This paper is concerned with the optimal design of hyperbaric oxygen therapy system chamber with separated rooms for multi-users using stress analysis. Hyperbaric oxygen therapy is very important medical remedy for increasing the concentration of melted oxygen in human and animal body to recover the impaired cell for the healthy condition. This research proposed the proper procedures to design the physical specifications and investigate the mechanical design safety of hyperbaric oxygen chamber with two separated rooms using computational stress analysis with Solidworks program. The result of this research will be used to make the real size model of practical hyperbaric oxygen therapy system for multi-users with separated chamber.

This paper is concerned with the mechanical design of hyperbaric oxygen chamber for multi-users using stress analysis. HBOT(Hyperbaric Oxygen Therapy) is very effective medical equipment for increasing the concentration of melted oxygen in human and animal body. The hyperbaric oxygen supplies to the impaired cell of human and animal to recover the healthy condition. This research reported the design specifications and mechanical safety of hyperbaric oxygen chamber using computational stress analysis with CATIA program. The result from this research can be used for making the practical HBOT equipment for multi-users and to manufacture the real model

This paper is concerned with formulating the design specification and making hyperbaric oxygen chamber for multi-users. HBOT(Hyperbaric Oxygen Therapy) is very effective remedy for increasing the concentration of melted oxygen in human and animal body. The melted oxygen supplies to each cell in body to recover the normal condition. This research reported the design specifications and mechanical test results of hyperbaric oxygen chamber made with polyurethane as well as computational analysis with CATIA program. These data can set the physical dimensions of practical HBOT equipment for multi-users and be used to produce the effective commercial model.

Human gingival fibroblasts (hGFs) were reported to play an important role in inflammatory reactions to lipopolysaccharide (LPS) from P.gingivalis in the periodontal connective tissue. Although the biostimulatory effects of hyperbaric oxygen therapy, such as anti-inflammatory activity, have been reported, the pathological mechanism is not completely understood. This study examined the changes in the inflammatory cytokine profiles, which are produced after exposure to hyperbaric oxygen in P.gingivalis LPS-treated human gingival fibroblasts, and subsequently to examine the mitogen activated protein kinase (MAPK) pathway involved in cytokine production. Gingival fibroblasts with or without P.gingivalis LPS were exposed to hyperbaric oxygen, and the cytokine profiles in the supernatant were observed using a human inflammation antibody array. The expression of cyclooxyginase-2 (COX-2) protein, phosphorylation of extracellular signal-regulated kinase (ERK1/2), p38, and c-Jun-N-terminal kinase (JNK) MAPK by western blot analysis, and the amount of prostaglandin E2 (PGE2) in the supernatant by an enzyme-linked immunoassay were determined. COX-2 protein expression and PGE2productionwereincreasedsignificantlyintheP. gingivalis LPS-treated group, and were decreased by treating P. gingivalis LPS with hyperbaric oxygen. Treatment of P. gingivalis LPS in the gingival fibroblasts led an increase in the amount of pro-inflammatory-related cytokines interleukin-6 (IL-6) and IL-8 released, whereas hyperbaric oxygen inhibits the irrelease. Ananalysis of the MAPK signal transduction showed that hyperbaric oxygen induced a significant decrease in the level of P38 phosphorylation regardless of the presence or absence of LPS. In addition, hyperbaric oxygen promoted JNK phosphorylation, significantly in the presence of LPS. Hyperbaric oxygen can inhibit pro-inflammatory cytokines and mediate the MAPK signal pathway, and appears to be useful as an anti-inflammatory tool.