Soybean aphid, Aphis glycines Matsumura has been identified as a pest of soybean in Korea, Japan, and China. The use of synthetic chemical insecticides is a major option to manage this pest, but the demand for environmental-friendly insecticide is increasing. Entomopathogenic fungus is one of the alternatives which is safe for the environment. In this bioassay, leaf dipping method was used to evaluate Beauveria bassiana (Balsamo) Vuillemin ARP14 and GHA. The mortality of both adults and nymphs was not significantly different between the two fungus strains. However, faster mortality rate in both life stages of aphid was found in ARP14 compared to GHA strain. Also, the mycosis rates of both adults and nymphs were higher in ARP14 than in GHA strain. Our results suggest that B. bassiana ARP14 can be an effective and an environmental-friendly control agent for A. glycines.

Ten novel microsatellite loci were isolated and characterized from the soybean aphid, Aphis glycines. The soybean aphid was recently introduced into North America where it has become a serious pest of soybeans. This invasive pest has rapidly spread throughout the midwestern United States and southern Canada since 2000. The isolated loci were polymorphic, with two to 18 alleles in 20 individuals from a single population in Korea. The analyses revealed that 19 individuals had different multilocus genotypes, showing expected heterozygosity values ranging from 0.180 to 0.891. We report the development of microsatellite markers for A. glycines potentially suitable for further studies of population structure, dispersal, and host alternation.

1987년 5월부터 9월 사이에 대전근교의 콩에 기생하고 있는 콩진딧물에서 진디벌에 기생당한 mu-mmy를 채집하여 진디벌과 중기생벌에 관한 생물학적 연구를 수행하였다. 진딧물의 진디벌에 대한 기생율, 진디벌과 중기생벌의 기주선택, mummy형성수와 형성 비율, 주요 진디벌과 중기생벌 종의 수명 등을 조사한 결과는 아래와 같다. 177개의 채집된 진딧물 mummy 중 진디벌은 27.1%m 중기생벌은 50.3%였다. Aphidius cingulatus, Ephedrus persicae, E. plagiator가 콩진딧물에 기생하고 있는 진디벌의 주요 종으로 나타났다. 그리고 이들 진디벌에 기생하고 있는 중기생벌들 중에 A. vulgaris, A. convexa가 우점으로 나타났다. 이들 중기생벌중 Charios brasicae는 한국 미기록종이었다. E.plagiator가 다른 콩진딧물에 기생하는 진디벌에 비해 중기생벌에 적게 기생되었다. 기생능력은 A. cingulatus 보다 E. plagiator가 더 높은 것으로 나타났다. 진디벌과 중기생벌의 수명은 중기생벌이 3일~29일, 진디벌은 1일~4일로 추정되었다.

포장내에서 실수조사에 의한 진딧물 발생상황과 살충제(Carbofuran 립제, Disulfoton 립제, Ortra 분제)의 약량을 달리한 파구처리효과를 검토해 본 결과 아래와 같다. 1. 콩에서는 싸리수염진딧물과 콩진딧물이 우점종으로 전자는 작물생육 초기와 후기에, 후자는 7월 중순(중기)에 밀도가 높았으며 황색수반에 유살된 싸리수염진딧물은 초기 및 후기 유살수가 높았으나 콩진딧물은 후기에 낮았다. 2. 입묘율은 Carbofuran구에서 높았고 Disulfoton과 Ortran구는 낮았으며 약해는 Carbofuran구에서 심하였으나 기타는 경미하였다. 3. 바이러스 이병율은 로 낮았으며 약제 및 약량간에 차이가 없었다. 4. Carbofuran, Disulfoton은 처리후 65일까지 진딧물 방제효과가 인정된 반면 Ortran은 51일부터 효과가 떨어지는 경향이었다. 5. 수량은 약제 및 약량간에 차이는 있으나 일정한 경향이 없는 것은 생육말기의 도복이 큰 원인으로 생각된다.

Native of soybean aphid (Aphis glycines Matsumura) is an Asia and aphid is one of the dangerous pests in soybean [Glycine max (L.) Merr.]. High density aphid populations can reduce crop production by causing severe damage. The objective of this study was evaluation of resistance to the soybean aphid in soybean cultivars and germplasms. A total of fifty five soybean cultivars or germplasms, including two susceptible and two resistant check varieties, were infested to evaluate their resistance in the field cage and greenhouse test by aphid colonies which derived from wild collected one soybean aphid biotype in Korea. The average number of reproduced soybean aphid was evaluated with 62.7 aphids in the resistant check variety PI 567598B and also estimated with 1,807 aphids for susceptible check variety Williams 82. In soybean varieties and germplasms, the average reproduced soybean aphid populations ranged from the lowest 497 aphids for Junjeori to the highest 3,862 aphids for Mansu. About seventy six percent of soybean cultivars and germplasms were shown high density soybean aphid populations when compared with another susceptible check variety PI 567543C in the field cage test. From the greenhouse test to evaluate aphid index, 87.3% of soybean cultivars or germplasms presented aphid index with 9.0. No soybean cultivars and germplasms were observed with soybean resistant phenotype when regarded a aphid resistant level as less than 10% aphid reproductions compared with susceptible check Williams 82. Although no Korean soybean cultivars were identified with resistant trait to the soybean aphid, we found one great resistant genetic resource PI 567598B in this study. This result will be helpful to further study for providing useful genetic information for soybean researchers.