Developments in cancer therapies and diagnostic techniques have improved the long-term survival of cancer patients. Certain cancer treatments, such as radiotherapy, often harm normal tissue as well as the specifically targeted cancer cells. High doses of radiation induce bone loss. This study investigated the effects of pentoxifylline (PTX) on radiation-induced bone loss in C3H/HeN mice. C3H/HeN mice were divided into sham and irradiation (3 Gy, gamma-ray, IR) groups. The irradiated mice were treated for 12 weeks with vehicle, PTX (p.o.) or PTX (s.c.). Grip strength, uterus weight, serum alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) level were measured. Tibiae were analyzed using micro-computed tomography. There were no significant differences in the degree of grip strength, body weight and uterine weight between IR group and PTX-treated group. Treatment of PTX significantly preserved trabecular bone volume, trabecular number, trabecular separation and bone mineral density of proximal tibia metaphysic. The administration of PTX lowered serum TRAP in IR mice, suggesting that PTX can reduce the bone resorptive rate in mice. Our experimental data support the protective role of PTX against bone loss in irradiated mice. Based on the findings of this study, development of PTXbased treatments is anticipated to address bone loss after radiotherapy. Prospective dose escalation studies are required to determine the appropriate dosage of PTX.

This study was conducted to determine the effect of pentoxifylline levels on sperm motility, survival rate, sperm membrane integrity of frozen semen and fresh-extended equine semen in Jeju cross-bred horses. As a result of sperm characteristic comparison depending on pentoxifylline levles at 30 minutes post-thaw, the progressive motilities were 53.25±2.87 (4mM pentoxifylline) and 50.28±2.14 (8mM pentoxifylline) and significantly higher compared to the control group(40.09±5.15) and other treatment group (16mM pentoxifylline, 41.27± 2.82). The progressive fast motility were 22.44±1.62 (4mM pentoxifylline,) and 22.74±3.07 (8mM pentoxifylline) and significantly higher compared to the control group (13.47±1.48) and other treatment group (16mM pentoxifylline, 14.66±3.68) (p<0.05). As a result of sperm characteristic comparison depending on pentoxifylline levles at 30 minutes post-thaw were 68.96±1.64 (4mM pentoxifylline) and 67.90±6.72 (8mM pentoxifylline) and significantly higher compared to the control group (53.48±4.84) and other treatment group (16mM pentoxifylline, 58.14±2.65) (p<0.05). In conclusion, these results suggest that treatment groups with 4mM and 8mM pentoxifylline were higher compare to equine seperm mobility and the control group and treatment groups with more than 16mM pentoxifylline has a negative effect on sperm characteristics. After thawing, the total motility in post-thawed equine sperm has increased by 10 percent for 1 hour. these results suggest that pentoxifylline contributes to the improvement of the equine sperm motility and characteristics in post-thawed semen.

국내 승용마 산업 발전과 더불어 인공수정 기술을 활용하여 생산된 승용마 두수가 증 가하고 있다. 말 인공수정용 정액은 신선정액, 냉장정액, 냉동정액의 형태로 활용된다. 말 동결정액은 타축종과는 달리 개체 차이가 크고 해동 후 운동성이 크게 낮아지는 문제 점 때문에 인공수정 시 신선정액 또는 냉장정액을 이용하는 비율이 높다. 본 연구에서는 정장 제거 후 정액 냉장보관 시 pentoxifylline이 미치는 효과를 구명하기 위하여 수행하 였다. 본 실험은 제주산마 3두를 공시하였으며, 정액채취는 예비 채취 후 일주일 간격으 로 각 2회 수행하였다. pentoxifylline 1mM, 2mM, 3mM, 4mM이 포함된 INRA96 희석제 에 희석 후 4℃에 보관하며 평가를 위해 정자의 운동성, 생존율, 원형질막 온전성을 24시 간 간격으로 조사하였다. 운동성과 직진 운동성은 CASA 분석 시스템을 이용하여 분석하 였고 생존율을 검사를 위하여 eosin-nigrosin 염색과 원형질막 온전성 검사를 위하여 HOSTest가 수행되었다. 본 실험에서는 대조군에 비해 pentoxifylline 1mM, 2mM 첨가군 이 48시간 이후 운동성과 직진 운동성에서 더 높은 경향을 보였으며, 생존율과 원형질막 온전성에서 24시간 측정 결과 대조군에 비해 pentoxifylline 2mM첨가군이 높은 경향을 보였다. 본 연구결과 향후 정자의 수정능력에 대한 연구가 추가적으로 이루어져야 할 것 으로 고려된다.