연구는 홍삼박 물추출물(RGW), 에탄올추출물(RGE), 1,3-B.G추출물(RGB)을 HPLC로 성 분 분석과 B16F10에 대한 세포생존율, 항산화능 및 주름개선 효능 평가를 실시하여 홍삼박 추출물의 화장품 소재로서의 응용가능성을 알아보았다.추출물의 HPLC 성분 분석 결과, 세 가지 추출물 모두 다양한 종류의 진세노사이드가 검출되었으며 그 중 RGB가 가장 많은 함량의 진세노사이드가 검출되었다. B16F10의 세포생존율 측정 결과, RGW와 RGB가 유사하며, RGE보다 높은 생존율을 보여주었다. DPPH radical 소거능 측정 결과 RGE>RGB>RGW 순으로 나타났으며 SOD유사활성능 측정 결과는 RGB>RGE>RGW순으로 활성능을 보여주었다. Elastase 저해능 측정 결과에서는 RGW>RGB>RGE 순으로 활성을 보였다. 위와 같은 결과 를 종합하여 볼 때 피부안전성이 우수하며 항산화 및 주름개선 화장품소재로서 RGW와 RGB가 적합할 것으로 판단된다.
Background: Some phenolics detected in the soil may inhibit the seed germination and seedling growth of ginseng (Panax ginseng). This study investigated the effect of irrigation and ginseng root residue addition on the soil microbial community and root rot disease in 2-year-old ginseng.
Methods and Results: Each 20 ℓ pot was filled with soil infected with ginseng root rot pathogens, and irrigated daily with 2 ℓ of water for one month. After the irrigation treatment, ginseng fine root powder was mixed with the irrigated soil at a rate of 20 g per pot. In descending order, NO3 −, electric conductivity (EC), exchangeable Na (Ex. Na) and K (Ex. K) decreased due to irrigation. In descending order, NO3 −, EC, Ex. K, and available P2O5 increased with the additon of ginseng powder to the soil. The abundance of Trichoderma crassum decreased with irrigation, but increased again with the incorporation of ginseng powder. The abundance of Haematonectria haematococca increased with irrigation, but decreased with the incorporation of ginseng powder. The abundance of Cylindrocarpon spp. and Fusarium spp., which cause ginseng root rot, increased with the incorporation of ginseng powder. The abundance of Arthrobacter oryzae and Streptomyces lavendulae increased with irrigation. The abundance of Streptomyces lavendulae decreased, and that of Arthrobacter spp. increased, with the incorporation of ginseng powder. Aerial growth of ginseng was promoted by irrigation, and ginseng root rot increased with the incorporation of ginseng powder.
Conclusions: Ginseng root residues in the soil affected soil nutrients and microorganisms, and promoted ginseng root rot, but did not affect the aerial growth of ginseng.
Background : Root rot is a major factors of replanting failure in ginseng cultivation. Some of the phenolics detected in the soil could inhibit the seed germination and seedling growth of ginseng.
Methods and Results : Water of 2 ℓ was irrigated per pot (20 ℓ) into the soil infected with ginseng root rot pathogens for one month every day. After the irrigation treatment, the powder of ginseng fine root of 20 g per pot was mixed with the irrigated soil. NO3 -, electric conductivity (EC), exchangeable Na (Ex. Na) and K (Ex. K) were decreased in descending order by irrigation. NO3 -, EC, Ex. K, and available P2O5 were increased in descending order by incorporation of ginseng powder into soil. Trichoderma crassum was decreased by irrigation, but it was increased again by incorporation of powder. Haematonectria haematococca was increased by irrigation, but it was decreased by incorporation of powder. Cylindrocarpon spp. and Fusarium spp. causing ginseng root rot were increased by incorporation of powder. Arthrobacter oryzae and Streptomyces lavendulae were increased by irrigation. Streptomyces lavendulae was decreased, and Arthrobacter spp. was increased by incorporation of powder. Aerial growth of ginseng was promoted by irrigation, and ginseng root rot was increased by incorporation of powder.
Conclusion : The residues of ginseng root in the soil affected soil nutrients and microorganisms, and promoted ginseng root rot, but did not affect the aerial growth of ginseng.