This study investigated various levels of sodium nitrite and probiotics (SNPro) combination as an alternative to zinc oxide on the growth performance, immune response, intestinal microflora, and morphology of weaned pigs. One hundred and ninety-two weaned pigs (Landrace×Yorkshire×Duroc) with an average body weight of 6.51±0.15 kg were randomly assigned to four treatments(n=6) on the basis of their initial body weight. Experimental period was divided into phase 1 and 2 (each 14 days). The dietary treatments were: 1) Basal diet (control), 2) SNPro1 (control+0.01% SNPro), 3) SNPro2 (control+0.02% SNPro), 4) SNPro3 (control+0.03% SNPro). The average daily gain when SNPro was added to the diet was 288, 309, 319, 324 g in phase 1, 355, 387, 410, 407 g in phase 2 and 321, 348, 364, 366 g in the overall. The concentration of interleukin-8 and interleukin-10 in serum when SNPro was added to the diet were 15, 13.5, 13, 12.8 ng/ml and 165, 162, 155, 145 ng/ml (p<0.05) but toll-like receptor 4 and immunoglobulin G levels in serum were no significantly different. The colonization of Escherichia coli in the ileum and Salmonella spp. in the caecum were significantly decreased as SNPro level increased (p<0.05). However, the population of Lactobacillus spp. did not differ among the groups. Although villus height and villus height to crypt depth ratio were not significantly affected by the treatments, crypt depth in the jejunum was 599, 586, 615, 599 ㎛ as SNPro level increased (p<0.05). In conclusion, SNPro had beneficial effects on growth performance, immune response, intestinal microflora and morphology weaned pigs. Therefore, SNPro not only can be considered as an alternative for the pharmacological level of zinc oxide in weaning pigs but also ideal dietary SNPro level was 0.02%.

This study was conducted to determine the effects of dietary protein level and supplementation of protease on growth performance, nutrient digestibility, gut microflora, intestinal morphology and fecal noxious gas emission in weanling pigs. A total of 240 weaned pigs (Landrace×Yorkshire×Duroc, 5.82±0.3 kg) were used during 4 weeks in 2 phases (days 0-14, phase 1; and days 15-28, phase 2) feeding program based on age and initial body weight. Pigs were allocated to 2×2 factorial arrangement, including 2 protein levels (HP, high protein; LP, low protein) and 2 protease levels (with or without protease). The average daily gain in the LP treatment (357 g/d) was increased rather than the HP treatment (339 g/d). A greater avarage daily gain was observed in dietary suppiemented protease treatment (358 vs 339 g/d). Average feed intake was greater in the LP treatment (544 g/d) rather than the HP treatment (530 g/d). A greater average daily feed intake was observed in dietary supplemented protease treatment (552 vs 523 g/d). Dry matter and crude protein digestibility were increased in dietary supplemented protease treatment (82.62% and 76.08%, respectively) rather than non-supplemented treatment (81.74% and 75.13%, respectively). Ileal Lactobacillus spp. count increased in dietary supplemented protease treatment (7.42 vs 7.32 log10CFU/g). Emission of H2S was decreased in the LP treatment (4.41 ppm) rather than HP treatment (4.78 ppm). Emission of NH3 was decreased in dietary supplemented protease treatment (10.43 ppm vs 11.76 ppm). In conclusion, the decrease of dietary protein level and supplementation of protease had beneficial effects on growth performance, nutrient digestibility, gut microflora, and noxious gas emission in weanling pigs.

본 연구에서는 인체 내 장내 세균 및 병원성 식중독 세균에 선인장 추출물이 미치는 영향과 항산화, 항고혈압 활성에 대하여 분석하였다. 항균활성은 96well- plate법과 paper disc법을 이용하여 실험하였으며 줄기 추출물의 경우 96well-plate 및 paper disc법의 결과, 장내유해균인 Eubacterium limosum 및 Clostridium perfringens, C. butyricum, C. difficile과 Staphylococcus aureus에 대하여 높은 억제율을 보였으나 Bacteroides fragilis, Bifidobacterium bifidum, Lactobacillus acidophilis, Streptococcus thermophilus의 경우 억제율을 나타내지 않았다. 열매 추출물의 경우 장내유해균 및 장내유익균에 Bacteroides fragilis, Bifidobacterium bifidum, Lactobacillus acidophilis, and Streptococcus thermophilis에 대하여 억제율을 나타내지 않았다. 또한 줄기 추출물의 경우 열 및 pH에서도 장내미생물에 대한 항균활성에 안정함을 나타내었다. 선인장 추출물의 폴리페놀과 플라보노이드 함량 및 DPPH 전자공여능을 측정한 결과 열처리하지 않은 열매와 줄기 추출물의 폴리페놀은 3.02 mg/g, 5.85 mg/g, 플라보노이드는 1.04 mg/g, 2.1 mg/g, 전자공여능은 87, 88%로 높은 수율을 나타내었다. 선인장 추출물의 항고혈압활성은 줄기 추출물의 경우 88.8% 열매 추출물의 경우 69.2%의 항고혈압활성을 보였으며 상기 결과를 종합할 때, 선인장 줄기추출물은 장내 유해균의 억제 및 유익균의 증진을 통한 장내 균총 개선을 위한 기능성 식품소재로 활용할 수 있다고 판단된다.

We evaluated the anti-allergic effect of black colored rice (Oryza sativa L.) and its main constituents, cyanidin 3 glucoside (C3G) and cyanidin (Cy) on scratching behavior in mice. The black rice extract (BRE) and its constituts inhibited the scratching behavior induced by compound 48/80 and histamin. To understand the role of intestinal microflora in the anti-allergic effect of C3G, which was suspected to play a key role in depressing scratching behavior, C3G was incubated with human fecal bacteria. Protocatechuic acid (PCA) was found within the 30 min and its concetration sharply increased. Vanillic acid (VA) was also detected in the incubated sample mixture. The anti-allergic effect of these compounds, C3G, Cy, PCA and VA, were investigated. PCA and Cy potentely reduced scratching behavior in mice induced by compound 48/80 and histamin. These findings enable us to conclude that intestinal bacterial metabolites, PCA and VA, produced by intestinal micro flora contributed to suppressing the scratching behavior induced by compound 48/80 and histamin.

장내미생물 개선에 효과가 있는 약용식물소재들을 탐색하여 정장제품 개발하고자 13종의 식물을 물과 에탄올로 각각 추출하여 농도별로 유해균인 C. perfringens, E. coli와 유익균인 B. longum의 생육에 미치는 영향을 in vitro실험으로 시험한 결과는 아래와 같다. 가. C. perfringens은 민들레와 인진쑥 물 추출물에, 돌나물, 오미자, 민들레, 부추, 생강, 쑥, 인진쑥, 질경이 에탄올추출물에 강하게 생육억제 되었다. 나. E. coli은 돌나물, 오미자 물과 에탄올 추출물에서 강하게 생육억제 되었다. 다. B. longum은 추출용매에 관계없이 생육에는 크게 영향을 주지 않았다. 라. 돌나물과 오미자 에탄올추출물이 유익균에 생육억제 없이, 두 유해균을 억제됨으로서 이들 소재가 정장제품으로의 개발 가능성이 있다고 생각되었다.