본 연구는 혐기소화액을 클로렐라 배양 배지로 이용하여 클로렐라 처리 농도 및 침종 처리방법에 따른 고추 종자의 발아율에 미치는 영향을 구명하고자 수행하였다. 고추 종자는 증류수(대조구), 클로렐라 배양액의 50% 희석액(50%), 클로렐라 배양원액(100%), 클로렐라 150, 200, 400% 농축액, 클로렐라 배양여액, 대조구를 두었다. 클로렐라 배양액의 무침종 처리구가 86.7~95.6%의 발아율을 나타낸 것과 비교해 볼 때, 침종 처리구의 발아율은 98.3~100%로 5~12% 높은 발아율을 나타내었다. 그러나 클로렐라 배양여액 처리구의 발아율은 73.3%로 클로렐라 배양액 보다 낮았다. 클로렐라 침종처리구의 발아속도는 무침종 처리구보다 1일 빨랐다. 고추 종자의 80% 발아 소요 시간은 클로렐라 배양액 처리구가 대조구보다 1.1~1.2일 빨랐다. 그러나 클로렐라 배양여액 처리구의 발아속도는 클로렐라 배양액 보다 낮았다. 클로렐라 배양액 150% 침종 처리구의 유근길이는 6.23cm로 대조구보다 약 2cm 더 길었다. 상대적 유근신장률은 클로렐라 150% 침종처리구에서 146%로 대조구보다 46% 높게 나타났다. 클로렐라 배양액 처리구의 고추 종자 발아지수(GI)는 130~150%로 무처리구 보다 높았다. 클로렐라 배양액 처리는 고추 종자 발아율이 높았고 발아기간이 단축되고 유근이 길어지는 효과를 나타내었다. 결론적으로 클로렐라 배양액은 고추 종자 발아에 안정성이 높았고 종자의 발아 촉진에 유용하게 적용이 가능하였다.

This experiments were conducted to evaluate the influence of Chlorella culture solution using anaerobic digestate as medium on seed germination of perennial ryegrass seeds. Four treatments were compared: control with distilled water, anaerobic digestate, Chlorella culture solution and Chlorella culture filtrate. The germination percentage of perennial ryegrass seeds was highest in the Chlorella culture solution treatment. Days required for 50, 70% seed germination were faster at 1.7 day in Chlorella culture solution compared to control. Root length of perennial ryegrass seeds was longer by 1~2cm in the Chlorella culture solution compared with control. The relative root length was by 40% longer in the Chlorella culture solution treatment compared to control. The germination index (GI) of perennial ryegrass seeds was higher by 180~202% in the Chlorella culture solution treatment compared to control. The decay rate was low as 50.0% in Chlorella culture solution, but decay rate of perennial ryegrass seeds showed 86.7~83.3% in control plot and in anaerobic digestate, respectively. Chlorella culture solution have shown stimulatory effects in germination and development of root. Overall, Chlorella culture solution could be useful to apply for promotion of germination and root elongation of seeds.

Anaerobic digestion is a collection of naturally occurring processes that convert organic matter and liquid residue, so-called digestate. The use of digestate biofertilizers is one of the important components of integrated nutrient management, as they are renewable sources of plant nutrients for sustainable agriculture. Seeds of Italian ryegrass (Lolium multiflorum L.) were germinated in different concentration of Chlorella in order to investigate it’s the effect of Chlorella on growth parameters, seed germination and early growth. The experiment using plug tray was conducted at the green house placed in the Sangji University. The experiment consisted of nine treatments including different concentrations of Chlorella sp. culture solution and non-treated control. The germination percentage at the treatment with 25% Chlorella sp. culture solution was greater than that of control. The 50% concentration of Chlorella sp. culture solution was found to promote a better seedling growth in terms of shoot length, fresh weight and dry weight compared to the anaerobic digestate. Results showed that the best concentration of Chlorella culture solution was achieved by the 50% concentration of Chlorella culture solution treatment. As a conclusion, the application of Chlorella culture solution was found to be able to promote the germination and shoots growth of Italian ryegrass