Known for its effectiveness in weight loss and diabetes prevention, Gymnema sylvestre products can be found in the US, Japanese, and Indian markets. However, the recommended dosage or safety of these products has not yet been proven. Therefore, development of an analytical method for detecting the content of Gymnema sylvestre in food products is required. Accordingly, this study proposes an analysis method that can examine Gymnema sylvestre in food using LC-ESI-MS/MS and KASP (Kompetitive Allele-Specific PCR) markers. In LC-ESI-MS/MS, a simultaneous analysis method for gymnemic acid and deacylgymnemic acid was optimized using negative ionization mode, and its validation test was completed for solid and liquid samples. In addition, KASP markers were prepared by finding the specific SNP of G. sylvestre in ITS2 and matK through DNA barcodes. The two KASP markers returned positive FAM fluorescence result when combined with G. sylvestre, and this aspect was confirmed in raw G. sylvestre as well. The applicability of the method was tested on 21 different food and healthy functional products containing G. sylvestre purchased on the internet. As a result, although there was a difference in the ratios of gymnemic acid and deacylgymnemic acid in LC-ESI-MS/MS, the index component was detected in all 21 products samples. In the KASP analysis, 9 products returned positive FAM result, and the rest of the products were found to be containing G. sylvestre extract. This study is the first study to use the dual system of LC-ESI-MS/MS and KASP for the analysis of G. sylvestre. The study has confirmed that these two methods are applicable to the examine G. sylvestre content in food products.

한국에서 ‘가가이모과잎'이라고 불리는 인도의 전통 식물 약재인 Gymnema sylvestre는 당뇨의 원인인 생체 내당의 대사를 조절하는 약재로 이용되어 왔다. 본 연구에서는 Gymnema sylvestre 잎으로부터 인공적으로 조직 배양한 캘러스를 대량 배양하였다. 배양된 캘러스부터 추출물을 얻은 후 지방 전구 세포인 3T3-Ll 세포를 이용하여 항비만 효과를 관찰하였다. 결과적으로, Gymnema sylvestre 잎으로부터 조직 배양한 캘러스 추출물은 지방 전구 세포의 지방세포로의 분화뿐만 아니라 세포 내 지질 축적을 억제 하였다. 또한 지방세포의 형성을 조절하는 초기 지방세포 내의 전사인자인 C/EBP-α 발현을 억제하면서 인슐린에 의해 유도된 지방세포의 분화를 억제하였다. 따라서 이러한 결과들은 피부를 위한 화장품 성분으로서 응용 가능성을 제공할 수 있을 것으로 사료된다.