Various eco-friendly seed disinfection technologies have been developed due to the increase in the global demand for organic food. In order to produce sustainable and eco-friendly agricultural products in Korea, seed disinfection technologies for the production of wholesome seedlings have been researched in diverse ways compared to how they were examined in advanced countries. Both domestically and internationally, the eco-friendly seed disinfection technologies to secure the horticultural crops have been treated by biological or physical methods, such as hot water treatment with ultrasonic disinfection technology, or applying organic agricultural materials like organic acids in plant extracts. However, from a practical perspective, various applied technologies can be implemented in farm fields to disinfect significant agricultural crops, such as lettuce, Chinese cabbage, radish, watermelon, cucumber, paprika, red pepper, ginger, ginseng, and sesame seeds.