In order to investigate phenotype and genotype of Salmonella enterica subsp. enterica serovar Enteritidis, Forty-eight S. Enteritidis isolates from diarrhea patients were analysed using antimicrobial resistance typing, Phage typing, and Pulsed field gel electrophoresis in Seoul from 2004 to 2005. All of S. Enteritidis were resistant to streptomycin(SM, 37.5%), ampicillin(AM, 43.8%), t icarcillin(TIC, 43.8%), chloramphenicol(CM, 29.2%), t etracycline(TE, 10 .4%) and nalidixic acid(NA, 18.8%) among 16 antimicrobial drugs. Of 48 S. Enteritidis, 8 isolates(16.7%) were resistant to 1 drug, 3 isolates( 6.3%) to 2 drugs, 1 isolate (2.1%) to 3 drugs and 17 isolates(35.7%) to 4 drugs. The basic pattern of 4 drugs resistance was SM, TIC, TE, and CM but 1 drug resistant isolates represent all nalidixic acid resistance. Among 30 antibiotic r esistant S . Enteritidis, 2 1 isolates(70 %) were phage type 2 1, 8 i solates(26.7%) were phage t ype 2 3 and 1 isolate( 3.3%) was RDNC, respectively. Of the phage types observed, all of phage type 23(8 isolates) were nalidixic acid resistant and phage type 21 were AM-TIC-SM-CM multi-resistance(13 isolates; 43.3%), AM-TIC-SM-TE(4 isolates; 13.3%), AM-TIC-SM(1 isolate; 3.3%), AM-TIC-CM(1 isolate; 3.3%), and AM-TIC(2 isolates; 6.7%) resistance and 1 isolate of RDNC was NA-TE resistance. PFGE divided the isolates into two major clusters, A(n=14) and B(n=14). There were four different resistance profiles with resistance to AM, TIC, SM, TE, NA within PFGE A. Also resistance to AM, TIC, SM, CM was common within PFGE B. The PFGE A strains typed as PT21(n=5), PT23(n=8), and RDNC(n=1), While all the PFGE B strains typed as PT21(n=14). In consequence, there was the highly significant concurrence between resistance typing, phage typing and PFGE.

To investigate antimicrobial susceptibility and relationships of the multidrug resistance and phage types of 49 Salmonella enterica serovar Typhimurium isolated from diarrhea patients in Seoul between 1999 and 2002, we analyed stranis by serotyping, antimicrobial susceptibility test and phage typing. Out of 2,705 samples examined for the causative agent from diarrhea patients, total of 512 isolates were confirmed to be genus Salmonella. Out of 512 isolates, 49 isolates(9.6%) were identified as S. Typhimurium. All of the S. Typhimurium strains represented 100% susceptibility to ceftriaxone, cefoxitin, amikacin and ciprofloxacin and were over 90% susceptibility to gentamicin, cephalothin and kanamycin, but were resistant to tetracycline(75.5%), streptomycin(59.2%), sulfonamides(55.1%), ticarcillin(36.7%), ampicillin( 28.6%), and chloramphenicol(20.4%). Out of 49 S. Typhimurium, only 3 isolates(20.4%) were resistant to one drug, 6 isolates(12.2%) to two drugs and 12 isolates(24.5%) to 3 drugs, 1 isolate(2.0%) to 4 drugs, 2 isolates (4.1%) to 5 drugs, and 6 isolates(12.2%) to 6 drugs and 5 isolates (10.2%) to 7 drugs. Out of 49 S. Typhimurium, 10 isolates(20.4%) were DT195, 5 isolates(10.2%) were DT193 and DT206, 4 isolates(8.2%) were DT104L, DT146, DT203 and RDNC, respectively. Phage types observed the resistant patterns of more than 6 drugs were DT104L, DT193, DT194, DT195 and DT67, but those of 3 drugs representing S-S3-TE type was DT195.