본 연구는 국내에서 육종한 품종인 ‘Pink Aurora’ 장미를 보존화로 가공하는 기술의 개발에 관한 것으로 탈수와 탈색 처리를 위한 에탄올의 침지시간과 염색을 위한 보존용액의 온도와 침지시간를 구명하고자 수행하였다. 탈수는 먼셀 H값에서 붉은 색이 완전히 빠지고 시간이 길어질수록 황색이 짙어짐을 고려해 볼 때 12시간이 가장 효과적이었다. 보존용액 처리에 서는 채도값을 나타내는 C의 차이는 크지 않았지만 온도가 높고 처리시간이 길어질수록 색의 맑기가 높아졌다. 낮은 온도 에서 침지시간이 길어짐에 따라 화형의 감소율이 낮았다. 24 시간 건조 후 무게는 모든 온도 처리에서 침지시간이 길어질 수록 53~47% 감소하였고, 건조 48시간의 25℃에서 6시간 침 지에서 73%, 24시간 침지에서 68%의 감소율을 나타내 침지시간이 길어질수록 감소율이 낮았다. 따라서 에탄올로 12시간동 안 탈수하여 염색 보존액을 25℃에서 24시간동안 처리하는 것이 가장 효과적이라고 생각된다.

Aurora A kinase is a mitotic serine/threonine kinase whose proposed functions include the maturation of centrosomes, G2/M transition, alignment of chromosomes at metaphase, and cytokinesis. In this study, we investigated the effect of MLN8237, an aurora A kinase inhibitor, on the postovulatory aging of oocytes based on the frequency of oocyte fragmentation, cdk1 kinase activity, and cyclin B degradation. The fragmentation of ovulated oocytes during prolonged culture was inhibited by treatment with MLN8237 in a concentration-dependent manner. The frequency of fragmented oocytes was significantly lower in oocytes treated with 2 μM MLN8237 (13%) than in control oocytes (64%) after two days of culture. Most of the control (non-fragmented) oocytes (91%) were activated after two days of culture. In comparison, only 22% of the MLN8237-treated oocytes were activated; the rest of the oocytes (78%) were still in metaphase with an abnormal spindle and dispersed chromosomes. Next, cdk1 activity and the level of cyclin B were examined. The level of cyclin B and cdk1 activity in MLN8237-treated oocytes were nearly equal to those in control oocytes. Our results indicate that MLN8237 inhibited the fragmentation of ovulated oocytes during prolonged culture, although it blocked the spontaneous decrease in activity of cdk1 and degradation of cyclin B. This mechanism of inhibition is different from that in oocytes treated with nocodazole, which have high levels of cdk1 activity and cyclin B.