Mechanical stimulation has been reported to improve cell growth in bone and cartilage regeneration. Various research groups have described their own bioreactors that stimulate cell-seeded scaffolds. We have developed a bioreactor capable of applying controlled compressive loading to a cell-encapsulated agarose hydrogel. To compare the effect of mechanical stimulation on agarose hydrogel, we used one control group (static mode) and five experimental groups. The results show that the intermittent_3sec conditions improve proliferation of cells better than the other conditions studied.