토끼에서는 수정란 이식과 같은 기본적인 번식공학적 방법의 효율성이 아직 생쥐와 같은 실험동물에 비해 떨어지고 있어 생물공학적인 기술을 응용하는데 큰 어려움이 있다. 특히 유전자 이식에 의한 형질전환 토끼의 생산과 같은 생물공학적인 기술을 실용화하는데 효율이 높은 수정란이식 기술의 개발이 필수적이라고 할 수 있다. 본 연구에서는 토끼에서 수정란 이식 기술의 첫 단계인 과배란 유도를 효율적으로 이용할 수 있는 방법을 정립하기 위해 반복적인 과배란 유도가 배

The present work was designed to understand the mechanism of superovuiation and the cause of early embryo loss and Implantation failure in the superovulating immature female rats which were elaborated by a pituitary gland transplantation. A pituitary gland obtained from the orchidectomized rats was transplanted under the right kidney capsule of 28 day old female rats (PGT group) on the starting day of experiment which was designated as Day 2. The grafted pituitary glands were removed at 6h (RPGT 6h group), 12h (RPGT 12hgroup) and 24h (RPGT 24h group) after the transplantation. Control rats were treated with 41U PMSG on Day 2 (PMSG group). The estrous cycle and the levels of plasma progesterone and estradiol-17 were observed on Day 0, Day 5, respectively. The implantation sites, the weights of ovary and uterus, and the number of corpora lutea were examined in all group on Day 8. The resuft obtained were summarized as follows: 1. The percentages of the number of the rats in proestrus and estrus were 93.0%, 82.6%, 0%, 90.7% and 89.5% in PMSG, PGT, RPGT6h, RPGT12h and RPGT24h group, respectively. The synchronization of estrus cycle was dchieved in all groups. 2. The mating rates of each group were 80.2, 75.0, 0, 56.4, 57.8% in PMSG, PGT, RPGT6h, RPGT12h, RPGT24h group, respectively. 3. The numbers of copora lutea on Day 8 were 47.1 i 4.9, 18.1 0.5, 14.1 i 0.3 and 8.9 0.3 in PGT, RPGT24h, RPGTl2h and PMSG group, respectively. There were signIficantly difference between all groups (P<0.05). 4. The numbers of implantation sites (18.1 +- 4.0) in PGT group on Day 8 were higher than those of PMSG (8.5 2.5), RPGT 12h (9.8 i 0.2) and RPGT 24h group (10.8 i 0.2) (P<0.05). 5. The ovarlan weights in PGT (95.2 14.3mgIlOOg BW), RPGT 12h (51.7 0.6mgIlOOg BW), and RPGT24h (57.9 0.9mg/l00g 8W) groups were significantly higher than those of PMSG group (30.4 7.4mg/l00g BW) (P<0.05). 6. The uterine weights in PMSG (672.4 4.7mg/l00g 8W), and PGT (660.7 7.8mg/l00g BW) groupswere greater than those of RPGT 12h (403.0 1.lmg/lOOg BW) and RPGT 24h (490.1 0.9mg/l00g BW) group (P<0.05). 7. The plasma progesterone levels in PGT groups (15 lng/ml) on Day 5 were higher than those of PMSG (83ng/ml), RPGT 12h (S7ng/ml), RPGT24h (8lng/ml) group (P<0.05). 8. The plasma estradiol-17 levels in PMSG group (lBSpg/ml) on Day S were higher than those of RPGT 24h (l3pg/ml) group (P<0.05). But estradiol-17 levels in PGT and RPGT 12h group were too low to discuss.

The effects of ovulation induction in ussurian bullhead, Leiocassis ussuriensis, were investigated by treating ussurian bullhead with hCG, LHRHa, GnRHa, ovaprim, and pimozide. hCG was injected to ussurian bullhead at 0.75% NaCl, 5,000, 10,000, 20,000, and 30,000 IU, respectively. The ovulation inducement rates were 100% in 20,000 and 30,000 IU. Fertilization rates were 82.7% and 79.8%. Hatching rates were 59.4% and 57.2%. Ovulation time was between 16-19 hr The concentrations of LHRHa injected were 0.75 NaCl, 50, 100, 200, 300, and . The ovulation inducement rates were 100% in 300 and . Fertilization and hatching rates were 84.9% and 68.4% at . The times to ovulation were between 23 hr and 34 hr. Ovaprim of 0.75% NaCl, 1.0, 1.5, 2.0, 2.5 and 3.0 ml/kg were injected to the abdominal cavity. The ovulation inducement rate was highest at 2.0 and 3.0 ml/kg to 92% and ovulation time was between 27-38 hr. LHRHa concentrations of 0.75% NaCl, 50, 100, 200, 300 and were injected with pimozide (). Ovulation inducement rate was 100% from 200 to 400 IU with pimozide. Ovulation time was 22-36 h. Fertilization and hatching rates were 88.9% and 70.4% in with pimozide.

본 연구에서는 각각의 호르몬 처리에 따른 배란유도 효과를 알아보고자 하였다. LHRHa 호르몬을 종어 암컷에 2회 투여 시 50과 100 농도에서 100%의 배란율을 보여 가장 높았으며, LHRHa를 1차 처리한 후, 인간 태반성 성선자극호르몬인 HCG를 2차 처리하였을 때, 500 IU에서는 배란이 이루어지지 않았으며, 2,000 IU를 주사한 암컷에서 72%의 높은 배란율을 보였다. HCG만 처리했을 시 500~25,000 IU까지 각각 10, 17

양식산 농어, Lateolabrax japonius 암컷의 난소와 혈액을 1997년부터 1999년까지 매년 10월부터 2월까지 2회 반복 채취하였다. Gonadosomatic index는 11월부터 증가하기 시작하여 12월(12.81.5)과 1월(14.83.5)에 최고 수준을 나타낸 후 2월(2.61.5)에는 급격히 감소하였다(P<0.05). 난소내 난모세포는 12월과 1월에 3차 난황구기까지 발달하고 성장이 완료되지만, 성숙 및 배란이 되지 않고 2월