This study was conducted for SNPs in the 5'-regions of estrogen receptor-α(ESR-α), and association with calving interval (CI), service per conception (SPC) and 305 days milk yield in Hanwoo and Holstein dairy cattle. The genet-ic improvement was incurred low reproduction performance. The objective of this study was to investigate connec-tions between single nucleotide polymorphisms (SNP) of Estrogen receptor-α (ESR-α) with reproduction performance (calving interval, service per conception, and 305 d milk yield) in Hanwoo and Holstein dairy cattle. Hanwoo and Holstein blood samples were collected from 183 and 124 dam of breeding farms and DNA was extracted. Primer design was based on NCBI GenBank (Accession No. AY340579). The PCR-RFLP method with Bgl I was used to ge-notype the cattle. The result showed two variants of the ESR-α gene. The Bgl I cut the 492 bp amplification pro- duct into 322 bp and 170 bp fragments for allele G, while allele A remained uncut, resulting in two restriction frag-ments for homozygote G/G and three fragments for heterozygote A/G. We found two of different genotypes in the-se breeds, A/G and G/G. In Hanwoo, the A/G genotype frequency was 0.13, and G/G was 0.87. The CI of A/G was 382.18±10.03 days, and G/G was 381.69±5.22 days. The SPC of A/G was 1.62±0.16, and G/G was 1.32±0.04. While CI showed no significance difference, SPC exhibited significant difference (p<0.05). In Holstein cattle, the frequency of genotype A/G was 0.02 and G/G was 0.98. The 305 days milk yield of A/G was 7,253.00±936.00 kg and of G/G was 8,747.51±204.88 kg, showing no significant difference.

구조적으로 estrogen 수용체(estrogen receptor, ER)와 유사한 estrogen receptor-related receptor(ERR)는 포유동물에서 배발생 후기에 외배엽 형성과 관련되어 있다고 알려진 고아핵수용체(orphan nuclear receptor)이다. ERR은 ER과 DNA binding domain의 보존성은 유사하지만, ligand 결합 및 전사 활성은 다르다. 포유동물의 ERR에 관한 연구에 비하여 해양 무척추동물의

Transforming growth 신호의 매개자 역할을 하는 Smad 계열 단백질은 발생과정에 중요한 역할을 한다고 알려져 있다. Estrogen receptor(ER)와 구조적으로 유사한 estrogen receptor-related receptor(ERR)은 포유동물에서 후기 배발생기에 외배엽 형성과 관련이 되어 있는 고아핵수용체이다. 본 연구에서는 해양무척추동물의 초기발생과정과 계절번식기 동안에 Smad3와 ERR의 유전자 발현이 발생과정과 성숙

대부분의 내분비교란물질들은 에스트로겐이나 항에스트로겐적인 활성을 가지고 있어 사람이나 생태계에 있어서 생식기능 발달을 저해하는 것으로 보고되고 있다. 본 연구는 estrogen (E)과 bisphenol (BP) 그리고 octylphenol (OP)이 생쥐 Leydig cell line인 TM3 세포에 미치는 영향을 알아보고자 하였다. TM3 세포는 11 ~13일령의 BALB/c nu/＋ 생쥐로부터 유래한 정소내 세포인 Leydig cell로 DMEM에