 0.5 McFarland standard and utilized to immunize Clarias gariepinus of 400±20 g in three groups with 50 samples each via three routes of administration. Antibody measurement taken at regular intervals before and after vaccination revealed a significantly higher antibody values in all routes compared to the control and difference was noticed at all intervals. Relative percent survival was 71.43, 62.86 and 57.14 intraperitoneal, intramuscular and immersion routes, respectively. Correlation analysis established a strong and direct relationship in the antibody generation through route of administration and efficacy of vaccine. This local vaccine was able to confer a high degree of immunity on Clarias gariepinus and can therefore be used to vaccinate against MAS.

Antimicrobial stability of grape seed extract (ActiVinTM), pine bark extract (Pycnogenol®), and oleoresin rosemary (Herbalox®) on the growth of Aeromonas hydrophila was investigated in cooked ground beef. When compared to the control, the populations of A. hydrophila were most effectively reduced by 4.06 log CFU/g for 1% Pycnogenol® added after cooking at 10 days of refrigerated storage, followed by 3.06 log CFU/g for 1% Pycnogenol® added before cooking and 1.36 log CFU/g for ActiVinTM. Bacteriostatic and bactericidal activities were observed for Pycnogenol® added before and after cooking, respectively. Pycnogenol® consists of heat-labile and heat-stable compounds. ActiVinTM and Pycnogenol® could be considered for use as multifunctional preservatives in meat and meat products.

폐수처리 중 생물학적 처리에 활용할 수 있는 질소분해 능력을 가진 미생물을 분리하여 동정하고자 경기도내 하천 6지점에서 채취한 시료로부터 50개 균주를 선별하였다. 형태학적, 생화학적 및 배양학적 실험결과 Bergey`s mannual of systematic bacteriology의 색인을 통하여 Aeromonas hydrophila로 동정하였다. Aeromonas hydrophila(AH-1). (AH-3), (AH-4), (AH-6) 균이 질산화 능력이 우수하였다. 4개 균주 모두 amoxillin, ampicillin, cephalothin과 ticarcillin에 내성을 나타내었다. 본 실험에서 분리한 .Aeromonas hydrophila의 질산화의 최적조건은 균 농도 1.0×l0exp(6) cells/ml, 배양온도 37℃로 나타났다.

This study was carried out to check changes of components in the slaughter waste by the bacteria isolated from slaughter wastes from Gyungnam Province from May to June 2000, and to find useful organism for treatment of the waste. Bacteria used in this study were Aeromonas hydrophila, as the dominant of the waste. Optimum conditions for bacterial culture were obtained as the temperature of 35℃, pH 6.5, and shaking of 120 rpm in nutrient broth. The mean values of dissolved oxygen was 4.14 ㎎/l; biochemical oxygen demand, 1731.21 ㎎/l; ratio of BOD/COD, 0.53-0.64; ratio of T-P/T-N, 1.0-1.41; and viable counts of the waste, 5.47×107 CFU. Little change in total nitrogen observed by 36 hr of the culture. The largest amount of increasing NH4+-N was observed in the sample that 10% of the waste added in nutrient broth with A. hydrophila showing the value of 29.19 ㎎/l at the beginning to 570.36 ㎎/l by 36 hr of culture. However, the highest increasing ratio between initial amount and finals at 36 hr of culture showed as 41.6 times when 3% of the waste added. NO3--N was decreased showing the value of 71.27 ㎎/l to 32.14 ㎎/l by 24 hr of culture with the organism when 10% of the waste added in nutrient broth. Total phosphorus was decreased showing the value from 188.74 ㎎/l to 101.41 ㎎/l after 12 hr of culture with the organism when 5% of the waste added in nutrient broth, while T-P was decreased gradually by 24 hr of culture from 193.8 to 101.4 ㎎/l when 10% of the waste added.