In this paper, the instability of the domed spatial truss structure using wood and the characteristics of the buckling critical load were studied. Hexagonal space truss was adopted as the model to be analyzed, and two boundary conditions were considered. In the first case, the deformation of the inclined member is only considered, and in the second case, the deformation of the horizontal member is also considered. The materials of the model adopted in this paper are steel and timbers, and the considered timbers are spruce, pine, and larch. Here, the inelastic properties of the material are not considered. The instability of the target structure was observed through non-linear incremental analysis, and the buckling critical load was calculated through the singularities and eigenvalues of the tangential stiffness matrix at each incremental step. From the analysis results, in the example of the boundary condition considering only the inclined member, the critical buckling load was lower when using timber than when using steel, and the critical buckling load was determined according to the modulus of elasticity of timber. In the case of boundary conditions considering the effect of the horizontal member, using a mixture of steel and timber case had a lower buckling critical load than the steel case. But, the result showed that it was more effective in structural stability than only timber was used.