The purpose of this study was to examine the appearance of norovirus in the water for food in food service institutions and the influence of physicochemical and microbial factors of norovirus in order to work out basic data to predict the detection of norovirus. Among 82 samples of water for food in food service institutions, norovirus appeared in 7 samples and the rate of appearance was 8.5%. As for the type of norovirus, one samples contained GI type (genotype GI-6) and six samples contained GII type (genotype GII-2, GII-4, GII-12). In the regression model of prediction of norovirus, the rate of appearance was correlated with NH₃-N, total solids and the consumption of KMnO₄, out of such variables as NH₃-N, total solids, the consumption of KMnO4, depth, chloride and total colony counts, and its contribution rate for effectiveness was 78.60%. In order to examine the influential factor of environment upon the detection of norovirus, Pearson's correlation analysis was carried out. The predictable regression formula for appearance rate of norovirus was expressed as -1.818 + 42.677 [NH₃-N] + 0.023 [total solids] + 0.762[consumption of KMnO₄] -0.009 [depth] -0.146 [chloride] + 0.007 [total colony counts] (R = 0.904, R² = 0.818,adjusted R² = 0.786, p < 0.05). The most influential factors upon the detection of norovirus were NH₃-N, total solids and the consumption of KMnO₄. In other words, when the measured values of NH₃-N, total solids and the consumption of KMnO₄ were higher, the possibility of appearance of norovirus increased.
The occurrence trends and moleculargenetic characteristics of noroviruses detected from gastroenteritis patients in Jeju from 2008 to 2010 were investigated. In addition, the norovirus contamination and its characteristics of groundwaters in Jeju were examined. The incidence caused by norovirus in viral gastroenteritis patients has increased every year and was higher in male than in female. The patients caused by norovirus occurred throughout all months. The incidences started to increase from November, were very high from December to February, started to decrease from March, and were very low from June to September. The patients caused by norovirus occurred throughout all ages, however, the infants below 5 years were the most susceptible to norovirus infection and the age group from teens to forties were the most insensitive to norovirus infection. The sequencing analysis showed that 18 genotypes (8 genogroup I (GI) and 10 genogroup II (GII)) were detected, the incidences caused by GI and GII were 11.5% and 88.5%, respectively, and predominant genotype was GII-4 (70.5%), which was the major genotype giving rise to norovirus incidences in Jeju, together with GII-3 (6.1%) and GI-4 (4.1%). Among 20 groundwaters sampled at 9 wells (4 non-drinking water wells and 5 drinking water wells), noroviruses were detected from 2 groundwaters sampled at one non-drinking water well and their genotypes were GI-5 and GI-8.