The population of managed honeybees has been dramatically declining the recent past in worldwide. N. ceranae causessignificant detriment to honey production and results in economic losses critically. In our knowledge, Fumagillin is theonly antibiotic approved for control of nosemosis in honeybees. In this study, to select isolate with anti-Nosema activityagainst N. ceranae. Entomopathogenic fungi cultural filtrates were screened using in vitro polar tube germination assay.These fungi cultural filtrates were used to evaluate the safety of honeybees and their inhibition of nosemosis. As a result,P. marquandii 364 and Pochonia sp. 60 showed inhibitory activity on the growth of N. ceranae in honeybees and didnot significantly affect the survival rate of honeybees. These may be employed as antibiotic agents and a good featureto be used in the development of new biocontrol agents of nosemosis.

The population of managed honey bees has been dramatically declining the recent past in worldwide. The one of most common disease of bees is nosemosis, the nosemosis is caused by microsporidia in the genus Nosema. Nosema apis and N. ceranae have been described as honeybee pathogens. These microsporidia are highly evoloved fungi with an obligately intracellular parasitic lifstyle. The disease causes significant detriment to honey production and results in economic losses. In our knowledge, Fumagillin is the only antibiotic approved for control of nosemosis in honey bees, however this antibiotic may have unintended effects on the honey bee host, ultimately contributing to increased prevalence and pathogenicity of Nosema. Therefore, we screened anti-Nosema substances from entomopathogenic fungal culture filtrates using in vitro polar tube germination assay. These fungal metabolites are employed as antibiotic agents. As results, Total 3 samples (23% of 13 total samples) showing the germinating inhibition against N. ceranae. This screening method may be useful for the detection of anti-Nosema substances from various samples and selected samples in this study may be a good feature to be used in the development of a new biocontrol method of nosemosis.

Since airborne fungi have been known to aggravate indoor air quality, studies on developing anti-fungal filters increase recently. In this study, silver (Ag) nanoparticle was selected as anti-fungal agent. HEPA filter was coated with silver nanoparticles which were generated via spark discharge system operating at atmospheric pressure and temperature. The anti-fungal effect of the Ag-filter was evaluated with the conventional culture assay. When the number of Ag nano particle per a fungal particle in the filter was 1.91X106, the fungicidal efficiency was higher than 99%. As another anti-fungal test, ATP bioluminiscence detection method was also carried out and the results were correlated with those of the culture assay.

Many higher fungi were collected at Korea from 1976 to 1998. They were identified and surveyed on resources with many reference books. According to the results, fungal fungi were 40 families, 90 genera and 215 species. Among them, anti-cancer resources used in Korea were Ganoderma lucidum, Phellinus linteus, Agaricus brazei and Cordyceps militaris. Three species exception Agaricus brazei were distributed in Korea. All these are cultivated in Korea.

Many higher fungi were collected at Korea from 1976 to 1998. They were identified and surveyed on resources with many reference books. According to the results, fungal fungi were 40 families, 90 general and 215 species. Among them , anti-cancer resources used in Korea were Ganoderma lucidum, Phellinus linteus, Agaricus brazei and Cordyceps militaris. Three species exception Agaricus brazei were distributed in Korea. All these are cultivated in Korea.