은행잎에서 유래된 ginkgetin과 isoginkgetin을 피부 미용 측면에서 생리활성을 연구하여 화장품 소재로서 가능성 여부를 규명하고자 하였다. Ginkgetin과 isoginkgetin을 분리하여 세포 독성 실험을 통해 5 μM 이하 농도에서 실험을 실시하였다. NO 저해능 실험에서 ginkgetin이 isoginkgetin보다 더 강력한 저해활성을 나타냈고 iNOS, IL-1b, IL-6 등 염증 인자의 mRNA 발현은 감소되었으나, 항산화 효소의 발현 변화는 거의 없었다. MMP-1 발현 저해활성은 isoginkgetin이 더 우수했고 그 기작은 JNK 활성 감소에 의한 것임을 확인하였다. 한편, 멜라닌 생성 저해능은 알부틴 보다 우수하였으며, 그 기작은 MITF와 Tyrosinase 발현을 억제하는 것에 의한 것임을 확인하였다. 이상의 결과를 종합하면, 은행잎 추출물 유래 ginkgetin과 isoginkgetin은 세포독성이 다소 나타났으나, 주름개선과 미백 화장품의 소재로 매우 유용하게 활용될 수 있을 것으로 사료된다.

Hepatic ischemia-reperfusion injury (HIRI) is linked with high mortality rate. Several agents have been developed so far to reduce the risk of HIRI. In this study, we investigated the effects of combined treatment of Ginko biloba leaves extract and vitamin C (GLEVC) on hepatic ischemia-reperfusion injury. To explore the protective effects of GLEVC on HIRI rats model were tested. After the development of HIRI by using clamping method rats were then randomly divided into four groups. Different doses of GLEVC were administered in HIRI rat model. The level of ALT, AST, SOD and MDA content in serum were detected in HIRI groups. Moreover, the activity of SOD, content of MDA, and GSH in hepatic tissue were also examined. Bcl-2 and Bax protein expression were detected by immunohistochemical staining method. Compared with sham group, GLEVC has the protective effect on the HIRI-induced model. Level of ALT, AST, and MDA in blood were significantly lower in GLEVC group compared with HIRI-induced group. Moreover, SOD activity and GSH were increased in GLEVC group whereas MDA content was reduced by GLEVC treatment. Furthermore, HIRI-induced Bax protein was reduced upon GLEVC treatment, whereas Bcl-2 protein expression was enhanced. These results demonstrate that GLEVC treatment may provide potential ameliorative therapy by reducing damaged signaling mechanism in hepatic ischemia/reperfusion injury model.

Objective The aim of this work was to ascertain whether the memory disturbance following scopolamine administration and the neuroprotective effect of could be evidenced in global cerebral ischemia by evaluating improved cognitive capacity in the rats. Materials and Methods Neuronal cell density was measured by counting viable cells in the left and right CA1 regions of three coronal sections of 30 um. Behavior test; Acquisition deficits after ischemia.. Use passive avoidance test. Results Neuroprotective effect of GB at 100mg/kg is 87.3%. Representative photomicrographs of cresyl violet-stained hippocampal regions of either sham-operated animals(A,B) or animals that had been subjected to 10 min ischemia followed by the treatment with either saline (C,D) or 100mg/kg of Ginkgo biloba (E,F). Boxed regions in A, C, and E are shown in B, D, and F, respectively. The 10 min ischemia caused selective and delayed neuronal cell loss in the hippocampal CA1 region (C,D). In contrast, GB treatment conferred neuroprotection by markedly reducing the number of damaged pyramidal cells in the CA1 subfield (E,F). Scale bar is 100 um. Effect of GB on scopolamine induced memory deficits in the passive avoidance test. At 30 min after trainining trials, scopolamine(1mg/kg i.p.) or the same volume of saline was administered to rats. At 30 min after scopolamine injection, the rats were treated with GB(100mg/kg). Acquisition trials were carried out 30 min after GB treatment. At 24 hr after acquisition trials, the test trials were carried out. Data represents mean ± SEM (n=6).